Anti-Neutrophil antibody [7/4]

• Flow Cyt

Unknown

Flow Cytometry - Anti-Neutrophil antibody [7/4] (AB53457)

Staining of mouse peripheral blood granulocytes with Rat anti mouse neutrophil (ab53457).

• IHC

CiteAb

Immunohistochemistry - Anti-Neutrophil antibody [7/4] (AB53457)

Immunohistochemistry-immunofluorescence using Anti-Neutrophil antibody [7/4]Anti-Neutrophil antibody [7/4], ab53457. Publication image from Zhao, X. et al., 2017, Nat Commun, 28928459. Legend direct from paper.

IL-27 induces LTF in PMNs. a Photomicrograph of representative LTF, immunostaining (green) in normal mouse BM-PMNs. The nuclei are stained with DAPI (red). b, c MFI of LTF, staining per cell was determined in BM-PMNs by flow cytometry, with T cells and monocytes serving as negative controls. Analyzed cells were from mice treated with saline or rIL-27 (50 ng/kg, i.v., at 30 min, 24 h, 42 h after ICH) and harvested at 48 h after the ICH. d Photomicrograph of representative LTF staining (green) in peripheral blood mature PMNs. The nuclei were stained with DAPI. Scale bar = 10 µM. e Bar graph of LTF, protein content (established with ELISA) in peripheral blood PMNs in naïve rats, and rats 48 h after ICH with and without treatment with rIL-27 (50 ng/kg, i.v., at 30 min, 24 h, 42 h after ICH). Data are expressed as mean ± SEM (n = 5). *p ≤ 0.05, vs. naïve rat, **p ≤ 0.05, vs. naïve rats and rats after ICH without IL-27 treatment. The p-value was established using one-way ANOVA followed by Newman–Keuls post-test. f Immunofluorescence of LTF, (red) and neutrophil elastase, ELANE (green) in a mouse brain at hematoma-affected area at 24 h after ICH. The nuclei were labeled with DAPI (blue). Scale bar = 50 µm. The insertion window highlighted the co-localization of LTF, with ELANE+ neutrophil. g LTF, protein in the ICH-affected rat brains (harvested from animals perfused with saline to flash intravascular cells), in the same animals as in e, and established using Western blot (n = 5)