AB255935

Anti-Neutrophil Elastase antibody [NP57] - BSA and Azide free

Recombinant
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(8 Publications)

Mouse Recombinant Monoclonal Neutrophil Elastase antibody. Carrier free. Suitable for ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

ELA2, ELANE, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] - BSA and Azide free (AB255935)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] - BSA and Azide free (AB255935)

This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab254178).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labeling Neutrophil Elastase with ab254178 at 11.16μg/ml, followed by Goat Anti-Mouse IgG (Alexa Fluor^® 488) (ab150113) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on U-937 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab254178 at 1/50 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150113 AlexaFluor^®488 Goat anti-Mouse secondary at 1/1000 dilution.

Negative control : Jurkat（PMID : 22564522.

Unconjugated

Anti-Neutrophil Elastase antibody [NP57]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NP57

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "11.16 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

ab255935 is the carrier-free version of ab254178.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neutrophil Elastase also known as neutrophil proteinase 3 has a molecular mass of approximately 30 kDa. It is a serine protease expressed mainly in azurophilic granules of neutrophils. This enzyme breaks down various proteins including elastin at sites of inflammation. Neutrophil Elastase releases during degranulation or through neutrophil extracellular traps (NETs) to combat pathogens.

Biological function summary

The target plays a significant role in neutrophil-mediated tissue remodeling and host defense. It leads to the breaking down of extracellular matrix components contributing to tissue remodeling and repair. Neutrophil Elastase is part of a complex interplay with other proteases like matrix metalloproteinases (MMPs). Its activity is regulated by endogenous inhibitors such as alpha-1 antitrypsin to prevent excessive tissue damage.

Pathways

This protease is important in inflammation and immune response pathways. It plays a role in the complement system by activating C5 a component of the complement cascade. It also acts in conjunction with other proteases such as cathepsin G to modulate immune and inflammatory responses. Neutrophil Elastase's role within these pathways is pivotal for the regulation of inflammation and destruction of pathogens.

Individuals with conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis often exhibit dysregulation or excessive activity of Neutrophil Elastase. This excessive activity leads to tissue damage and exacerbates disease progression. The enzyme also interacts with proteins like elastin and collagen in these diseases further contributing to pathological tissue remodeling and damage.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed : 15140022). Promotes cleavage of GSDMB, thereby inhibiting pyroptosis (PubMed : 36899106). Promotes blood coagulation (PubMed : 20676107). Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG) (PubMed : 25211214, PubMed : 9111081). Cleaves and thus inactivates tissue factor pathway inhibitor (TFPI) (PubMed : 20676107, PubMed : 25211214). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed : 10947984).

See full target information ELANE

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:926788 PubMed36059509

2022

biofilm phenotypes confined but surviving in neutrophil extracellular traps of varying appearance.

Applications

Unspecified application

Species

Unspecified reactive species

Muthita Khamwong,Supranee Phanthanawiboon,Kanin Salao,Sorujsiri Chareonsudjai

The British journal of dermatology 175:122-33 PubMed26853903

2016

IgE and FcεRI are highly expressed on innate cells in psoriasis.

Applications

Unspecified application

Species

Unspecified reactive species

K-X Yan,Q Huang,X Fang,Z-H Zhang,L Han,K Gadaldi,K-F Kang,Z-Z Zheng,J-H Xu,N Yawalkar

Scientific reports 6:21288 PubMed26887258

2016

Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.

Applications

Unspecified application

Species

Unspecified reactive species

Johanna Snäll,Anna Linnér,Julia Uhlmann,Nikolai Siemens,Heike Ibold,Marton Janos,Adam Linder,Bernd Kreikemeyer,Heiko Herwald,Linda Johansson,Anna Norrby-Teglund

mBio 6:e01390-15 PubMed26396242

2015

Fatal Pediatric Cerebral Malaria Is Associated with Intravascular Monocytes and Platelets That Are Increased with HIV Coinfection.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Hochman,Theresa F Madaline,Samuel C Wassmer,Emmie Mbale,Namjong Choi,Karl B Seydel,Richard O Whitten,Julie Varughese,Georges E R Grau,Steve Kamiza,Malcolm E Molyneux,Terrie E Taylor,Sunhee Lee,Danny A Milner,Kami Kim

The American journal of clinical nutrition 102:608-15 PubMed26178731

2015

A randomized controlled trial of green tea catechins in protection against ultraviolet radiation-induced cutaneous inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Mark D Farrar,Anna Nicolaou,Kayleigh A Clarke,Sarah Mason,Karen A Massey,Tristan P Dew,Rachel E B Watson,Gary Williamson,Lesley E Rhodes

Thorax 51:64-70 PubMed8658372

1996

Effect of endobronchial aspirin challenge on inflammatory cells in bronchial biopsy samples from aspirin-sensitive asthmatic subjects.

Applications

Unspecified application

Species

Unspecified reactive species

S Nasser,P E Christie,R Pfister,A R Sousa,A Walls,M Schmitz-Schumann,T H Lee

Histopathology 14:637-43 PubMed2759560

1989

Diagnosis of acute myeloid leukaemia with the use of monoclonal anti-neutrophil elastase (NP57) reactive with routinely processed biopsy samples.

Applications

Unspecified application

Species

Unspecified reactive species

E Ralfkiaer,K A Pulford,A F Lauritzen,S Avnstrøm,B Guldhammer,D Y Mason

Journal of clinical pathology 41:853-60 PubMed2844860

1988

Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells.

Applications

Unspecified application

Species

Unspecified reactive species

K A Pulford,W N Erber,J A Crick,I Olsson,K J Micklem,K C Gatter,D Y Mason

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Neutrophil Elastase antibody [NP57] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Neutrophil Elastase antibody [NP57] - BSA and Azide free (AB255935)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise