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AB226322

Anti-Newcastle disease virus Hemagglutinin-neuraminidase antibody

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(1 Publication)

Rabbit Polyclonal HN antibody. Suitable for WB, IHC-P and reacts with Newcastle disease virus samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Newcastle disease virus (STRAIN B1-HITCHNER/47) HN aa 400-500 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Hemagglutinin-neuraminidase, HN

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Newcastle disease virus

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Newcastle disease virus (STRAIN B1-HITCHNER/47) HN aa 400-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P12559

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Newcastle disease virus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Newcastle disease virus Hemagglutinin-neuraminidase commonly referred to as HN is a multifunctional protein expressed on the surface of the Newcastle disease virus (NDV). This protein assists in viral attachment and spread by displaying both hemagglutination and neuraminidase activities. With a molecular mass of approximately 74 kDa HN binds sialic acid receptors on host cell surfaces to facilitate viral entry. It is expressed on the viral envelope and plays a role in promoting fusion with host cell membranes by interacting with the NDV fusion (F) protein.
Biological function summary

HN supports the infectivity of the Newcastle disease virus by binding to and cleaving sialic acid residues on glycoproteins across species. This cleavage releases newly formed viral particles from host cells enabling further spread within the host. It is an important element of the NDV as it forms transient complexes with other viral proteins such as the F protein. This interaction enhances the HN’s ability to mediate membrane fusion which is essential for the establishment of infection.

Pathways

The Newcastle disease virus Hemagglutinin-neuraminidase integrates into the viral replication pathway by influencing host-virus interactions and facilitating membrane fusion. It participates in the viral life cycle alongside pathways involving its close relative the F protein which contributes to the fusion of viral and host cell membranes. The interplay between HN and F protein in initiating and propagating infection highlights the protein's role in viral pathogenicity.

Newcastle disease virus Hemagglutinin-neuraminidase directly connects to Newcastle disease a highly contagious avian disease leading to significant economic impact in poultry farming. HN associates with the immune evasion strategies of NDV often leading to neurological and respiratory symptoms. In its viral context HN not only interacts with the F protein but also contributes to host immune system evasion perpetuating disease symptoms and complicating therapeutic intervention strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the viral entry into the host cell together with fusion/F protein. Attaches the virus to sialic acid-containing cell receptors and thereby initiates infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion.. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.
See full target information HN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal, genetic engineering & biotechnology 20:77 PubMed35608724

2022

Expression and characterization of hemagglutinin-neuraminidase protein from Newcastle disease virus in Bacillus subtilis WB800.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammadreza Shafaati,Masoud Ghorbani,Minoo Mahmoodi,Mostafa Ebadi,Reza Jalalirad
View all publications
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