AB181251

Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal NLGNY antibody. Suitable for WB and reacts with Human, Recombinant fragment - Human samples. Cited in 4 publications.

View Alternative Names

KIAA0951, NLGN4Y, Neuroligin Y, KIAA1070, NLGN1, Neuroligin-1

2 Images

Supplier Data

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (AB181251)

All lanes:

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (ab181251) at 1/2000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Hela lysate at 20 µg

Lane 3:

A549 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 92 kDa

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Lab

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (AB181251)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (ab181251) at 1/1000 dilution

Lane 1:

His-tagged human recombinant Neuroligin 1 protein at 10 ng

Lane 2:

His-tagged human recombinant Neuroligin 2 protein at 10 ng

Lane 3:

His-tagged human recombinant Neuroligin 3 protein at 10 ng

Lane 4:

His-tagged human recombinant Neuroligin 4 protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 120 kDa

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Exposure time: 5s

Carrier free

Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13108

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen of this antibody shows 92% sequence homology with the human Neuroligin-1 (UniProt ID Q8N2Q7) and 77% with human Neuroligin-2 (UniProt ID Q8NFZ4) and Neuroligin-3 (UniProt ID Q9NZ94)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuroligin 1 and Neuroligin 4 are cell adhesion proteins that play an important role in the development and function of synaptic connections in the nervous system. These proteins are part of the neuroligin family with Neuroligin 1 sometimes referred to as NLGN1 and Neuroligin 4 as NLGN4. Neuroligin 1 has a molecular mass of approximately 110 kDa while Neuroligin 4 has a mass of about 100 kDa. These proteins are primarily expressed in neurons where they contribute to the formation and maintenance of synaptic junctions between neurons.

Biological function summary

Neuroligin 1 and Neuroligin 4 facilitate the formation and maturation of excitatory and inhibitory synapses in the brain. These proteins work as part of a complex with their binding partners neurexins which reside on the presynaptic membrane to bridge the synaptic cleft. This interaction helps stabilize synaptic contacts and promotes the balance of synaptic transmission. Their role in synaptic specialization highlights their significance in maintaining proper neuronal network communication.

Pathways

Neuroligin 1 and Neuroligin 4 are positioned within the synaptic signaling pathways notably in the involvement of synapse organization and plasticity. These pathways ensure that synaptic signals are correctly transmitted and received which is essential for learning and memory processes. Neuroligins interact closely with the SHANK proteins and PSD-95 which are important components of the postsynaptic density. This interaction contributes to the structural and functional integrity of synapses allowing for precise cellular communication.

Mutations in Neuroligin 1 and Neuroligin 4 have been linked to neural developmental disorders including autism spectrum disorders and X-linked mental retardation. Aberrant neuroligin function affects synaptic stability and connectivity contributing to the pathophysiology of these conditions. Studies have identified interactions with proteins like neurexins and SHANK3 to be significant highlighting the complexity of neuronal circuitry affected in these disorders. Understanding this connection is key for exploring therapeutic strategies targeting synaptic dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members.

See full target information NLGN4Y

Additional targets

NLGN1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

eNeuro 12: PubMed40032531

2025

Phosphorylation of NLGN4X Regulates Spinogenesis and Synaptic Function.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander W Lehr,Thien A Nguyen,Wenyan Han,Eunhye Hong,John D Badger,Wei Lu,Katherine W Roche

Cellular oncology (Dordrecht, Netherlands) 47:897-915 PubMed38082211

2023

CSGALNACT2 restricts ovarian cancer migration and invasion by modulating MAPK/ERK pathway through DUSP1.

Applications

Unspecified application

Species

Unspecified reactive species

Mingjun Ma,Chao Wang,Meixuan Wu,Sijia Gu,Jiani Yang,Yue Zhang,Shanshan Cheng,Shilin Xu,Minghai Zhang,Yongsong Wu,Yaqian Zhao,Xiu Tian,Dominic Chih-Cheng Voon,Chiaki Takahashi,Jindan Sheng,Yu Wang

Human molecular genetics 31:674-691 PubMed34542148

2021

Neuroligin-3 and neuroligin-4X form nanoscopic clusters and regulate growth cone organization and size.

Applications

Species

Unspecified reactive species

Nicholas J F Gatford,P J Michael Deans,Rodrigo R R Duarte,George Chennell,Katherine J Sellers,Pooja Raval,Deepak P Srivastava

Neuron 106:759-768.e7 PubMed32243781

2020

A Cluster of Autism-Associated Variants on X-Linked NLGN4X Functionally Resemble NLGN4Y.

Applications

Unspecified application

Species

Unspecified reactive species

Thien A Nguyen,Kunwei Wu,Saurabh Pandey,Alexander W Lehr,Yan Li,Michael A Bemben,John D Badger,Julie L Lauzon,Tongguang Wang,Kareem A Zaghloul,Audrey Thurm,Mahim Jain,Wei Lu,Katherine W Roche

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108]

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (AB181251)

Western blot - Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (AB181251)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (4)

Product promise