JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283231

Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Neuroligin 3 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

Neuroligin-3, Gliotactin homolog, Nlgn3

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)

This data was developed using ab259348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Neuroligin 3 with 259348 at 1/1000 (0.498 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining in mouse liver. The section was incubated with ab259348 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)

This data was developed using ab259348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Neuroligin 3 with 259348 at 1/1000 (0.498 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in rat cerebrum. The section was incubated with ab259348 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)

This data was developed using ab259348, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Neuroligin 3 with 259348 at 1/1000 (0.498 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in mouse cerebrum. The section was incubated with ab259348 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunoprecipitation - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)
  • IP

Supplier Data

Immunoprecipitation - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)

This data was developed using ab259348, the same antibody clone in a different buffer formulation.

Neuroligin 3 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with 259348 at 1/30 dilution (2 ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259348 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10 ug

Lane 2 : ab259348 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259348 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

All lanes:

Immunoprecipitation - Anti-Neuroligin 3 antibody [EPR23874-51] (<a href='/en-us/products/primary-antibodies/neuroligin-3-antibody-epr23874-51-ab259348'>ab259348</a>)

Predicted band size: 94 kDa

Observed band size: 110 kDa

false

Immunoprecipitation - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)
  • IP

Supplier Data

Immunoprecipitation - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)

This data was developed using ab259348, the same antibody clone in a different buffer formulation.

Neuroligin 3 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with 259348 at 1/30 dilution (2 ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259348 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10 ug

Lane 2 : ab259348 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259348 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-Neuroligin 3 antibody [EPR23874-51] (<a href='/en-us/products/primary-antibodies/neuroligin-3-antibody-epr23874-51-ab259348'>ab259348</a>)

Predicted band size: 94 kDa

Observed band size: 110 kDa

false

Western blot - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)
  • WB

Lab

Western blot - Anti-Neuroligin 3 antibody [EPR23874-51] - BSA and Azide free (AB283231)

This data was developed using ab259348, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : The molecular weight observed is consistent with what has been described in the literature (PMID : 17897391, PMID : 18755801).

Negative control : lung and liver (PMID : 17897391, PMID : 18755801).

Lanes 1-2 : 3 minutes;Lanes 3-4 : 48 seconds.

Exposure time :

All lanes:

Western blot - Anti-Neuroligin 3 antibody [EPR23874-51] (<a href='/en-us/products/primary-antibodies/neuroligin-3-antibody-epr23874-51-ab259348'>ab259348</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Rat brain tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 94 kDa

Observed band size: 110 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23874-51

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column

Product details

ab283231 is the carrier-free version of ab259348.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuroligin-3 also known as NLGN3 is a synaptic cell adhesion molecule weighing approximately 83 kilodaltons. It is expressed in the central nervous system particularly in the brain where it plays a role in synapse formation and function. The protein facilitates the connection between neurons by interacting with neurexin another synaptic protein to form a complex that links pre- and postsynaptic neurons. This interaction is essential for establishing functional synapses.
Biological function summary

A role in the central nervous system highlights the importance of NLGN3 in the development and maintenance of synaptic networks. It is part of the neuroligin family which forms complexes with neurexins across the synaptic cleft to ensure proper communication between neurons. These complexes are important for synaptic health and function playing a role in neurotransmitter receptor clustering and signal transduction which affects synaptic plasticity and strength.

Pathways

NLGN3 contributes to the regulation of synaptic plasticity and the postsynaptic density assembly pathway. It interacts with proteins such as N110 and other neuroligins which are critical for the maintenance of excitatory and inhibitory balance in neuronal circuits. This balance is significant for learning and memory processes. Neuroligin-3 through its interaction with neurexin also influences synaptic transmission by modulating the recruitment of signaling molecules at the synapse.

Abnormalities in NLGN3 are linked to neurological conditions such as autism spectrum disorders (ASD) and X-linked mental retardation. Mutations or dysregulation of NLGN3 affect synaptic function and are closely studied for their impact on neural communication in these conditions. The interaction of NLGN3 with neurexin and other neuroligins places it at the center of research focused on unraveling the pathophysiology of these disorders aiming to understand how disruption in synaptic adhesion molecules leads to cognitive and developmental challenges.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system.
See full target information Nlgn3
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB222806

Anti-Neurexin I beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-Neurexin I beta antibody (AB222806)

  • 0
  • 0
Primary Antibodies

AB308452

Anti-Neuroligin 1 antibody [EPR27213-94] - BSA and Azide free

RabMAb|Recombinant

Dot Blot - Anti-Neuroligin 1 antibody [EPR27213-94] - BSA and Azide free (AB308452)

  • 0
  • 0
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com