Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)

Rabbit Recombinant Monoclonal Neutrophil Elastase antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, mIHC and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (AB310335), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	Flow Cyt (Intra)	IHC-P	ICC/IF	IP	mIHC
Human	Tested	Expected	Tested	Not recommended	Not recommended	Expected
Mouse	Tested	Tested	Tested	Not recommended	Not recommended	Tested
Rat	Tested	Expected	Tested	Not recommended	Not recommended	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes ab310335 is not suitable for detecting human cell lines in western blot assay due to low sensitivity issue. Anti-Neutrophil Elastase antibody [EPR7479] ab131260 and Anti-Neutrophil Elastase antibody [RM1077] ab314916 are alternatives for detecting human cell lines.
Species Mouse	Dilution info 1/1000	Notes 0.538 ug/ml
Species Rat	Dilution info 1/1000	Notes 0.538 ug/ml

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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Target data

See full target information Elane

Function

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (By similarity). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (By similarity). Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli (PubMed:10947984).

Alternative names

Ela2, Elane, Neutrophil elastase, Elastase-2, Leukocyte elastase

Recommended products

Rabbit Recombinant Monoclonal Neutrophil Elastase antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, mIHC and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28386-66
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.

Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Associated diseases and disorders

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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22 product images

Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Neutrophil Elastase Western blot staining using rabbit Anti-Neutrophil Elastase antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab310335 is not suitable for detecting human cell lines in western blot assay. Anti-Neutrophil Elastase antibody [EPR7479] ab131260 and Anti-Neutrophil Elastase antibody [RM1077] ab314916 are alternatives for detecting human cell lines.
Neutrophil elastase undergoes proteolytic cleavage to generate fragments (PMID: 25857284).
Lanes 1 - 3: Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335) at 1/1000 dilution
Lanes 4 - 6: Western blot - Anti-Neutrophil Elastase antibody [EPR7479] (Anti-Neutrophil Elastase antibody [EPR7479] ab131260) at 1/1000 dilution
Lanes 7 - 9: Western blot - Anti-Neutrophil Elastase antibody [RM1077] (Anti-Neutrophil Elastase antibody [RM1077] ab314916) at 1/1000 dilution
Lanes 1, 4 and 7: HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 20 µg
Lanes 2, 5 and 8: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lanes 3, 6 and 9: U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 30 kDa, 26 kDa
Exposure time: 40s
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse bone marrow tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative controls: brain (PMID: 24309898), kidney (PMID: 24309898), cerebellum (PMID: 24309898).

In Western blot, anti- Vinculin antibody (Anti-Vinculin antibody [EPR8185] ab129002) loading control staining at 1/10000 dilution.
Exposure time: 48 seconds
All lanes: Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335) at 1/1000 dilution
Lane 1: Mouse bone marrow tissue lysate at 20 µg
Lane 2: Mouse cerebellum tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse brain tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 29 kDa
Exposure time: 48s
Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: brain (PMID: 24309898), cerebellum (PMID: 24309898).

In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 48 seconds
All lanes: Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335) at 1/1000 dilution
Lane 1: Human bone Marrow tissue lysate at 50 µg
Lane 2: Human brain tissue lysate at 20 µg
Lane 3: Human cerebellum tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 29 kDa
Exposure time: 48s
Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative controls: kidney (PMID: 24309898), cerebellum (PMID: 24309898).

In Western blot, anti- Integrin alpha V antibody (Anti-Integrin alpha V antibody [EPR16800] ab179475) loading control staining at 1/5000 dilution.
Exposure time: 180 seconds
All lanes: Western blot - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335) at 1/1000 dilution
Lane 1: Rat bone marrow tissue lysate at 20 µg
Lane 2: Rat cerebellum tissue lysate at 20 µg
Lane 3: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Exposure time: 180s
Flow Cytometry (Intracellular) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized mouse primary neuron cells labelling Neutrophil Elastase with ab310335 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Neutrophil Elastase with ab310335 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse bone marrow cells labelling Neutrophil Elastase with ab310335 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Neutrophil Elastase with ab310335 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on rat cerebrum. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neutrophil Elastase with ab310335 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on human cerebrum. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Neutrophil Elastase with ab310335 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse cerebrum. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Neutrophil Elastase with ab310335 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on neutrophils in rat spleen. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Human cervical cance tissue labeling Neutrophil Elastase with ab310335 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on neutrophils in human cervical cancer. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Neutrophil Elastase with ab310335 at 1/100 (5.38 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on neutrophils in human spleen. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic can tissue labeling Neutrophil Elastase with ab310335 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on neutrophils in mouse pancreatic cancer. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody [EPR28386-66] (ab310335)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Neutrophil Elastase with ab310335 at 1/500 (1.076 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on neutrophils in mouse spleen. The section was incubated with ab310335 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins