Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker

• IP

Supplier Data

Immunoprecipitation - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

Neuron specific beta III Tubulin was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229590. Western blot was performed from the immunoprecipitate using ab229590. Anti-rabbit IgG was used as a secondary antibody.

Lane 1 : SK-N-SH whole cell extract (Input).
Lane 2 : Control IgG instead of ab229590 in SK-N-SH whole cell extract.
Lane 3 : ab229590 IP in SK-N-SH whole cell extract.

All lanes:

Immunoprecipitation - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590)

Predicted band size: 50 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

Paraffin-embedded rat brain tissue stained for Neuron specific beta III Tubulin using ab229590 at 1/500 dilution in immunohistochemical analysis.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

Frozen E13.5 rat brain tissue stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/250 dilution in immunohistochemical analysis.

Nuclear counterstain : Fluoroshield with DAPI (blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

4 % paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Fluoroshield with DAPI (blue).

• WB

Supplier Data

Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Lane 3:

IMR32 whole cell extract at 30 µg

Lane 4:

SK-N-AS whole cell extract at 30 µg

Predicted band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

All lanes:

Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/5000 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

Neuron specific beta III Tubulin Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (AB229590)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Neuron specific beta III Tubulin antibody - Neuronal Marker (ab229590) at 1/50000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Predicted band size: 50 kDa

false