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AB274417

Anti-Neurturin antibody [EPR23301-10]

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(3 Publications)

Rabbit Recombinant Monoclonal Neurturin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Neurturin, NRTN

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID : 25841760). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis. The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurturin antibody [EPR23301-10] (AB274417)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Neurturin with ab274417 at 1/500 (1.094 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID : 10415380). The section was incubated with ab274417 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Western blot - Anti-Neurturin antibody [EPR23301-10] (AB274417)
  • WB

Lab

Western blot - Anti-Neurturin antibody [EPR23301-10] (AB274417)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 15829225, PMID : 24067900).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

Human brain tissue lysate at 20 µg

Lane 4:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

VeriBlot for IP secondary antibody(HRP)(<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 16 kDa,28 kDa

false

Western blot - Anti-Neurturin antibody [EPR23301-10] (AB274417)
  • WB

Lab

Western blot - Anti-Neurturin antibody [EPR23301-10] (AB274417)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1-3 were developed using a higher sensitivity ECL substrate.

16-kDa Monomeric and 28-kDa dimeric form of Neurturin are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 15829225, PMID : 24067900).

Exposure times : Lane 1-3 : 3 minutes Lane 4-5 : 37 seconds.

All lanes:

Western blot - Anti-Neurturin antibody [EPR23301-10] (ab274417) at 1/1000 dilution

Lane 1:

Mouse embryo tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 4:

Rat heart tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa

Observed band size: 16 kDa,28 kDa

false

  • Carrier free

    Anti-Neurturin antibody [EPR23301-10] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23301-10

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neurturin also called NRTN is a neurotrophic factor belonging to the glial cell line-derived neurotrophic factor (GDNF) family. It is a protein weighing approximately 23 kDa. Neurturin promotes the survival of neurons and is mainly active in the central nervous system and peripheral nervous system. It is expressed in various tissues including the brain spinal cord and organs such as the liver and pancreas.
Biological function summary

Neurturin supports the maintenance and function of many neuronal populations. It partners with the RET receptor tyrosine kinase and the GFRα2 co-receptor to form a receptor complex that mediates its signaling. This interaction is important for neuronal differentiation and survival especially in dopamine-producing neurons and parasympathetic neurons. Neurturin's activity ensures proper nerve development and the maintenance of effective neuronal networks.

Pathways

Several important elements are involved with neurturin function. It plays a role in the MAPK/ERK and the PI3K/Akt signaling pathways both of which are essential for cell survival and growth. In these pathways neurturin acts alongside proteins like GDNF and RET helping to transmit cellular signals that either promote cell survival or modulate neuronal function depending upon the cellular context.

Research links neurturin to conditions like Parkinson’s disease and Hirschsprung's disease. In Parkinson’s disease the decrease in dopaminergic neurons correlates with malfunction or decreased activity of neurturin and its associated receptors like RET. Similarly insufficient signaling involving neurturin and its receptor complex might contribute to the development of Hirschsprung's disease a disorder related to the absence of nerve cells in the colon.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Growth factor that supports the survival of sympathetic neurons in culture (PubMed : 8945474). May regulate the development and maintenance of the CNS (PubMed : 8945474). Involved in the development of the neural crest (PubMed : 15242795). Might control the size of non-neuronal cell population such as haemopoietic cells (PubMed : 8945474). Acts by binding to its coreceptor, GFRA2, leading to autophosphorylation and activation of the RET receptor (PubMed : 10829012, PubMed : 29414779, PubMed : 31535977). Heparan sulfate-binding is required for signaling (PubMed : 29414779).
See full target information NRTN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 20:571 PubMed40474233

2025

Sympathetic cholinergic nerve fibres promote BMSC differentiation into osteoblasts via NRTN secretion: a potential treatment for osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuechun Chen,Zhenyu Zhang,Huibo Ti,Junjie Wu,Feng-Lai Yuan,Xia Li

eLife 12: PubMed36810133

2023

Enteroendocrine cell lineages that differentially control feeding and gut motility.

Applications

Unspecified application

Species

Unspecified reactive species

Marito Hayashi,Judith A Kaye,Ella R Douglas,Narendra R Joshi,Fiona M Gribble,Frank Reimann,Stephen D Liberles

Cell stem cell 29:528-544.e9 PubMed35276096

2022

A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen Gadomski,Claire Fielding,Andrés García-García,Claudia Korn,Chrysa Kapeni,Sadaf Ashraf,Javier Villadiego,Raquel Del Toro,Olivia Domingues,Jeremy N Skepper,Tatiana Michel,Jacques Zimmer,Regine Sendtner,Scott Dillon,Kenneth E S Poole,Gill Holdsworth,Michael Sendtner,Juan J Toledo-Aral,Cosimo De Bari,Andrew W McCaskie,Pamela G Robey,Simón Méndez-Ferrer
View all publications
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