Rabbit Polyclonal NF-kB p65 acetyl K310 antibody. Suitable for IP, Dot, WB and reacts with Mouse, Rat, Synthetic peptide samples. Cited in 124 publications.
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- Acta neuropathologica communications 11:1822023Neuronal dysfunction caused by FUSR521G promotes ALS-associated phenotypes that are attenuated by NF-κB inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMari Carmen Pelaez,Antoine Desmeules,Pauline A Gelon,Bastien Glasson,Laetitia Marcadet,Alicia Rodgers,Daniel Phaneuf,Silvia Pozzi,Paul A Dutchak,Jean-Pierre Julien,Chantelle F SephtonPubMed 37974279
- The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 27:533-5402023Sweroside plays a role in mitigating high glucose-induced damage in human renal tubular epithelial HK-2 cells by regulating the SIRT1/NF-κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodan Ma,Zhixin Guo,Wenhua Zhao,Li ChenPubMed 37884285
- Science advances 9:eadi83432023Deficient chaperone-mediated autophagy facilitates LPS-induced microglial activation via regulation of the p300/NF-κB/NLRP3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Wu,Yingying Han,Hao Xu,Hongyang Sun,Rui Wang,Haigang Ren,Guanghui WangPubMed 37801503
- iScience 26:1081302023LRRK2 G2019S promotes astrocytic inflammation induced by oligomeric α-synuclein through NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Jie He,Jin-Bao Zhang,Jun-Yi Liu,Feng-Lun Zhao,Xiao-Yu Yao,Yu-Ting Tang,Jin-Ru Zhang,Xiao-Yu Cheng,Li-Fang Hu,Fen Wang,Chun-Feng LiuPubMed 37876795
- Nutrients 15:2023Nicotinamide Prevents Diabetic Brain Inflammation via NAD+-Dependent Deacetylation Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeimy Katherine Torres-Méndez,Julia Niño-Narvión,Patricia Martinez-Santos,Elena María Goretti Diarte-Añazco,Karen Alejandra Méndez-Lara,Tania Vázquez Del Olmo,Noemi Rotllan,Maria Teresa Julián,Núria Alonso,Didac Mauricio,Mercedes Camacho,Juan Pablo Muñoz,Joana Rossell,Josep JulvePubMed 37513501
- Life science alliance 6:2023B-cell targeting with anti-CD38 daratumumab: implications for differentiation and memory responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDorit Verhoeven,Lucas Grinwis,Casper Marsman,Machiel H Jansen,Ester Mm Van Leeuwen,Taco W KuijpersPubMed 37419630
- Frontiers in pharmacology 14:11739202023Fresh Blume alleviates simulated weightlessness-induced cognitive impairment by regulating inflammatory and apoptosis-related pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiwen Zhang,Hong Huang,Caihong Yao,Xinran Sun,Qinghu He,Muhammad Iqbal Choudharyc,Shanguang Chen,Xinmin Liu,Ning JiangPubMed 37205911
- iScience 26:1066442023SIRT2 inhibition by AGK2 enhances mycobacteria-specific stem cell memory responses by modulating beta-catenin and glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAshima Bhaskar,Isha Pahuja,Kriti Negi,Akanksha Verma,Antara Ghoshal,Babu Mathew,Gaurav Tripathi,Jaswinder Singh Maras,Shivam Chaturvedi,Ved Prakash DwivediPubMed 37192966
- Frontiers in pharmacology 14:11269722023Effect of botanical drugs in improving symptoms of hypertensive nephropathy: Analysis of real-world data, retrospective cohort, network, and experimental assessment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Ming Huan,Xi-Ting Ma,Si-Yi Li,Dong-Qing Hu,Hao-Yu Chen,Yi-Min Wang,Xiao-Yi Su,Wen-Ge Su,Yi-Fei WangPubMed 37089916
- European journal of histochemistry : EJH 67:2023Effects of piperlonguminine on lung injury in severe acute pancreatitis <em>via</em> the TLR4/NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Hu,Ran Tao,Xiaoyun Hu,Haibo Wu,Jianjun XuPubMed 36951266
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IP | Dot | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Predicted | Predicted | Predicted | Not recommended |
| Mouse | Tested | Expected | Tested | Not recommended |
| Rat | Expected | Expected | Tested | Not recommended |
| Synthetic peptide | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Collaborator data suggests that immunoprecipitation of this antibody prior to Western blotting is required to obtain the best results (see images) |
Species Rat | Dilution info 2.5 µg/mL | Notes Collaborator data suggests that immunoprecipitation of this antibody prior to Western blotting is required to obtain the best results (see images) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
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Target data
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Recommended products
Rabbit Polyclonal NF-kB p65 acetyl K310 antibody. Suitable for IP, Dot, WB and reacts with Mouse, Rat, Synthetic peptide samples. Cited in 124 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.
