AB308303

Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Neurotensin antibody. Carrier free. Suitable for IHC-P and reacts with Human, Transfected cell line - Human, Mouse samples.

View Alternative Names

Neurotensin/neuromedin N, NTS

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

This data was produced using ab308302, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded cells labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on (A) HEK-293T transfected with a human NTS expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

This data was produced using ab308302, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.531 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in a subset of cells on human duodenum (PMID : 23064014). The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

This data was produced using ab308302, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining in human skeletal muscle. The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

This data was produced using ab308302, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining in mouse skeletal muscle. The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

This data was produced using ab308302, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.531 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining in a subset of cells on mouse colon (PMID : 26469136). The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

This data was produced using ab308302, the same clone but in a different formulation. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling Neurotensin with ab308302 at 1/2000 dilution (0.266 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in a subset of cells on mouse small intestine. The section was incubated with ab308302 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Unconjugated

Anti-Neurotensin antibody [EPR27180-62]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27180-62

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurotensin also known by the abbreviation NT is a peptide neurotransmitter that consists of 13 amino acids giving it a relatively modest mass. It is well-known under the name NTS and is mainly expressed in the central nervous system especially within the hypothalamus midbrain and also found in peripheral tissues like the gastrointestinal tract. Neurotensin acts by binding to specific G-protein-coupled receptors such as NTSR1 to mediate its effects.

Biological function summary

The peptide neurotransmitter exhibits various functions in both the central and peripheral nervous systems. It can act as a neuromodulator affecting dopamine signaling and impacts the regulation of pain temperature and even food intake. Neurotensin forms part of complexes with other neurotransmitters and interacts closely with pathways related to dopamine suggesting a significant influence on mood and cognition.

Pathways

The interaction of neurotensin with dopamine pathways is particularly important. It plays a role in modulating the dopaminergic signaling cascades that involve receptor proteins like D2 dopamine receptors. Additionally neurotensin impacts the acute-phase response pathway altering the signaling dynamics in inflammation and immune response.

Neurotensin has connections to conditions such as schizophrenia and obesity. Its modulation of dopamine pathways links it to schizophrenia where altered dopamine signaling is evident with potential involvement of proteins like NTSR1 and D2 receptors. Additionally because neurotensin influences food intake and energy balance it relates to obesity with its effects mediated through interactions with other gut-derived peptides and receptors including ghrelin and leptin receptors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.

See full target information NTS

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurotensin antibody [EPR27180-62] - BSA and Azide free (AB308303)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise