Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)

Rabbit Recombinant Monoclonal Neuropeptide Y antibody. Carrier free. Suitable for mIHC, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Tested	Expected	Not recommended
Mouse	Tested	Tested	Tested	Not recommended
Rat	Tested	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes 1:2000 dilution recommended for human, mouse and rat species. 1:10000 dilution recommended for mouse adrenal gland tissue. Perform antigen retrieval with universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572)
Species Human	Dilution info -	Notes 1:2000 dilution recommended for human, mouse and rat species. 1:10000 dilution recommended for mouse adrenal gland tissue. Perform antigen retrieval with universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572)

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Associated Products

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Target data

See full target information NPY

Function

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

Alternative names

Pro-neuropeptide Y, NPY

Recommended products

Rabbit Recombinant Monoclonal Neuropeptide Y antibody. Carrier free. Suitable for mIHC, IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR21877
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab234527 is the carrier-free version of Anti-Neuropeptide Y antibody [EPR21877] ab221145.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neuropeptide Y (NPY) a 36 amino acid peptide functions mechanically as a neurotransmitter in the central and peripheral nervous systems. Alternate names include porcine peptide YY. The molecular weight of Neuropeptide Y is approximately 4 kDa. It expresses primarily in regions of the brain such as the hypothalamus and sympathetic nervous system as well as in other tissues including the heart adrenal glands and white adipose tissue.

Biological function summary

Neuropeptide Y plays an important role in regulating various physiological processes. It is not typically part of a complex but acts directly on specific receptors mainly Y1 Y2 Y4 and Y5. Neuropeptide Y affects food intake behavior stress response circadian rhythm and cardiovascular regulation. It does so by modulating the release of other neurotransmitters or hormones having a significant impact on energy balance and homeostasis.

Pathways

Neuropeptide Y plays a pivotal role in neurobiological signaling pathways especially those involving stress and energy homeostasis. In the hypothalamus NPY influences energy balance by interacting with the melanocortin pathway an important modulator of appetite and energy expenditure. It also interacts with proteins such as pro-opiomelanocortin (POMC) in these signaling pathways highlighting its integration into complex physiological processes that maintain homeostasis.

Associated diseases and disorders

Neuropeptide Y shows a significant connection to metabolic disorders and stress-related conditions. Dysregulation of NPY signaling has been implicated in obesity given its role in promoting food intake and energy storage. Additionally altered Neuropeptide Y activity links to anxiety and depression diseases involving stress-response dysregulation. Proteins like leptin which interacts with NPY in pathways governing hunger and energy allocation provide another layer of regulation affecting these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tisssue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive staining in hypothalamic paraventricular nucleus of rat cerebrum (PMID: 19357287) is observed. Counterstained with DAPI (Nuclear).
Perform heat mediated antigen retrieval with sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on mouse cerebrum (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on chromaffin cells of mouse adrenal gland medulla (PMID: 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Immunohistochemical analysis of paraffin-embedded human pheochromocytoma tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Cytoplasmic staining in human pheochromocytoma (PMID: 15623622) is obserevd. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572) was used for the antigen retreival.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Cytoplasmic staining in chromaffin cells of rat adrenal gland medulla (PMID: 15623622) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (Universal HIER antigen retrieval reagent (10X) ab208572) was used for the antigen retrieval.
Secondary antibody only control: used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (Anti-PCSK2 antibody [EPR23578-19] ab274418; gray; Opal™690) at 1:2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (Anti-CYP11A1 antibody [EPR24868-86] ab272494; green; Opal™520) at 1:10000 (0.053 μg/ml) [Panel C], and anti-Neuropeptide Y stained on chromaffin cells (Anti-Neuropeptide Y antibody [EPR21877] ab221145; red; Opal™570) at 1:2000 (0.279 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of Anti-PCSK2 antibody [EPR23578-19] ab274418, Anti-CYP11A1 antibody [EPR24868-86] ab272494, and Anti-Neuropeptide Y antibody [EPR21877] ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

This data was developed using Anti-Neuropeptide Y antibody [EPR21877] ab221145, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VGF antibody [EPR27029-60] ab313783).
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat adrenal gland staining of VGF with Anti-VGF antibody [EPR27029-60] ab313783 at a 1/500 (0.96 µg/ml) dilution, CYP11A1 with Anti-CYP11A1 antibody [EPR24868-86] ab272494 at a 1/10000 (0.053 µg/ml) dilution and Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at a 1/4000 (0.140 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-VGF (green; Opal™690), anti-CYP11A1 (magenta; Opal™520) and anti-Neuropeptide Y (gray; Opal™570) on rat adrenal gland.

Panel B: anti-VGF staining endocrine cells in rat adrenal gland medulla.

Panel C: anti-CYP11A1 staining adrenal cortex in rat adrenal gland.

Panel D: anti-Neuropeptide Y staining chromaffin cellsa in rat adrenal gland medulla.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-VGF antibody [EPR27029-60] ab313783, Anti-CYP11A1 antibody [EPR24868-86] ab272494 and Anti-Neuropeptide Y antibody [EPR21877] ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse adrenal gland staining of VGF with Anti-VGF antibody [EPR27029-60] ab313783 at a 1/500 (0.96 µg/ml) dilution, CYP11A1 with Anti-CYP11A1 antibody [EPR24868-86] ab272494 at a 1/10000 (0.053 µg/ml) dilution and Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at a 1/4000 (0.140 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-VGF (green; Opal™690), anti-CYP11A1 (magenta; Opal™520) and anti-Neuropeptide Y (gray; Opal™570) on mouse adrenal gland.

Panel B: anti-VGF staining endocrine cells in mouse adrenal gland medulla.

Panel C: anti-CYP11A1 staining adrenal cortex in mouse adrenal gland.

Panel D: anti-Neuropeptide Y staining endocrine cells in mouse adrenal gland medulla.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-VGF antibody [EPR27029-60] ab313783, Anti-CYP11A1 antibody [EPR24868-86] ab272494 and Anti-Neuropeptide Y antibody [EPR21877] ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Flow Cytometry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Neuropeptide Y antibody [EPR21877] ab221145).

Flow Cytometry analysis of Rat primary neural/glia cell labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/5000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control- Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left.
Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (ab234527)
This data was developed using Anti-Neuropeptide Y antibody [EPR21877] ab221145, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse adrenal gland staining of ADX with Anti-ADX antibody [EPR29804-54] ab321899 at a 1/2000 (0.244 µg/ml) dilution, VGF with Anti-VGF antibody [EPR27029-73] ab308287 at 1/500 (0.95 µg/ml) dilution and Neuropeptide Y with Anti-Neuropeptide Y antibody [EPR21877] ab221145 at 1/4000 ( 0.140 µg/ml) dilution.
Panel A: merged staining of anti-ADX (gray; Opal™690), anti-VGF (green; Opal™520) and anti-Neuropeptide Y (magenta; Opal™570) on mouse adrenal gland.

Panel B: anti-ADX staining adrenal cortex in mouse adrenal gland.

Panel C: anti-VGF staining endocrine cells in mouse adrenal gland medulla.

Panel D: nti-Neuropeptide Y staining chromaffin cells in mouse adrenal gland medulla.

Nuclear DNA was labelled with DAPI (shown in blue). ).
The section was incubated in three rounds of staining: in the order of Anti-ADX antibody [EPR29804-54] ab321899, Anti-VGF antibody [EPR27029-73] ab308287 and Anti-Neuropeptide Y antibody [EPR21877] ab221145 for 30 mins at room temperature.. Each round was followed by a separate fluorescent tyramide signal amplification system.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.