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AB233235

Anti-Nexilin/F-actin-binding protein antibody

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(2 Publications)

Rabbit Polyclonal Nexilin/F-actin-binding protein antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Nexn aa 200-500.

View Alternative Names

Nexilin, Nexn

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233235)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233235)

Formalin-fixed, paraffin-embedded rat cardiac muscle tissue stained for Nexilin/F-actin-binding protein with ab233235 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)
  • WB

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/mL

All lanes:

Rat testis tissue lysate

Predicted band size: 81 kDa

false

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)
  • WB

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/mL

All lanes:

Rat cerebrum tissue lysate

Predicted band size: 81 kDa

false

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)
  • WB

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/mL

All lanes:

Rat lung tissue lysate

Predicted band size: 81 kDa

false

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)
  • WB

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/mL

All lanes:

Rat heart tissue lysate

Predicted band size: 81 kDa

false

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)
  • WB

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233235)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233235) at 1 µg/mL

All lanes:

Recombinant rat Nexilin/F-actin-binding protein

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Rat Nexn aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

Q9Z2J4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.20000-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nexilin also known as F-actin-binding protein serves vital role in the mechanical stability of cells. It binds to actin filaments reinforcing structural integrity important for muscle function. Nexilin has a molecular mass around 75 kDa. This protein sees expression mainly in cardiac and skeletal muscle tissues. Actin-binding proteins like nexilin stabilize the actin cytoskeleton which is important for maintaining structure of muscle cells during contraction and relaxation.
Biological function summary

Nexilin contributes significantly to maintaining the integrity of muscle cells. It stabilizes actin filaments in sarcomeres which are the basic units of muscle contraction. Nexilin forms part of a complex that interacts with other structural proteins like actin-binding proteins which together help preserve cellular architecture under mechanical stress. This function is essential for normal heart muscle function and response to mechanical load.

Pathways

Nexilin plays a role in cardiac muscle contraction and signal transduction pathways. The protein integrates into the myofibril assembly process ensuring efficient communication within muscle cells. Pathways involving nexilin link with proteins such as titin assisting with elasticity and tension. These pathways maintain cardiac function under mechanical strain reflecting nexilin's actin-binding protein role in muscle physiology.

Mutations or dysfunctions in nexilin connect to dilated cardiomyopathy and other cardiac diseases. Abnormal nexilin expression affects structural stability leading to heart muscle weakness. The link with proteins like desmin a cytoskeletal protein associates nexilin to these conditions. Understanding nexilin’s interactions could offer insights into heart disease treatment emphasizing its importance in cardiovascular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.
See full target information Nexn
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4433 PubMed35907876

2022

CDC-like kinase 4 deficiency contributes to pathological cardiac hypertrophy by modulating NEXN phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Huang,Luxin Wang,Yunli Shen,Shengqi Zhang,Yaqun Zhou,Jimin Du,Xiue Ma,Yi Liu,Dandan Liang,Dan Shi,Honghui Ma,Li Li,Qi Zhang,Yi-Han Chen

The Journal of clinical investigation 129:1115-1128 PubMed30589415

2018

Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Wei Hu,Feng-Xia Guo,Yuan-Jun Xu,Pan Li,Zhi-Feng Lu,David G McVey,Lei Zheng,Qian Wang,John H Ye,Chun-Min Kang,Shao-Guo Wu,Jing-Jing Zhao,Xin Ma,Zhen Yang,Fu-Chun Fang,Yu-Rong Qiu,Bang-Ming Xu,Lei Xiao,Qian Wu,Li-Mei Wu,Li Ding,Tom R Webb,Nilesh J Samani,Shu Ye
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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