Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Fluorescence multiplex immunohistochemical analysis of human adrenal gland (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-PCSK2 stained on adrenal medulla (ab274418; gray; Opal™690) at 1 : 2000 (0.263 μg/ml) [Panel B], anti-CYP11A1 stained on adrenal cortex (ab272494; green; Opal™520) at 1 : 10000 (0.053 μg/ml) [Panel C], and anti-Neuropeptide Y stained on chromaffin cells (ab221145; red; Opal™570) at 1 : 2000 (0.279 μg/ml) [Panel D] on human adrenal gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab274418, ab272494, and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.  This data was developed using ab221145, the same antibody clone in a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Immunohistochemical analysis of paraffin-embedded human pheochromocytoma tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in human pheochromocytoma (PMID : 15623622) is obserevd. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retreival.
Secondary antibody only control : used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on mouse cerebrum (PMID : 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control : used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Neuropeptide Y with ab221145 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

This data was developed using ab221145, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse adrenal gland staining of ADX with ab321899 at a 1/2000 (0.244 µg/ml) dilution, VGF with ab308287 at 1/500 (0.95 µg/ml) dilution and Neuropeptide Y with ab221145 at 1/4000 ( 0.140 µg/ml) dilution.

Panel A : merged staining of anti-ADX (gray; Opal™690), anti-VGF (green; Opal™520) and anti-Neuropeptide Y (magenta; Opal™570) on mouse adrenal gland.
Panel B : anti-ADX staining adrenal cortex in mouse adrenal gland.
Panel C : anti-VGF staining endocrine cells in mouse adrenal gland medulla.
Panel D : nti-Neuropeptide Y staining chromaffin cells in mouse adrenal gland medulla.
Nuclear DNA was labelled with DAPI (shown in blue). ).

The section was incubated in three rounds of staining : in the order of ab321899, ab308287 and ab221145 for 30 mins at room temperature.. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on chromaffin cells of mouse adrenal gland medulla (PMID : 26444585) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control : used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse adrenal gland staining of VGF with ab313783 at a 1/500 (0.96 µg/ml) dilution, CYP11A1 with ab272494 at a 1/10000 (0.053 µg/ml) dilution and Neuropeptide Y with ab221145 at a 1/4000 (0.140 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-VGF (green; Opal™690), anti-CYP11A1 (magenta; Opal™520) and anti-Neuropeptide Y (gray; Opal™570) on mouse adrenal gland.
Panel B : anti-VGF staining endocrine cells in mouse adrenal gland medulla.
Panel C : anti-CYP11A1 staining adrenal cortex in mouse adrenal gland.
Panel D : anti-Neuropeptide Y staining endocrine cells in mouse adrenal gland medulla.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab313783, ab272494 and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab313783).

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat adrenal gland staining of VGF with ab313783 at a 1/500 (0.96 µg/ml) dilution, CYP11A1 with ab272494 at a 1/10000 (0.053 µg/ml) dilution and Neuropeptide Y with ab221145 at a 1/4000 (0.140 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-VGF (green; Opal™690), anti-CYP11A1 (magenta; Opal™520) and anti-Neuropeptide Y (gray; Opal™570) on rat adrenal gland.
Panel B : anti-VGF staining endocrine cells in rat adrenal gland medulla.
Panel C : anti-CYP11A1 staining adrenal cortex in rat adrenal gland.
Panel D : anti-Neuropeptide Y staining chromaffin cellsa in rat adrenal gland medulla.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab313783, ab272494 and ab221145 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Neuropeptide Y with ab221145 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Cytoplasmic staining in chromaffin cells of rat adrenal gland medulla (PMID : 15623622) is observed. Counterstained with hematoxylin. Universal HIER antigen retrieval reagent (10X) (ab208572) was used for the antigen retrieval.
Secondary antibody only control : used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).

• Flow Cyt

Lab

Flow Cytometry - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145). Flow Cytometry analysis of Rat primary neural/glia cell labeling Neuropeptide Y with ab221145 at 1/500 dilution followed by ab150081 at 1/5000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control- Rabbit monoclonal IgG (ab172730) / Left.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [EPR21877] - BSA and Azide free (AB234527)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat cerebrum tisssue labeling Neuropeptide Y with ab221145 at 1/100 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive staining in hypothalamic paraventricular nucleus of rat cerebrum (PMID : 19357287) is observed. Counterstained with DAPI (Nuclear).

Perform heat mediated antigen retrieval with sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab221145).