Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neuropeptide Y with ab281152 at 1/2000 (0.521 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum. The section was incubated with ab281152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Neuropeptide Y with ab281152 at 1/2000 (0.5 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).

Confocal image showing cytoplasmic staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : 293T.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1.0 µg/ml) dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) at a 1/1000 (2 µg/ml) dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuropeptide Y with ab281152 at 1/1000 (1.042 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum. The section was incubated with ab281152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Neuropeptide Y with ab281152 at 1/1000 (1.042 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Low expression : no staining on rat liver. The section was incubated with ab281152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuropeptide Y with ab281152 at 1/1000 (1.042 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum. The section was incubated with ab281152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuropeptide Y with ab281152 at 1/1000 (1.042 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : no staining on mouse liver. The section was incubated with ab281152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument
Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• sELISA

Lab

Sandwich ELISA - Anti-Neuropeptide Y antibody [EPR23483-18] - BSA and Azide free (Capture) (AB281152)

Sandwich ELISA of ab253592 with the capture antibody ab281152 dilution at 2 µg/mL and detector antibody ab281002 dilution at 0.5 µg/mL.