Rabbit Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
sELISA | |
---|---|
Human | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Acts as a 'third messenger' substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium (By similarity).
Neurogranin, Ng, RC3, NRGN
Rabbit Recombinant Monoclonal Neurogranin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR22522-19
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab277261 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Neurogranin. The recommended pair for sandwich ELISA is: Capture: ab277261, Human Neurogranin (Trunc P75) Capture Antibody (unconjugated) Detector: Anti-Neurogranin antibody [EPR22523-107] - BSA and Azide free (Detector) ab277260, Human Neurogranin (Trunc P75) Detector Antibody (unconjugated)The reference range value is 25 - 400 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This supplementary information is collated from multiple sources and compiled automatically.
Neurogranin (Trunc P75) sometimes referred to as NRGN is a small molecular weight protein with a mass around 7.5 kDa. This protein is abundant in the brain specifically in the dendritic spines of neurons in regions like the hippocampus and cerebral cortex. Neurogranin plays a mechanical role by binding to calmodulin in a calcium-dependent manner when neurons are at rest. This action helps regulate calmodulin availability which affects various downstream signaling cascades.
Neurogranin influences synaptic plasticity and cognitive functions like learning and memory. It acts as a calmodulin-binding protein that affects the formation of protein complexes important for the aforementioned neural processes. Studies show that its localization in postsynaptic compartments suggests a role in modulating synaptic strength. Neurogranin facilitates signal transduction processes that underlie synapse function and plasticity and interact with phosphatidylinositol signaling pathways.
Neurogranin functions within the calcium signaling pathway and the protein kinase C (PKC) pathway. It modulates calcium/calmodulin-dependent protein kinase II (CaMKII) through calmodulin regulation influencing synaptic plasticity and memory formation. Additionally its interaction with PKC contributes to the regulation of several cellular responses including cell proliferation and differentiation. This connection to signaling pathways highlights its importance in maintaining neuronal communication and health.
Neurogranin's altered expression and function relate to cognitive disorders such as Alzheimer's disease and schizophrenia. Neurogranin levels often decrease in Alzheimer's disease potentially disrupting synaptic function and contributing to cognitive decline. This protein also interacts with pathways involving amyloid precursor protein (APP) and tau proteins which are associated with neurodegenerative conditions. In schizophrenia altered Neurogranin expression could affect synaptic plasticity and neurotransmitter regulation linking it to pathophysiological mechanisms of the disorder.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Representative standard curve from corresponding SimpleStep ELISA® Kit (Human Neurogranin ELISA Kit (Trunc P75) (NRGN) ab277084).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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