Rabbit Recombinant Multiclonal Neuropeptide Y antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
IP | IHC-P | ICC/IF | Flow Cyt (Intra) | WB | IHC-Fr | |
---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Not recommended | Not recommended | Expected |
Mouse | Expected | Tested | Tested | Tested | Not recommended | Tested |
Rat | Expected | Tested | Tested | Tested | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
Pro-neuropeptide Y, NPY
Rabbit Recombinant Multiclonal Neuropeptide Y antibody. Carrier free. Suitable for IP, IHC-P, ICC/IF, Flow Cyt (Intra), IHC-Fr and reacts with Human, Mouse, Rat samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
Unsuitable for human FC
ab319116 is the carrier-free version of Anti-Neuropeptide Y antibody [RM1201] ab319115.
This product is a recombinant multiclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Neuropeptide Y (NPY) a 36 amino acid peptide functions mechanically as a neurotransmitter in the central and peripheral nervous systems. Alternate names include porcine peptide YY. The molecular weight of Neuropeptide Y is approximately 4 kDa. It expresses primarily in regions of the brain such as the hypothalamus and sympathetic nervous system as well as in other tissues including the heart adrenal glands and white adipose tissue.
Neuropeptide Y plays an important role in regulating various physiological processes. It is not typically part of a complex but acts directly on specific receptors mainly Y1 Y2 Y4 and Y5. Neuropeptide Y affects food intake behavior stress response circadian rhythm and cardiovascular regulation. It does so by modulating the release of other neurotransmitters or hormones having a significant impact on energy balance and homeostasis.
Neuropeptide Y plays a pivotal role in neurobiological signaling pathways especially those involving stress and energy homeostasis. In the hypothalamus NPY influences energy balance by interacting with the melanocortin pathway an important modulator of appetite and energy expenditure. It also interacts with proteins such as pro-opiomelanocortin (POMC) in these signaling pathways highlighting its integration into complex physiological processes that maintain homeostasis.
Neuropeptide Y shows a significant connection to metabolic disorders and stress-related conditions. Dysregulation of NPY signaling has been implicated in obesity given its role in promoting food intake and energy storage. Additionally altered Neuropeptide Y activity links to anxiety and depression diseases involving stress-response dysregulation. Proteins like leptin which interacts with NPY in pathways governing hunger and energy allocation provide another layer of regulation affecting these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat paraventricular nucleus tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)
Confocal image showing positive staining on rat paraventricular nucleus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse paraventricular nucleus (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)
Confocal image showing positive staining on mouse paraventricular nucleus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse arcuate nucleus (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)
Confocal image showing positive staining on mouse arcuate nucleus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)
Low expression: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (perfused fixed) tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at a 1/100 (5.36 µg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg/mL) dilution (Green)
Low expression: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 (0.536 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in SH-SY5Y cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control: 293T.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Neuropeptide Y was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2: Anti-Neuropeptide Y antibody [RM1201] ab319115 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Neuropeptide Y antibody [RM1201] ab319115 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
All lanes: Immunoprecipitation - Anti-Neuropeptide Y antibody [RM1201] (Anti-Neuropeptide Y antibody [RM1201] ab319115) at 1/30 dilution
All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 119s
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on rat liver. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on mouse liver. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/4000 (0.134 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on human liver. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat hypothalamus. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse hypothalamus. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/2000 (0.268 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse cerebrum. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/4000 (0.134 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with Anti-Neuropeptide Y antibody [RM1201] ab319115 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
This data was developed using Anti-Neuropeptide Y antibody [RM1201] ab319115, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neural/glia cell cells labelling Neuropeptide Y with Anti-Neuropeptide Y antibody [RM1201] ab319115 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
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