- Biological function summary
NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.
- Pathways
NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.
- Associated diseases and disorders
NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
This image is courtesy of Christine Buerki and Karin Rothgiesser, University of Zurich, SwitzerlandImmunoprecipitation - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)
Western Blot with ab19870 after p65 Immunoprecipitation: rabbit polyclonal to NF-kB p65 (acetyl K310) (ab19870; 2.5μg/ml) in 1% non-fat milk TBS-T incubated for 3 hours at room temperature. Exposure time: 1 min normal ECL. Tested samples: nuclear extracts (180 μg) of immortalized p65-/- mouse cells, complemented with the empty vector (pRRL), wild-type p65 (Wt) and non-acetylatable K310 (K310R). The samples tested were treated with deacetylase inhibitors HDACi (TSA + Nicotinamide) and TNF-alpha. The samples were immunoprecipitated with 2μg of alpha-p65 and subsequently analysed by Western blot with Rabbit polyclonal to NF-kB p65 (acetyl K310) (ab19870). Predicted band size = 65kDa, Observed band size = 75kDa. The p65 band runs higher in this SDS-PAGE blot as it contains a myc-tag.
All lanes: Immunoprecipitation - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)
Predicted band size: 60 kDa
This image is courtesy of Prof. Michael Hottiger's Lab, University of ZurichWestern blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)
Observed band size: 75kDaThe p65 band runs higher in this blot because the protein contains a myc-tag.
Lanes 1 - 2: Coomassie stain
Lanes 3 - 4: Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870) at 1/500 dilution
Lanes 1 and 3: Acetylated p65 protein at 2 µg
Lanes 2 and 4: K310R mutant protein at 2 µg
SecondaryAll lanes: IRDye® 800CW Goat anti-Rabbit IgG (H + L) at 1/15000 dilution
Predicted band size: 60 kDa
This image is courtesy of Christine Buerki and Karin Rothgiesser, University of Zurich, SwitzerlandDot Blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)
Rabbit polyclonal to NF-kB p65 (acetyl K310) (ab19870; 2.5μg/ml) in 1% non-fat milk TBS-T incubated for 3h at room temperature. Exposure time: 75 min normal ECL. This Dot blot demonstrates that ab19870 recognized upto 10ng of purified peptide on a PVDF membrane.
This image is courtesy of Christine Buerki and Karin Rothgiesser, University of Zurich, SwitzerlandWestern blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)
ab19870 recognizes Rabbit polyclonal to NF-kB p65 (acetyl K310) specifically at ~75kDa (indicated by the arrow) is this SDS-PAGE blot. The p65 band runs higher than 65kDa in this SDS-PAGE blot as it contains a myc-tag. We are sure that the band at ~75kDa is p65 since p65 specific antibodies detect the same band in IP and WB and there is no signal in the p65 knock-out cell line with ab19870. A number of additonal bands are recognized by ab19870 when tested with endogenous p65 from whole cell extracts, we do not know the identity of these bands.
Tested samples: nuclear extracts (75μg) of immortalized p65-/- mouse cells, complemented with the empty ve
All lanes: Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870) at 2.5 µg/mL
Lane 1: pRRL untreated at 75 µg
Lane 2: pRRL HDACi at 75 µg
Lane 3: pRRL HDACi + TNF at 75 µg
Lane 4: Wt untreated at 75 µg
Lane 5: Wt HDACi at 75 µg
Lane 6: Wt HDACi + phorbol myristate acetate at 75 µg
Lane 7: K310R untreated at 75 µg
Lane 8: K310R HDACi at 75 µg
Lane 9: K310R HDACi + phorbol myristate acetate at 75 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 75 kDa
Exposure time: 1hr
Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870)
All lanes: Western blot - Anti-NF-kB p65 (acetyl K310) antibody (ab19870) at 1 µg/mL
All lanes: Lung (Rat) Tissue Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 15 kDa, 72 kDa
Exposure time: 4min
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