Rabbit Polyclonal Beta-3-tubulin antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TUBB3.
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- Stem cell reports 16:1276-12892021The role of SQSTM1 (p62) in mitochondrial function and clearance in human cortical neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Poon,Harpreet Saini,Siddharth Sethi,Gregory A O'Sullivan,Hélène Plun-Favreau,Selina Wray,Lee A Dawson,James M McCarthyPubMed 33891871
- Cellular and molecular neurobiology 38:1293-13032018Upregulation of miR-199a-5p Protects Spinal Cord Against Ischemia/Reperfusion-Induced Injury via Downregulation of ECE1 in Rat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Bao,Bo Fang,Huangwei Lv,Yanhua Jiang,Fengshou Chen,Zhilin Wang,Hong MaPubMed 29948551
- Brain research 1699:19-332018Transplantation of mesenchymal stem cells that overexpress NT-3 produce motor improvements without axonal regeneration following complete spinal cord transections in rats.Applications:IHC-PReactive species:Unspecified reactive speciesAndrew N Stewart et. al.PubMed 29883625
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TUBB3. The exact immunogen used to generate this antibody is proprietary information. Database link Q13509
Consider this alternative
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected | Expected |
| Mouse | Expected | Tested | Expected | Expected | Expected |
| Rat | Expected | Expected | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/50000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/50000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Alternative names
TUBB4, TUBB3, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III
Recommended products
Rabbit Polyclonal Beta-3-tubulin antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TUBB3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TUBB3. The exact immunogen used to generate this antibody is proprietary information. Database link Q13509
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuron-specific beta III tubulin also known as TUBB3 is a microtubule-associated protein with a molecular mass of approximately 50 kDa. This protein is expressed mostly in neurons but you may also find it in certain non-neuronal cells under specific conditions. As an important component of the cytoskeleton beta III tubulin collaborates with other tubulins to form microtubules essential for maintaining the structural integrity and transport functions within nerve cells.
- Biological function summary
Beta III tubulin maintains neuron stability and aids in processes like axonal guidance and dendritic growth. It does not usually form part of a larger complex but interacts dynamically with other microtubule-associated proteins to ensure proper neuron development and function. During neuronal differentiation beta III tubulin is often used as a marker due to its specific expression pattern in neurons compared to other tubulin isoforms.
- Pathways
Beta III tubulin plays an important role in the microtubule-based processes that are critical for the neurodevelopmental pathways. It interacts closely with proteins like Tau and MAP2 regulators of microtubule stability. These interactions fall within the larger scope of the axonal transport pathway a major pathway that involves other proteins such as kinesins and dyneins which drive cellular cargoes along the microtubules.
- Associated diseases and disorders
Beta III tubulin mutations relate to conditions like congenital fibrosis of the extraocular muscles (CFEOM) and neurological diseases. These mutations often disturb microtubule dynamics leading to impaired neuronal signaling and transport. The protein interacts with components like kinesin family motor proteins that contribute to axonal transport highlighting its significance in maintaining neural function and its potential in understanding neural-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin Western blot staining using rabbit Anti-Neuron specific beta III Tubulin antibody
All lanes: Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/5000 dilution
Lane 1: WT HeLa cell extract at 30 µg
Lane 2: Neuron specific beta III Tubulin Knockout HeLa cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 50 kDa
Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin Western blot staining using rabbit Anti-Neuron specific beta III Tubulin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/5000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: Neuron specific beta III Tubulin knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Immunohistochemistry (Frozen sections) - Anti-Neuron specific beta III Tubulin antibody (ab229590)
Frozen E13.5 rat brain tissue stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Neuron specific beta III Tubulin antibody
4 % paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Neuron specific beta III Tubulin antibody
Paraffin-embedded rat brain tissue stained for Neuron specific beta III Tubulin using ab229590 at 1/500 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229590. Western blot was performed from the immunoprecipitate using ab229590. Anti-rabbit IgG was used as a secondary antibody.
Lane 1: SK-N-SH whole cell extract (Input).
Lane 2: Control IgG instead of ab229590 in SK-N-SH whole cell extract.
Lane 3: ab229590 IP in SK-N-SH whole cell extract.All lanes: Immunoprecipitation - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Predicted band size: 50 kDa
Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin Western blot staining using rabbit Anti-Neuron specific beta III Tubulin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Lane 3: IMR32 whole cell extract at 30 µg
Lane 4: SK-N-AS whole cell extract at 30 µg
Predicted band size: 50 kDa
Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)
Neuron specific beta III Tubulin Western blot staining using rabbit Anti-Neuron specific beta III Tubulin antibody
10% SDS-PAGE gel.
All lanes: Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/50000 dilution
Lane 1: Mouse brain tissue extract at 50 µg
Lane 2: Rat brain tissue extract at 50 µg
Predicted band size: 50 kDa
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