Anti-Neutrophil Elastase rabbit polyclonal antibody that is used to detect Neutrophil Elastase in Western blot, IHC. Suitable for Human, Mouse samples.
- Cited in over 215 publications
- Trusted since 2009
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- Nature metabolism 5:2111-21302023Iron accumulation drives fibrosis, senescence and the senescence-associated secretory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMate Maus,Vanessa López-Polo,Lidia Mateo,Miguel Lafarga,Mònica Aguilera,Eugenia De Lama,Kathleen Meyer,Anna Sola,Cecilia Lopez-Martinez,Ines López-Alonso,Marc Guasch-Piqueras,Fernanda Hernandez-Gonzalez,Selim Chaib,Miguel Rovira,Mayka Sanchez,Rosa Faner,Alvar Agusti,Rodrigo Diéguez-Hurtado,Sagrario Ortega,Anna Manonelles,Stefan Engelhardt,Freddy Monteiro,Camille Stephan-Otto Attolini,Neus Prats,Guillermo Albaiceta,Josep M Cruzado,Manuel SerranoPubMed 38097808
- Veterinary research 54:1052023Fasciola hepatica soluble antigens (FhAg) induce ovine PMN innate immune reactions and NET formation in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamara Muñoz-Caro,Marcela Gómez-Ceruti,Liliana M R Silva,Daniel Gutiérrez-Expósito,Henrik Wagner,Anja Taubert,Carlos HermosillaPubMed 37953317
- iScience 26:1082892023Neutrophil extracellular traps and DNases orchestrate formation of peritoneal adhesions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulia Elrod,Annika Heuer,Jasmin Knopf,Janina Schoen,Lavinia Schönfeld,Magdalena Trochimiuk,Carolin Stiel,Birgit Appl,Laia Pagerols Raluy,Ceren Saygi,Leticija Zlatar,Sami Hosari,Dmytro Royzman,Thomas H Winkler,Günter Lochnit,Moritz Leppkes,Robert Grützmann,Georg Schett,Christian Tomuschat,Konrad Reinshagen,Martin Herrmann,Tobias A Fuchs,Michael BoettcherPubMed 38034352
- FEMS microbes 4:xtad0192023suppresses -induced NETosis and promotes bacterial survival in polymicrobial infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick Hsien-Neng Kao,Jun-Hong Ch'ng,Kelvin K L Chong,Claudia J Stocks,Siu Ling Wong,Kimberly A KlinePubMed 37900578
- Frontiers in immunology 14:12440682023-induced neutrophil clustering and neutrophil extracellular trap formation depend on P2X1 purinergic receptor signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriel Espinosa,Iván Conejeros,Lisbeth Rojas-Barón,Carlos Rodrigo Hermosilla,Anja TaubertPubMed 37854595
- EJNMMI radiopharmacy and chemistry 8:232023Imaging of fibrogenesis in the liver by [F]TZ-Z09591, an Affibody molecule targeting platelet derived growth factor receptor β.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOlivia Wegrzyniak,Bo Zhang,Johanna Rokka,Maria Rosestedt,Bogdan Mitran,Pierre Cheung,Emmi Puuvuori,Sofie Ingvast,Jonas Persson,Helena Nordström,John Löfblom,Fredrik Pontén,Fredrik Y Frejd,Olle Korsgren,Jonas Eriksson,Olof ErikssonPubMed 37733133
- Frontiers in veterinary science 10:12567262023-induced neutrophil extracellular traps in neonatal calves is a stage-independent process.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Grabbe,Iván Conejeros,Zahady D Velásquez,Seyed Sajjad Hasheminasab,Faustin Kamena,Axel Wehrend,Ulrich Gärtner,Anja Taubert,Carlos Rodrigo HermosillaPubMed 37662980
- Parasite immunology 45:e130012023Toxoplasma gondii triggers heterophil extracellular traps via NADPH oxidase, ERK and P38 signalling pathways, glycolysis and autophagy in chickens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiyi Chen,Zha Jin,Qinqin Jin,Wei Liu,Xinxin Gao,Hongrong Hong,Yuxiao Qian,Yuqian Jiang,Quan Liu,Zhengkai WeiPubMed 37340931
- Frontiers in veterinary science 10:11761442023Induction of NETosis in ovine colostral PMN upon exposure to tachyzoites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLukas Demattio,Ivan Conejeros,Daniela Grob,Ulrich Gärtner,Anja Taubert,Carlos Hermosilla,Axel WehrendPubMed 37404777
- The Journal of dermatology 50:1150-11552023Three cases of subcorneal pustular dermatosis with immunohistochemical examinations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegumi Kishimoto,Mayumi Komine,Hirofumi Okada,Atsuko Sato,Koji Kamiya,Takeo Maekawa,Satoru Murata,Mamitaro OhtsukiPubMed 37288510
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Not recommended |
| Mouse | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/200 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes In our hands, when tested in western blot, this product typically gives a weaker signal in human tissue lysates compared to human cell lines and mouse lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. |
Species Human | Dilution info - | Notes In our hands, when tested in western blot, this product typically gives a weaker signal in human tissue lysates compared to human cell lines and mouse lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. |
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Target data
Function
Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed:25211214, PubMed:9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed:20676107, PubMed:25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).
Alternative names
ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE
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Anti-Neutrophil Elastase rabbit polyclonal antibody that is used to detect Neutrophil Elastase in Western blot, IHC. Suitable for Human, Mouse samples.
- Cited in over 215 publications
- Trusted since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
We have had mixed results for use of this antibody in IHC-P using Mouse tissue samples. The abReview data below from external researchers reports good staining with this antibody in Mouse tissue samples using IHC-Fr.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Neutrophil Elastase antibody (ab68672) is a rabbit polyclonal antibody and is validated for use in IHC-P in human, mouse samples.
Anti-Neutrophil Elastase antibody (ab68672) specifically detects Neutrophil Elastase (UniProt ID: P08246; Molecular weight: 26kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Neutrophil Elastase antibody (ab68672) has high sensitivity and specificity.
Anti-Neutrophil Elastase antibody (ab68672) has been cited over 220 times in peer reviewed journals and is trusted by the scientific community.
Anti-Neutrophil Elastase antibody (ab68672) has 18 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab68672)
Immunohistochemistical detection of Neutrophil Elastase using antibody (ab68672) on formaldehyde-fixed paraffin-embedded human spleen sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Permeabilization: No Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody dilution 1/2000; incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG conjugated to biotin (1/200).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab68672)
IHC image of Neutrophil Elastase staining in human spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68672, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-Neutrophil Elastase antibody (ab68672)
All lanes: Western blot - Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/mL
All lanes: Western blot - Native human Neutrophil Elastase protein (Active) (Native human Neutrophil Elastase protein (Active) ab91099) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab68672)
Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling Neutrophil Elastase with ab68672 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
Anti-Neutrophil Elastase antibody was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab68672)
IHC image of Neutrophil Elastase staining in a section of formalin-fixed paraffin-embedded normal mouse spleen performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab68672, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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