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AB251161

Anti-Neuroligin 3 antibody [EPR16158] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Neuroligin 3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA1480, NL3, NLGN3, Neuroligin-3, Gliotactin homolog

2 Images
Western blot - Anti-Neuroligin 3 antibody [EPR16158] - BSA and Azide free (AB251161)
  • WB

Supplier Data

Western blot - Anti-Neuroligin 3 antibody [EPR16158] - BSA and Azide free (AB251161)

This data was developed using ab192880, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Neuroligin 3 antibody [EPR16158] (<a href='/en-us/products/primary-antibodies/neuroligin-3-antibody-epr16158-ab192880'>ab192880</a>) at 1/2000 dilution

Lane 1:

Human hippocamus lysate at 20 µg

Lane 2:

Human pancreas lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa

Observed band size: 94 kDa

false

Western blot - Anti-Neuroligin 3 antibody [EPR16158] - BSA and Azide free (AB251161)
  • WB

Lab

Western blot - Anti-Neuroligin 3 antibody [EPR16158] - BSA and Azide free (AB251161)

This data was developed using ab192880, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Neuroligin 3 antibody [EPR16158] (<a href='/en-us/products/primary-antibodies/neuroligin-3-antibody-epr16158-ab192880'>ab192880</a>) at 1.916 µg/mL

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 94 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16158

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251161 is the carrier-free version of ab192880.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuroligin-3 also known as NLGN3 is a synaptic cell adhesion molecule weighing approximately 83 kilodaltons. It is expressed in the central nervous system particularly in the brain where it plays a role in synapse formation and function. The protein facilitates the connection between neurons by interacting with neurexin another synaptic protein to form a complex that links pre- and postsynaptic neurons. This interaction is essential for establishing functional synapses.
Biological function summary

A role in the central nervous system highlights the importance of NLGN3 in the development and maintenance of synaptic networks. It is part of the neuroligin family which forms complexes with neurexins across the synaptic cleft to ensure proper communication between neurons. These complexes are important for synaptic health and function playing a role in neurotransmitter receptor clustering and signal transduction which affects synaptic plasticity and strength.

Pathways

NLGN3 contributes to the regulation of synaptic plasticity and the postsynaptic density assembly pathway. It interacts with proteins such as N110 and other neuroligins which are critical for the maintenance of excitatory and inhibitory balance in neuronal circuits. This balance is significant for learning and memory processes. Neuroligin-3 through its interaction with neurexin also influences synaptic transmission by modulating the recruitment of signaling molecules at the synapse.

Abnormalities in NLGN3 are linked to neurological conditions such as autism spectrum disorders (ASD) and X-linked mental retardation. Mutations or dysregulation of NLGN3 affect synaptic function and are closely studied for their impact on neural communication in these conditions. The interaction of NLGN3 with neurexin and other neuroligins places it at the center of research focused on unraveling the pathophysiology of these disorders aiming to understand how disruption in synaptic adhesion molecules leads to cognitive and developmental challenges.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system (By similarity).
See full target information NLGN3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:4027-4050 PubMed40612676

2025

Exogenous SPD inhibits trastuzumab-mediated cardiomyocyte pyroptosis through SIRT3-regulated mitochondrial quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yu,Yan Yang,Tianzuo Chen,Qianbing Wang,Zitong Wang,Xi Gao,Qianxue Wang,Jinxiang Guo,Yuqin Wang,Yajie Zhao,Shilin Wang,Wei Lu,Xing Luo,Tielei Gao,Jiayuan Kou,Hong Li,Liming Yang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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