AB233267

Anti-Nexilin/F-actin-binding protein antibody

Name: Anti-Nexilin/F-actin-binding protein antibody (ab233267) | Abcam
Brand: Abcam Limited
SKU: AB233267
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(2 Publications)

Rabbit Polyclonal Nexilin/F-actin-binding protein antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nexn aa 400 to C-terminus.

View Alternative Names

Nexilin, F-actin-binding protein, Nexn

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Formalin-fixed, paraffin-embedded mouse testis tissue stained for Nexilin/F-actin-binding protein with ab233267 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Formalin-fixed, paraffin-embedded mouse heart tissue stained for Nexilin/F-actin-binding protein with ab233267 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Nexilin/F-actin-binding protein with ab233267 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Nexilin/F-actin-binding protein with ab233267 at 10 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 2 μg/ml. DAB staining.

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233267) at 3 µg/mL

All lanes:

Recombinant mouse Nexilin/F-actin-binding protein protein

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

All lanes:

Western blot - Anti-Nexilin/F-actin-binding protein antibody (ab233267) at 3 µg/mL

Lane 1:

Mouse heart tissue lysate

Lane 2:

Mouse lung tissue lysate

Lane 3:

Mouse liver tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/1000 dilution

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Nexn aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q7TPW1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nexilin also known as F-actin-binding protein serves vital role in the mechanical stability of cells. It binds to actin filaments reinforcing structural integrity important for muscle function. Nexilin has a molecular mass around 75 kDa. This protein sees expression mainly in cardiac and skeletal muscle tissues. Actin-binding proteins like nexilin stabilize the actin cytoskeleton which is important for maintaining structure of muscle cells during contraction and relaxation.

Biological function summary

Nexilin contributes significantly to maintaining the integrity of muscle cells. It stabilizes actin filaments in sarcomeres which are the basic units of muscle contraction. Nexilin forms part of a complex that interacts with other structural proteins like actin-binding proteins which together help preserve cellular architecture under mechanical stress. This function is essential for normal heart muscle function and response to mechanical load.

Pathways

Nexilin plays a role in cardiac muscle contraction and signal transduction pathways. The protein integrates into the myofibril assembly process ensuring efficient communication within muscle cells. Pathways involving nexilin link with proteins such as titin assisting with elasticity and tension. These pathways maintain cardiac function under mechanical strain reflecting nexilin's actin-binding protein role in muscle physiology.

Mutations or dysfunctions in nexilin connect to dilated cardiomyopathy and other cardiac diseases. Abnormal nexilin expression affects structural stability leading to heart muscle weakness. The link with proteins like desmin a cytoskeletal protein associates nexilin to these conditions. Understanding nexilin’s interactions could offer insights into heart disease treatment emphasizing its importance in cardiovascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.

See full target information Nexn

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Drug design, development and therapy 17:1819-1829 PubMed37360572

2023

Luteolin Alleviates Liver Fibrosis in Rat Hepatic Stellate Cell HSC-T6: A Proteomic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Batudeligen,Zhiqiang Han,Hongmei Chen, Narisu,Yanhua Xu, Anda,Gegentaoli Han

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:3344-3364 PubMed35273083

2022

Formation of the Mouse Internal Capsule and Cerebral Peduncle: A Pioneering Role for Striatonigral Axons as Revealed in Conditional Mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Jacqueline M Ehrman,Paloma Merchan-Sala,Lisa A Ehrman,Bin Chen,Hee-Woong Lim,Ronald R Waclaw,Kenneth Campbell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nexilin/F-actin-binding protein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Western blot - Anti-Nexilin/F-actin-binding protein antibody (AB233267)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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