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Rabbit Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

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Images

Sandwich ELISA - Anti-Neurogranin (Trunc P75) antibody [EPR22522-19] - BSA and Azide free (Capture) (AB277261), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Expected

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22522-19

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab277261 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurogranin. The recommended pair for sandwich ELISA is: Capture: ab277261, Human Neurogranin (Trunc P75) Capture Antibody (unconjugated) Detector: Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) ab277260, Human Neurogranin (Trunc P75) Detector Antibody (unconjugated)The reference range value is 25 - 400 pg/ml.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neurogranin (Trunc P75) sometimes referred to as NRGN is a small molecular weight protein with a mass around 7.5 kDa. This protein is abundant in the brain specifically in the dendritic spines of neurons in regions like the hippocampus and cerebral cortex. Neurogranin plays a mechanical role by binding to calmodulin in a calcium-dependent manner when neurons are at rest. This action helps regulate calmodulin availability which affects various downstream signaling cascades.

Biological function summary

Neurogranin influences synaptic plasticity and cognitive functions like learning and memory. It acts as a calmodulin-binding protein that affects the formation of protein complexes important for the aforementioned neural processes. Studies show that its localization in postsynaptic compartments suggests a role in modulating synaptic strength. Neurogranin facilitates signal transduction processes that underlie synapse function and plasticity and interact with phosphatidylinositol signaling pathways.

Pathways

Neurogranin functions within the calcium signaling pathway and the protein kinase C (PKC) pathway. It modulates calcium/calmodulin-dependent protein kinase II (CaMKII) through calmodulin regulation influencing synaptic plasticity and memory formation. Additionally its interaction with PKC contributes to the regulation of several cellular responses including cell proliferation and differentiation. This connection to signaling pathways highlights its importance in maintaining neuronal communication and health.

Associated diseases and disorders

Neurogranin's altered expression and function relate to cognitive disorders such as Alzheimer's disease and schizophrenia. Neurogranin levels often decrease in Alzheimer's disease potentially disrupting synaptic function and contributing to cognitive decline. This protein also interacts with pathways involving amyloid precursor protein (APP) and tau proteins which are associated with neurodegenerative conditions. In schizophrenia altered Neurogranin expression could affect synaptic plasticity and neurotransmitter regulation linking it to pathophysiological mechanisms of the disorder.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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