Rabbit Recombinant Monoclonal NF-kB p65 acetyl K310 antibody. Carrier free. Suitable for ChIP, Dot, WB and reacts with Human, Synthetic peptide, Recombinant fragment - Human samples. Cited in 3 publications.
Images
![ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (AB237591), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-acetyl-k310-antibody-epr21781-bsa-and-azide-free-ab237591--chip-img14038.jpg/_jcr_content/renditions/webp-475.webp "ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (AB237591)")
![Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (AB237591), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-acetyl-k310-antibody-epr21781-bsa-and-azide-free-ab237591--western-blot-img40676.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (AB237591)")
![Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (AB237591), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-acetyl-k310-antibody-epr21781-bsa-and-azide-free-ab237591--dot-blot-img7534.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (AB237591)")
Publications
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- Frontiers in behavioral neuroscience 17:11720912023Resveratrol ameliorates maternal separation-induced anxiety- and depression-like behaviors and reduces Sirt1-NF-kB signaling-mediated neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu-Meng Wei,Yue-Ming Zhang,Yi-Zhou Feng,Kai-Xuan Zhang,Jing-Ya Zhang,Jing Chen,Bao-Ling Luo,Xue-Yan Li,Gui-Hai ChenPubMed 37273278
- Cancer management and research 12:497-5092020Procyanidin Compound (PC) Suppresses Lipopolysaccharide-Induced Cervical Cancer Cell Proliferation Through Blocking the TLR4/NF-κB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan Yang et. al.PubMed 32158256
- Nano letters 19:4879-48892019Bioresponsive Protein Complex of aPD1 and aCD47 Antibodies for Enhanced Immunotherapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Chen et. al.PubMed 31294571
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | Dot | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested |
| Synthetic peptide | Not recommended | Tested | Not recommended |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide, Recombinant fragment - Human | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Recommended products
Rabbit Recombinant Monoclonal NF-kB p65 acetyl K310 antibody. Carrier free. Suitable for ChIP, Dot, WB and reacts with Human, Synthetic peptide, Recombinant fragment - Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21781
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab237591 is the carrier-free version of Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.
- Biological function summary
NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.
- Pathways
NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.
- Associated diseases and disorders
NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-acetyl-k310-antibody-epr21781-bsa-and-azide-free-ab237591--chip-img14038.jpg "ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)")
ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)
Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with and without 20 ng/ml TNF-α for 60 minutes according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).
The ChIP data are consistent with the literature (PMID: 16135789).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533).
![Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-acetyl-k310-antibody-epr21781-bsa-and-azide-free-ab237591--western-blot-img40676.jpg "Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)")
Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)
Blocking/Dilution buffer: 5% NFDM/TBST.
NF-κB p65 (acetyl K310) expression is induced by TNF-α and p300 acetyltransferases (PMID: 20160011, PMID: 12456660, PMID: 16135789).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533).
All lanes: Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591) at 1/2000 dilution
Lane 1: HEK-293 transfected with NF-kB p65 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2: HEK-293 transfected with NF-kB p65 and p300 (aa1287-1663) expression vectors containing a myc-His-tag®, then treated with 20 ng/ml TNF-alpha for 60 minutes, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 60 kDa
Observed band size: 70 kDa
![Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-acetyl-k310-antibody-epr21781-bsa-and-azide-free-ab237591--dot-blot-img7534.jpg "Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)")
Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - BSA and Azide free (ab237591)
Dot blot analysis of NF-kB p65 (acetyl K310) labeled with Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533 at 1/1000 dilution.
Lane 1: NF-κB p65 (acetyl K310) peptide.
Lane 2: NF-κB p65 non-acetyl peptide.Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 2 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade ab218533).
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