Anti-Neuroligin 2 antibody [EPR28230-17]

9 product images

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  Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This antibody does not cross-react with mouse Neuroligin 3 and Neuroligin 1.

  In Western blot, Anti-His tag® antibody staining at 1/5000 dilution.

  All lanes: Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094) at 1/1000 dilution

  Lane 1: Purified mouse Neuroligin 2 recombinant protein at 20 ng

  Lane 2: Purified His-tagged mouse Neuroligin 1 recombinant protein at 20 ng

  Lane 3: Purified His-tagged mouse Neuroligin 3 recombinant protein at 20 ng

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 91 kDa

  Exposure time: 7.75s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on mouse cerebrum (PMID: 19926856).The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on rat cerebrum (PMID: 16982420).The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Immunohistochemical analysis of paraffin-embedded: (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse NLGN2 expression vector containing a his tag. (B) HEK-293T cells transfected with a mouse NLGN1 expression vector containing a his tag. (C) HEK-293T cells transfected with a mouse NLGN3 expression vector containing a his tag. (D) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Positive staining on (A) HEK-293T cells transfected with a mouse NLGN2 expression vector containing a his tag, no staining on (B) HEK-293T cells transfected with a mouse NLGN1 expression vector containing a his tag, and (C) HEK-293T cells transfected with a mouse NLGN3 expression vector containing a his tag, and (D) HEK-293T cells transfected with empty vector containing a his tag.The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on mouse liver.The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Negative control: no staining on rat liver.The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunoprecipitation - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Neuroligin 2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab315094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315094 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

  Lane 1: Mouse brain tissue lysate

  Lane 2: ab315094 IP in Mouse brain tissue lysate

  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315094 in mouse brain tissue lysate

  Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  All lanes: Immunoprecipitation - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094) at 1/30 dilution

  All lanes: Mouse brain tissue lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Exposure time: 180s

• 

  Dot Blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Dot blot analysis of Neuroligin 2 using ab315094 at 1:1000 (0.519 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

  Exposure time: 180 Seconds.

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  This antibody does not cross-react with mouse Neuroligin 3 and Neuroligin 1.

• 

  Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: liver, kidney (PMID: 10718198, 8576240, 18434543).

  Samples are non-boiled as boiling may cause protein aggregation.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse hippocampus tissue lysate at 20 µg

  Lane 3: Mouse cerebellum tissue lysate at 20 µg

  Lane 4: Mouse liver tissue lysate at 20 µg

  Lane 5: Mouse kidney tissue lysate at 20 µg

  Lane 6: Rat brain tissue lysate at 20 µg

  Lane 7: Rat hippocampus tissue lysate at 20 µg

  Lane 8: Rat kidney tissue lysate at 20 µg

  Lane 9: Rat liver tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 91 kDa, 36 kDa

  Exposure time: 81s