Rabbit Recombinant Monoclonal Neurotensin antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human, Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | |
---|---|---|
Human | Tested | Not recommended |
Mouse | Tested | Not recommended |
Rat | Not recommended | Not recommended |
Transfected cell line - Human | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.
Neurotensin/neuromedin N, NTS
Rabbit Recombinant Monoclonal Neurotensin antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human, Mouse samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR27180-62
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Neurotensin also known by the abbreviation NT is a peptide neurotransmitter that consists of 13 amino acids giving it a relatively modest mass. It is well-known under the name NTS and is mainly expressed in the central nervous system especially within the hypothalamus midbrain and also found in peripheral tissues like the gastrointestinal tract. Neurotensin acts by binding to specific G-protein-coupled receptors such as NTSR1 to mediate its effects.
The peptide neurotransmitter exhibits various functions in both the central and peripheral nervous systems. It can act as a neuromodulator affecting dopamine signaling and impacts the regulation of pain temperature and even food intake. Neurotensin forms part of complexes with other neurotransmitters and interacts closely with pathways related to dopamine suggesting a significant influence on mood and cognition.
The interaction of neurotensin with dopamine pathways is particularly important. It plays a role in modulating the dopaminergic signaling cascades that involve receptor proteins like D2 dopamine receptors. Additionally neurotensin impacts the acute-phase response pathway altering the signaling dynamics in inflammation and immune response.
Neurotensin has connections to conditions such as schizophrenia and obesity. Its modulation of dopamine pathways links it to schizophrenia where altered dopamine signaling is evident with potential involvement of proteins like NTSR1 and D2 receptors. Additionally because neurotensin influences food intake and energy balance it relates to obesity with its effects mediated through interactions with other gut-derived peptides and receptors including ghrelin and leptin receptors.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining in human skeletal muscle.
The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Negative control: no staining in mouse skeletal muscle.
The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded cells labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining on (A) HEK-293T transfected with a human NTS expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag.
The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.531 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining in a subset of cells on mouse colon (PMID:26469136).
The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Positive staining in a subset of cells on mouse small intestine. The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.531 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Positive staining in a subset of cells on human duodenum (PMID:23064014).
The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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