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AB281002

Anti-Neuropeptide Y antibody [EPR23483-22] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Neuropeptide Y antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

Pro-neuropeptide Y, NPY

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Sandwich ELISA - Anti-Neuropeptide Y antibody [EPR23483-22] - BSA and Azide free (Detector) (AB281002)
  • sELISA

Lab

Sandwich ELISA - Anti-Neuropeptide Y antibody [EPR23483-22] - BSA and Azide free (Detector) (AB281002)

Sandwich ELISA of ab253592 with the capture antibody ab281152 dilution at 2 µg/mL and detector antibody ab281002 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23483-22

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab281002 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human NPY. The recommended pair for sandwich ELISA is:
Capture: ab281152, Human NPY Capture Antibody (unconjugated)
Detector: ab281002, Human NPY Detector Antibody (unconjugated)
The reference range value is 0.39-50 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuropeptide Y (NPY) a 36 amino acid peptide functions mechanically as a neurotransmitter in the central and peripheral nervous systems. Alternate names include porcine peptide YY. The molecular weight of Neuropeptide Y is approximately 4 kDa. It expresses primarily in regions of the brain such as the hypothalamus and sympathetic nervous system as well as in other tissues including the heart adrenal glands and white adipose tissue.
Biological function summary

Neuropeptide Y plays an important role in regulating various physiological processes. It is not typically part of a complex but acts directly on specific receptors mainly Y1 Y2 Y4 and Y5. Neuropeptide Y affects food intake behavior stress response circadian rhythm and cardiovascular regulation. It does so by modulating the release of other neurotransmitters or hormones having a significant impact on energy balance and homeostasis.

Pathways

Neuropeptide Y plays a pivotal role in neurobiological signaling pathways especially those involving stress and energy homeostasis. In the hypothalamus NPY influences energy balance by interacting with the melanocortin pathway an important modulator of appetite and energy expenditure. It also interacts with proteins such as pro-opiomelanocortin (POMC) in these signaling pathways highlighting its integration into complex physiological processes that maintain homeostasis.

Neuropeptide Y shows a significant connection to metabolic disorders and stress-related conditions. Dysregulation of NPY signaling has been implicated in obesity given its role in promoting food intake and energy storage. Additionally altered Neuropeptide Y activity links to anxiety and depression diseases involving stress-response dysregulation. Proteins like leptin which interacts with NPY in pathways governing hunger and energy allocation provide another layer of regulation affecting these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
See full target information NPY
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com