Rabbit Polyclonal Neutrophil Bactericidal Protein BP30 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human BPI.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Expected | Tested |
Mouse | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Bactericidal permeability-increasing protein, BPI, CAP 57, BPI
Rabbit Polyclonal Neutrophil Bactericidal Protein BP30 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human BPI.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Neutrophil Bactericidal Protein BP30 also known as NGAL or LCN2 is a small protein with a mass of approximately 25 kDa. It is expressed chiefly in neutrophils but is also found in several other tissues including the liver lung and kidney. Mechanically BP30 plays a role in binding and transporting iron by sequestering bacterial siderophores which deprives bacteria of the iron essential for their growth. This activity is important for its function in the immune response.
BP30 exerts antimicrobial effects by interfering with bacterial iron acquisition limiting bacterial growth and proliferation. It is not part of a complex; rather it functions as a monomer although it can form complexes with siderophores. Beyond its antimicrobial role BP30 also participates in cellular processes like apoptosis and inflammation. Its presence in various tissues points to its function as more systemically involved in defense mechanisms.
BP30 interacts with several important biological pathways including the innate immune response and iron metabolism. Within the innate immune pathway it works alongside other antimicrobial peptides and proteins to enhance the body's first line of defense against pathogens. Additionally in the iron metabolism pathway BP30 modulates iron availability interacting with proteins such as transferrin and ferritin to balance iron homeostasis.
BP30 has a notable connection with kidney disease and some bacterial infections. In kidney disease BP30 serves as a biomarker for acute kidney injury indicating elevated levels when the kidneys are damaged. Its link to bacterial infections results from its primary function of iron sequestration depriving bacteria of necessary nutrients. BP30 is connected to hepcidin through its role in iron regulation which can influence the progression of these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-Neutrophil Bactericidal Protein BP30 antibody (ab196732) at 1/500 dilution
Lane 1: Jurkat cell extract
Lane 2: HepG2 cell extract
Lane 3: SK-Hep1 cell extract
Lane 4: Mouse liver extract
Lane 5: Mouse thymus extract
Predicted band size: 54 kDa
Immunohistochemical analysis of paraffin-embedded Mouse liver cancer tissue labeling Neutrophil Bactericidal Protein BP30 with ab196732 at 1/200 dilution.
Immunofluorescent analysis of A549 cells labeling Neutrophil Bactericidal Protein BP30 with ab196732 at 1/50 dilution.
Blue: DAPI for nuclear staining.
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