Mouse Recombinant Monoclonal Neutrophil Elastase antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 9 publications.
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![Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (AB254178), expandable thumbnail](https://content.abcam.com/products/images/neutrophil-elastase-antibody-np57-ab254178--immunocytochemistry-immunofluorescence-img22558.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (AB254178)")
Publications
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- Frontiers in immunology 13:10467782022Increased formation of neutrophil extracellular traps in patients with anti-N-methyl-d-aspartate receptor encephalitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShan Qiao,Quan-Ye Sun,Peng Zhou,Shan-Chao Zhang,Zhi-Hao Wang,Hai-Yun Li,Ai-Hua Wang,Xue-Wu Liu,Tao XinPubMed 36569875
- Frontiers in immunology 13:8271462022SARS-CoV-2 Spike Protein 1 Activates Microvascular Endothelial Cells and Complement System Leading to Platelet Aggregation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuca Perico,Marina Morigi,Miriam Galbusera,Anna Pezzotta,Sara Gastoldi,Barbara Imberti,Annalisa Perna,Piero Ruggenenti,Roberta Donadelli,Ariela Benigni,Giuseppe RemuzziPubMed 35320941
- The British journal of dermatology 175:122-332016IgE and FcεRI are highly expressed on innate cells in psoriasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK-X Yan et. al.PubMed 26853903
- Scientific reports 6:212882016Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohanna Snäll et. al.PubMed 26887258
- mBio 6:e01390-152015Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah E Hochman et. al.PubMed 26396242
- The American journal of clinical nutrition 102:608-152015A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark D Farrar et. al.PubMed 26178731
- Thorax 51:64-701996Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Nasser,P E Christie,R Pfister,A R Sousa,A Walls,M Schmitz-Schumann,T H LeePubMed 8658372
- Histopathology 14:637-431989Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE Ralfkiaer,K A Pulford,A F Lauritzen,S Avnstrøm,B Guldhammer,D Y MasonPubMed 2759560
- Journal of clinical pathology 41:853-601988Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK A Pulford,W N Erber,J A Crick,I Olsson,K J Micklem,K C Gatter,D Y MasonPubMed 2844860
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Tested |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 11.16 µg/mL | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed:36899106). Promotes blood coagulation (PubMed:20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed:25211214, PubMed:9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed:20676107, PubMed:25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984).
Alternative names
ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE
Recommended products
Mouse Recombinant Monoclonal Neutrophil Elastase antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- NP57
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.
- Biological function summary
The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.
- Pathways
This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.
- Associated diseases and disorders
Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (ab254178), expandable thumbnail](https://content.abcam.com/products/images/neutrophil-elastase-antibody-np57-ab254178--immunocytochemistry-immunofluorescence-img22558.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (ab254178)")
Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] (ab254178)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labeling Neutrophil Elastase with ab254178 at 11.16μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on U-937 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab254178 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.Negative control: Jurkat（PMID: 22564522.
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