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Rabbit Recombinant Multiclonal Neuropeptide Y antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (AB319116), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (AB319116), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (AB319116), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (AB319116), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (AB319116), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-PICC/IFFlow Cyt (Intra)WBIHC-Fr
Human
Tested
Tested
Tested
Not recommended
Not recommended
Expected
Mouse
Expected
Tested
Tested
Tested
Not recommended
Tested
Rat
Expected
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.

Alternative names

Recommended products

Rabbit Recombinant Multiclonal Neuropeptide Y antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
RM1201
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human FC

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab319116 is the carrier-free version of Anti-Neuropeptide Y antibody [RM1201] ab319115.

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Neuropeptide Y (NPY) a 36 amino acid peptide functions mechanically as a neurotransmitter in the central and peripheral nervous systems. Alternate names include porcine peptide YY. The molecular weight of Neuropeptide Y is approximately 4 kDa. It expresses primarily in regions of the brain such as the hypothalamus and sympathetic nervous system as well as in other tissues including the heart adrenal glands and white adipose tissue.

Biological function summary

Neuropeptide Y plays an important role in regulating various physiological processes. It is not typically part of a complex but acts directly on specific receptors mainly Y1 Y2 Y4 and Y5. Neuropeptide Y affects food intake behavior stress response circadian rhythm and cardiovascular regulation. It does so by modulating the release of other neurotransmitters or hormones having a significant impact on energy balance and homeostasis.

Pathways

Neuropeptide Y plays a pivotal role in neurobiological signaling pathways especially those involving stress and energy homeostasis. In the hypothalamus NPY influences energy balance by interacting with the melanocortin pathway an important modulator of appetite and energy expenditure. It also interacts with proteins such as pro-opiomelanocortin (POMC) in these signaling pathways highlighting its integration into complex physiological processes that maintain homeostasis.

Associated diseases and disorders

Neuropeptide Y shows a significant connection to metabolic disorders and stress-related conditions. Dysregulation of NPY signaling has been implicated in obesity given its role in promoting food intake and energy storage. Additionally altered Neuropeptide Y activity links to anxiety and depression diseases involving stress-response dysregulation. Proteins like leptin which interacts with NPY in pathways governing hunger and energy allocation provide another layer of regulation affecting these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

19 product images

  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat paraventricular nucleus tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)

    Confocal image showing positive staining on rat paraventricular nucleus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse paraventricular nucleus (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)

    Confocal image showing positive staining on mouse paraventricular nucleus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse arcuate nucleus (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)

    Confocal image showing positive staining on mouse arcuate nucleus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)

    Low expression: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)

    Low expression: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Negative control: 293T.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunoprecipitation - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunoprecipitation - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Neuropeptide Y was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lane 2: Anti-Neuropeptide Y antibody [RM1201] ab319115 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Neuropeptide Y antibody [RM1201] ab319115 in SH-SY5Y whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-Neuropeptide Y antibody [RM1201] (Anti-Neuropeptide Y antibody [RM1201] ab319115) at 1/30 dilution

    All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 119s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: no staining on rat liver. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: no staining on mouse liver. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/4000 (0.134 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: no staining on human liver. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat hypothalamus. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse hypothalamus. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebrum. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/4000 (0.134 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human cerebrum. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Neuropeptide Y antibody [RM1201] - BSA and Azide free (ab319116)

    This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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