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Rabbit Recombinant Monoclonal Neurotensin antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (AB308302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (AB308302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (AB308302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (AB308302), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (AB308302), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Not recommended
Mouse
Tested
Not recommended
Rat
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Transfected cell line - Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell line - Human

Dilution info

-

Notes

-

Target data

Function

Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Neurotensin antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27180-62

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neurotensin also known by the abbreviation NT is a peptide neurotransmitter that consists of 13 amino acids giving it a relatively modest mass. It is well-known under the name NTS and is mainly expressed in the central nervous system especially within the hypothalamus midbrain and also found in peripheral tissues like the gastrointestinal tract. Neurotensin acts by binding to specific G-protein-coupled receptors such as NTSR1 to mediate its effects.

Biological function summary

The peptide neurotransmitter exhibits various functions in both the central and peripheral nervous systems. It can act as a neuromodulator affecting dopamine signaling and impacts the regulation of pain temperature and even food intake. Neurotensin forms part of complexes with other neurotransmitters and interacts closely with pathways related to dopamine suggesting a significant influence on mood and cognition.

Pathways

The interaction of neurotensin with dopamine pathways is particularly important. It plays a role in modulating the dopaminergic signaling cascades that involve receptor proteins like D2 dopamine receptors. Additionally neurotensin impacts the acute-phase response pathway altering the signaling dynamics in inflammation and immune response.

Associated diseases and disorders

Neurotensin has connections to conditions such as schizophrenia and obesity. Its modulation of dopamine pathways links it to schizophrenia where altered dopamine signaling is evident with potential involvement of proteins like NTSR1 and D2 receptors. Additionally because neurotensin influences food intake and energy balance it relates to obesity with its effects mediated through interactions with other gut-derived peptides and receptors including ghrelin and leptin receptors.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Negative control: no staining in human skeletal muscle.

    The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Negative control: no staining in mouse skeletal muscle.

    The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302)

    Immunohistochemical analysis of paraffin-embedded cells labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Positive staining on (A) HEK-293T transfected with a human NTS expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag.

    The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.531 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining in a subset of cells on mouse colon (PMID:26469136).

    The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Positive staining in a subset of cells on mouse small intestine. The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] (ab308302)

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.531 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Positive staining in a subset of cells on human duodenum (PMID:23064014).

    The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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