AB186307

Anti-Neuroligin 3 antibody [N110/29]

Name: Anti-Neuroligin 3 antibody [N110/29] (ab186307) | Abcam
Brand: Abcam Limited
SKU: AB186307

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(3 Publications)

Mouse Monoclonal Neuroligin 3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nlgn3 aa 700 to C-terminus.

View Alternative Names

KIAA1480, NL3, NLGN3, Neuroligin-3, Gliotactin homolog

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

4% formaldehyde-fixed SK-N-BE cells stained for Neuroligin 3 (green) using ab186307 at 1/100 dilution in ICC/IF.

Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at room temperature. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes room temperature, 5 minutes room temperature. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 3 Antibody. (D) Composite.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Neuroligin 3 with ab186307 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 3 Antibody (D) Composite.

Supplier Data

Western blot - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

All lanes:

Western blot - Anti-Neuroligin 3 antibody [N110/29] (ab186307) at 1/200 dilution

All lanes:

Mouse brain membrane lysate at 15 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/1000 dilution

Predicted band size: 94 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N110/29

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rat Nlgn3 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q62889

Specificity

ab186307 Does not cross-react with Neuroligin 1, 2, or 4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

The clone number has been updated from S110-29 to N110/29, both clone numbers name the same antibody clone.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuroligin-3 also known as NLGN3 is a synaptic cell adhesion molecule weighing approximately 83 kilodaltons. It is expressed in the central nervous system particularly in the brain where it plays a role in synapse formation and function. The protein facilitates the connection between neurons by interacting with neurexin another synaptic protein to form a complex that links pre- and postsynaptic neurons. This interaction is essential for establishing functional synapses.

Biological function summary

A role in the central nervous system highlights the importance of NLGN3 in the development and maintenance of synaptic networks. It is part of the neuroligin family which forms complexes with neurexins across the synaptic cleft to ensure proper communication between neurons. These complexes are important for synaptic health and function playing a role in neurotransmitter receptor clustering and signal transduction which affects synaptic plasticity and strength.

Pathways

NLGN3 contributes to the regulation of synaptic plasticity and the postsynaptic density assembly pathway. It interacts with proteins such as N110 and other neuroligins which are critical for the maintenance of excitatory and inhibitory balance in neuronal circuits. This balance is significant for learning and memory processes. Neuroligin-3 through its interaction with neurexin also influences synaptic transmission by modulating the recruitment of signaling molecules at the synapse.

Abnormalities in NLGN3 are linked to neurological conditions such as autism spectrum disorders (ASD) and X-linked mental retardation. Mutations or dysregulation of NLGN3 affect synaptic function and are closely studied for their impact on neural communication in these conditions. The interaction of NLGN3 with neurexin and other neuroligins places it at the center of research focused on unraveling the pathophysiology of these disorders aiming to understand how disruption in synaptic adhesion molecules leads to cognitive and developmental challenges.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and may mediate its effects by clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. May also play a role in glia-glia or glia-neuron interactions in the developing peripheral nervous system (By similarity).

See full target information NLGN3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15078 PubMed40301645

2025

Attenuated PINK1 autophosphorylation play neuroprotective and anti-seizure roles in neonatal hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Yuan,Xiaoqian Wang,Yaru Cui,Hua Zhou,Wenna Li,Qian Teng,Hongjin Wang,Bohan Sun,Qiaoyun Wang,Hongliu Sun,Jianhua Tang

Cell death & disease 14:423 PubMed37443071

2023

Targeting Wnt/β-catenin-mediated upregulation of oncogenic NLGN3 suppresses cancer stem cells in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jin Yun,Donghwi Kim,Sangwoo Kim,Jer-Tsong Hsieh,Seung Tae Baek

International journal of molecular sciences 21: PubMed32443651

2020

Prenatal Exposure to Valproic Acid Affects Microglia and Synaptic Ultrastructure in a Brain-Region-Specific Manner in Young-Adult Male Rats: Relevance to Autism Spectrum Disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Magdalena Gąssowska-Dobrowolska,Magdalena Cieślik,Grzegorz Arkadiusz Czapski,Henryk Jęśko,Małgorzata Frontczak-Baniewicz,Magdalena Gewartowska,Agnieszka Dominiak,Rafał Polowy,Robert Kuba Filipkowski,Lidia Babiec,Agata Adamczyk

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neuroligin 3 antibody [N110/29]

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

Immunocytochemistry/ Immunofluorescence - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

Western blot - Anti-Neuroligin 3 antibody [N110/29] (AB186307)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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