AB186279

Anti-Neuroligin 1 antibody [N97A/31]

Name: Anti-Neuroligin 1 antibody [N97A/31] (ab186279) | Abcam
Brand: Abcam Limited
SKU: AB186279
Price: 510 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal Neuroligin 1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nlgn1 aa 700 to C-terminus.

View Alternative Names

KIAA1070, NLGN1, Neuroligin-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 1 antibody [N97A/31] (AB186279)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 1 antibody [N97A/31] (AB186279)

SK-N-BE (human neuroblastoma cell line) cells stained for Neuroligin 1 (green) using ab186279 at 1/100 dilution in ICC/IF. The secondary antibody is Goat Anti-Mouse ATTO 488. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red (red).

Supplier Data

Western blot - Anti-Neuroligin 1 antibody [N97A/31] (AB186279)

All lanes:

Western blot - Anti-Neuroligin 1 antibody [N97A/31] (ab186279) at 1/200 dilution

All lanes:

Mouse brain membrane lysate

Secondary

All lanes:

Goat Anti-Mouse IgG-HRP at 1/1000 dilution

Predicted band size: 94 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N97A/31

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rat Nlgn1 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q62765

Specificity

ab186279 does not cross-react with other Neuroligins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from S97A-31 to N97A/31, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuroligin 1 also known as NLGN1 is a member of the neuroligin family of cell adhesion molecules. It plays a significant mechanical role at synaptic junctions where it mediates synapse formation and maintenance. Neuroligin 1 weighs approximately 97 kDa and is mainly expressed in the brain particularly in postsynaptic densities of excitatory glutamatergic synapses. Neuroligin 1 interacts with neurexins on the presynaptic side facilitating trans-synaptic connections important for proper neuronal communication.

Biological function summary

Neuroligin 1 contributes to synaptic organization and signaling by being part of a larger protein complex. This complex involves postsynaptic scaffolding proteins such as PSD-95 which anchor neuroligin to the cytoskeleton and receptors like NMDA and AMPA receptors. Neuroligin 1 regulates synaptic stabilization and plasticity which are essential for learning and memory processes. Through these interactions it impacts synaptic strength and efficiency playing an integral role in the modulation of synaptic activity.

Pathways

Neuroligin 1 is a critical component of synaptic development and function pathways. It operates within the synaptic signaling and plasticity pathways affecting neurotransmitter function and reception. Neuroligin 1 functions in coordination with proteins such as neurexins and SHANK proteins to regulate the assembly and maturation of synapses. These interactions influence calcium signaling and involve the recruitment of other synaptic proteins that further contribute to synaptic modulation.

Neuroligin 1 is linked to neurological conditions like autism spectrum disorders (ASD) and schizophrenia. Mutations or dysregulations of neuroligin 1 impact synaptic connectivity leading to altered neuronal networks and communication deficits involved in these conditions. The balance of excitatory and inhibitory synaptic function that neuroligin and its binding partners including neurexins and SHANK3 control is directly implicated in the pathophysiology of these disorders. Disruptions in neuroligin-neurexin signaling pathways may therefore contribute to the synaptic dysfunctions observed in ASD and schizophrenia.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (By similarity). The protein is involved in nervous system development.

See full target information NLGN1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Drug design, development and therapy 17:1175-1189 PubMed37113470

2023

Up-Regulation of miR-9-5p Inhibits Hypoxia-Ischemia Brain Damage Through the DDIT4-Mediated Autophagy Pathways in Neonatal Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Gai,Xiaohui Xing,Yan Song,Yijing Zhao,Zige Jiang,Yahong Cheng,Yilei Xiao,Zhen Wang

Cellular and molecular life sciences : CMLS 79:294 PubMed35562616

2022

Prenatal glucocorticoid exposure selectively impairs neuroligin 1-dependent neurogenesis by suppressing astrocytic FGF2-neuronal FGFR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gee Euhn Choi,Chang Woo Chae,Mo Ran Park,Jee Hyeon Yoon,Young Hyun Jung,Hyun Jik Lee,Ho Jae Han

Neural plasticity 2020:8815195 PubMed32802036

2020

Neuroprotective Effects of the Sonic Hedgehog Signaling Pathway in Ischemic Injury through Promotion of Synaptic and Neuronal Health.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Yin,Xuemei Bai,Danqing Xin,Tingting Li,Xili Chu,Hongfei Ke,Min Han,Wenqiang Chen,Xingang Li,Zhen Wang

Cellular and molecular neurobiology 40:617-628 PubMed31760535

2019

Increased Fibronectin Impairs the Function of Excitatory/Inhibitory Synapses in Hirschsprung Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Gao,Peimin Hou,Jian Wang,Tingting Zhou,Dongming Wang,Qiangye Zhang,Weijing Mu,Xiaona Lv,Aiwu Li

Experimental and therapeutic medicine 17:3117-3123 PubMed30936983

2019

NL1 expression level in Nrx1β and the excitability of PV interneurons in mice with POCD.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Tian,Yuan Zhou,Lili Qiu,Fangfang Liu,Li Jin,Weiyan Li,Lidong Zhang

Scientific reports 8:3533 PubMed29476096

2018

Low-level Gestational Lead Exposure Alters Dendritic Spine Plasticity in the Hippocampus and Reduces Learning and Memory in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zai-Hua Zhao,Gang Zheng,Tao Wang,Ke-Jun Du,Xiao Han,Wen-Jing Luo,Xue-Feng Shen,Jing-Yuan Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neuroligin 1 antibody [N97A/31]

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 1 antibody [N97A/31] (AB186279)

Western blot - Anti-Neuroligin 1 antibody [N97A/31] (AB186279)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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