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Rabbit Recombinant Monoclonal NF-kB p65 acetyl K310 antibody. Suitable for ChIP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell lysate - Human samples. Cited in 1 publication.

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Images

ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533), expandable thumbnail
  • Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533), expandable thumbnail
  • Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533), expandable thumbnail
  • Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533), expandable thumbnail
  • Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (AB218533), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPDotWB
Human
Tested
Expected
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Transfected cell lysate - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
2 µg for 25 µg chromatin
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Alternative names

NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Recommended products

Rabbit Recombinant Monoclonal NF-kB p65 acetyl K310 antibody. Suitable for ChIP, Dot, WB and reacts with Human, Synthetic peptide, Transfected cell lysate - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21781
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.

Biological function summary

NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.

Pathways

NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.

Associated diseases and disorders

NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533), expandable thumbnail

    ChIP - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533)

    Chromatin was prepared from HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with and without 20 ng/ml TNF-α (Recombinant human TNF alpha protein ab9642) for 60 minutes according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 5 μg of ab218533 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach).

    The ChIP data are consistent with the literature (PMID: 16135789).

  • Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533), expandable thumbnail

    Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    We used a high sensitivity ECL substrate to develop this blot.

    All lanes: Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/1000 dilution

    All lanes: Human lung tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 60 kDa

    Observed band size: 65 kDa

    Exposure time: 3min

  • Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533), expandable thumbnail
    This image is courtesy of an anonymous collaborator.

    Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533)

    The p65 band runs higher in this blot because the protein contains a myc-tag.

    Lanes 1 - 2: Coomassie stain

    Lanes 3 - 4: Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/1000 dilution

    Lanes 1 and 3: Acetylated p65 protein at 2 µg

    Lanes 2 and 4: K310R mutant protein at 2 µg

    Secondary

    All lanes: IRDye® 800CW Goat anti-Rabbit IgG (H + L) at 1/15000 dilution

    Predicted band size: 60 kDa

    Observed band size: 75 kDa

  • Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533), expandable thumbnail

    Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    NF-κB p65 (acetyl K310) expression is induced by TNF-α and p300 acetyltransferases (PMID: 20160011, PMID: 12456660, PMID: 16135789).

    All lanes: Western blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533) at 1/2000 dilution

    Lane 1: HEK-293 transfected with NF-kB p65 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

    Lane 2: HEK-293 transfected with NF-kB p65 and p300 (aa1287-1663) expression vectors containing a myc-His-tag®, then treated with 20 ng/ml TNF-alpha (Recombinant human TNF alpha protein ab9642) for 60 minutes, whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa

    Observed band size: 70 kDa

    Exposure time: 37s

  • Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533), expandable thumbnail

    Dot Blot - Anti-NF-kB p65 (acetyl K310) antibody [EPR21781] - ChIP Grade (ab218533)

    Dot blot analysis of NF-kB p65 (acetyl K310) labeled with ab218533 at 1/1000 dilution.

    Lane 1: NF-κB p65 (acetyl K310) peptide.
    Lane 2: NF-κB p65 non-acetyl peptide.

    Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 2 minutes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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