Anti-Neuroligin 2 antibody [EPR28230-17]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Immunohistochemical analysis of paraffin-embedded : (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse NLGN2 expression vector containing a his tag. (B) HEK-293T cells transfected with a mouse NLGN1 expression vector containing a his tag. (C) HEK-293T cells transfected with a mouse NLGN3 expression vector containing a his tag. (D) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T cells transfected with a mouse NLGN2 expression vector containing a his tag, no staining on (B) HEK-293T cells transfected with a mouse NLGN1 expression vector containing a his tag, and (C) HEK-293T cells transfected with a mouse NLGN3 expression vector containing a his tag, and (D) HEK-293T cells transfected with empty vector containing a his tag.The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat liver.The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum (PMID : 16982420).The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum (PMID : 19926856).The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuroligin 2 with ab315094 at 1/2000 (0.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver.The section was incubated with ab315094 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

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Immunoprecipitation - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Neuroligin 2 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab315094 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315094 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate

Lane 2 : ab315094 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315094 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094) at 1/30 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

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Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : liver, kidney (PMID : 10718198, 8576240, 18434543).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Lane 7:

Rat hippocampus tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Lane 9:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 91 kDa,36 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse Neuroligin 3 and Neuroligin 1.

In Western blot, Anti-His tag® antibody staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Neuroligin 2 antibody [EPR28230-17] (ab315094) at 1/1000 dilution

Lane 1:

Purified mouse Neuroligin 2 recombinant protein at 20 ng

Lane 2:

Purified His-tagged mouse Neuroligin 1 recombinant protein at 20 ng

Lane 3:

Purified His-tagged mouse Neuroligin 3 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 91 kDa

false

Exposure time: 7.75s

• Dot

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Dot Blot - Anti-Neuroligin 2 antibody [EPR28230-17] (AB315094)

Dot blot analysis of Neuroligin 2 using ab315094 at 1 : 1000 (0.519 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180 Seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse Neuroligin 3 and Neuroligin 1.