AB254178

Anti-Neutrophil Elastase antibody [NP57]

Recombinant
What is this?

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(13 Publications)

Anti-Neutrophil Elastase antibody [NP57] (ab254178) is a mouse monoclonal antibody detecting Neutrophil Elastase in ICC/IF. Suitable for Human.

View Alternative Names

ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (AB254178)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (AB254178)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labeling Neutrophil Elastase with ab254178 at 11.16μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on U-937 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab254178 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

Negative control : Jurkat（PMID : 22564522.

Carrier free

Anti-Neutrophil Elastase antibody [NP57] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NP57

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Neutrophil Elastase antibody [NP57] (ab254178) is a mouse recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Other related products
We have a range of other formats of antibody clone [NP57] also available for your convenience: ab254178, Carrier free - ab255935

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.

Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed : 15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed : 36899106). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed : 25211214, PubMed : 9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed : 20676107, PubMed : 25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed : 10947984).

See full target information ELANE

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111925 PubMed40034857

2025

Gasdermin D-dependent neutrophil extracellular traps exacerbate cytokine storm contributing to pyoderma gangrenosum pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Li,Shuni Ying,Hong Fang,Jianjun Qiao

mBio 16:e0327424 PubMed39679679

2024

Inhibition and evasion of neutrophil microbicidal responses by .

Applications

Unspecified application

Species

Unspecified reactive species

Hannah E Hanford,Christopher T D Price,Silvia Uriarte,Yousef Abu Kwaik

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2401195 PubMed38582501

2024

Innovative Bio-based Hydrogel Microspheres Micro-Cage for Neutrophil Extracellular Traps Scavenging in Diabetic Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Xiao,Tao Ding,He Fang,Jiawei Lin,Lili Chen,Duan Ma,Tianyu Zhang,Wenguo Cui,Jing Ma

Frontiers in immunology 13:1046778 PubMed36569875

2022

Increased formation of neutrophil extracellular traps in patients with anti-N-methyl-d-aspartate receptor encephalitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Qiao,Quan-Ye Sun,Peng Zhou,Shan-Chao Zhang,Zhi-Hao Wang,Hai-Yun Li,Ai-Hua Wang,Xue-Wu Liu,Tao Xin

Frontiers in oncology 12:850363 PubMed36249068

2022

Serum-derived piR-hsa-164586 of extracellular vesicles as a novel biomarker for early diagnosis of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanli Li,Yanhan Dong,Shupeng Zhao,Jinning Gao,Xiaodan Hao,Zibo Wang,Meng Li,Mengyuan Wang,Yiming Liu,Xiaoling Yu,Wenhua Xu

Frontiers in immunology 13:827146 PubMed35320941

2022

SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Perico,Marina Morigi,Miriam Galbusera,Anna Pezzotta,Sara Gastoldi,Barbara Imberti,Annalisa Perna,Piero Ruggenenti,Roberta Donadelli,Ariela Benigni,Giuseppe Remuzzi

The British journal of dermatology 175:122-33 PubMed26853903

2016

IgE and FcεRI are highly expressed on innate cells in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

K-X Yan,Q Huang,X Fang,Z-H Zhang,L Han,K Gadaldi,K-F Kang,Z-Z Zheng,J-H Xu,N Yawalkar

Scientific reports 6:21288 PubMed26887258

2016

Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Snäll,Anna Linnér,Julia Uhlmann,Nikolai Siemens,Heike Ibold,Marton Janos,Adam Linder,Bernd Kreikemeyer,Heiko Herwald,Linda Johansson,Anna Norrby-Teglund

mBio 6:e01390-15 PubMed26396242

2015

Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Hochman,Theresa F Madaline,Samuel C Wassmer,Emmie Mbale,Namjong Choi,Karl B Seydel,Richard O Whitten,Julie Varughese,Georges E R Grau,Steve Kamiza,Malcolm E Molyneux,Terrie E Taylor,Sunhee Lee,Danny A Milner,Kami Kim

The American journal of clinical nutrition 102:608-15 PubMed26178731

2015

A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mark D Farrar,Anna Nicolaou,Kayleigh A Clarke,Sarah Mason,Karen A Massey,Tristan P Dew,Rachel E B Watson,Gary Williamson,Lesley E Rhodes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neutrophil Elastase antibody [NP57]

Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (AB254178)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise