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Rabbit Recombinant Monoclonal NF-kB p65 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Mouse, Human samples. Cited in 72 publications.


Images

Immunoprecipitation - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (AB207297), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (AB207297), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (AB207297), expandable thumbnail
  • Western blot - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (AB207297), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (AB207297), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

5 products for Alternative Version

Target data

Function

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).

Alternative names

Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, NFKB3, RELA

Recommended products

Rabbit Recombinant Monoclonal NF-kB p65 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Mouse, Human samples. Cited in 72 publications.

Alternative names

Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, NFKB3, RELA

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

E379

Purification technique

Affinity purification Protein A

Specificity

This antibody recognises NF-kB p65. For WB, this antibody is unsuitable for detecting NF-KB p65 in mouse tissue lysates. The expression of NF-KB p65 is increased by lipopolysaccharides treatment reported by PMID: 18036230. Although some papers support the expression of NF-κB p65 in mouse tissue (PMID: 21479220 and 20008488), This antibody cannot detect band of interest in these mouse tissue.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab207297 is the carrier-free version of Anti-NF-kB p65 antibody [E379] ab32536.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.

Biological function summary

NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.

Pathways

NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.

Associated diseases and disorders

NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunoprecipitation - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunoprecipitation - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Anti-NF-kB p65 antibody [E379] ab32536 (purified) at 1:30 dilution (2μg) immunoprecipitating NF-kB p65 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2 (+): Anti-NF-kB p65 antibody [E379] ab32536 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NF-kB p65 antibody [E379] ab32536 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [E379] ab32536).

    All lanes: Immunoprecipitation - Anti-NF-kB p65 antibody [E379] (Anti-NF-kB p65 antibody [E379] ab32536)

    Predicted band size: 60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Clone E379 (ab207297) has been successfully conjugated by Abcam. This image was generated using Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 488). Please refer to Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] ab190205 for protocol details.

    Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] ab190205 staining NF-kB p65 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 488 Anti-NF-kB p65 antibody [E379] ab190205 at a working dilution of 1 in 50 (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at a dilution of 1 in 250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal in 4% formaldehyde (10 min) fixed HeLa cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Clone E379 (ab207297) has been successfully conjugated by Abcam. This image was generated using Anti-NF-kB p65 antibody [E379] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-NF-kB p65 antibody [E379] ab190589 for protocol details.

    Alexa Fluor® 647 Anti-NF-kB p65 antibody [E379] ab190589 staining NF-kB p65 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with Alexa Fluor® 647 Anti-NF-kB p65 antibody [E379] ab190589 at a working dilution of 1 in 100 (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Western blot - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Western blot - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    This WB data was generated using the same anti-NF-kB p65 antibody clone, E379, in a different buffer formulation (cat# Anti-NF-kB p65 antibody [E379] ab32536).

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: NF-kB p65 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: A431 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-NF-kB p65 antibody [E379] ab32536 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control ab8245 loading control, observed at 37 kDa.


    Anti-NF-kB p65 antibody [E379] ab32536 was shown to specifically react with NF-kB p65 in wild-type HAP1 cells. No band was observed when NF-kB p65 knockout samples were used. Wild-type and NF-kB p65 knockout samples were subjected to SDS-PAGE. Anti-NF-kB p65 antibody [E379] ab32536 (NF-kB p65) and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted to 1/50 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-NF-kB p65 antibody [E379] (Anti-NF-kB p65 antibody [E379] ab32536)

    Predicted band size: 60 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NF-kB p65 with purified Anti-NF-kB p65 antibody [E379] ab32536 at 1:100 dilution. Cells were fixed in 100% Methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [E379] ab32536).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    This data was generated using the same anti-NF-kB p65 antibody clone, E379, in a different buffer formulation (Anti-NF-kB p65 antibody [E379] ab32536).

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NF-kB p65 with Anti-NF-kB p65 antibody [E379] ab32536 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse spleen. The section was incubated with Anti-NF-kB p65 antibody [E379] ab32536 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    This data was generated using the same anti-NF-kB p65 antibody clone, E379, in a different buffer formulation (Anti-NF-kB p65 antibody [E379] ab32536).

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling NF-kB p65 with Anti-NF-kB p65 antibody [E379] ab32536 at 1/100 (4.89 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing the signal translocated from the cytoplasm into the nucleus in NIH/3T3 cells after the treatment with TNF-alpha (50 ng/ml) for 20 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution in treated (right) and untreated cells (left).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    This data was generated using the same anti-NF-kB p65 antibody clone, E379, in a different buffer formulation (Anti-NF-kB p65 antibody [E379] ab32536).

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NF-kB p65 with Anti-NF-kB p65 antibody [E379] ab32536 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human tonsil. The section was incubated with Anti-NF-kB p65 antibody [E379] ab32536 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    This IHC data was generated using the same anti-NF-kB p65 antibody clone, E379, in a different buffer formulation (cat# Anti-NF-kB p65 antibody [E379] ab32536).

    Immunohistochemical analysis of colon sections from mice, staining NF-kB p65 with Anti-NF-kB p65 antibody [E379] ab32536.

    Antigen retrieval was performed by microwave heating in citrate buffer, pH 6. Sections were incubated overnight with primary antibody (1/250) and staining was detected using ab80437 EXPOSE Rabbit specific HRP/DAB detection IHC kit.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    This data was generated using the same anti-NF-kB p65 antibody clone, E379, in a different buffer formulation (Anti-NF-kB p65 antibody [E379] ab32536).

    Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling NF-kB p65 with Anti-NF-kB p65 antibody [E379] ab32536 at 1/5000 (0.098 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human prostatic hyperplasia. The section was incubated with Anti-NF-kB p65 antibody [E379] ab32536 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling NF-kB p65 with Purified Anti-NF-kB p65 antibody [E379] ab32536 at 1:2000 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [E379] ab32536).

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Clone E379 (ab207297) has been successfully conjugated by Abcam. This image was generated using Anti-NF-kB p65 antibody [E379] (PE). Please refer to PE Anti-NF-kB p65 antibody [E379] ab208750 for protocol details.

    PE Anti-NF-kB p65 antibody [E379] ab208750 staining NF-kB p65 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with PE Anti-NF-kB p65 antibody [E379] ab208750 at 1/500 dilution (Pseudocolored in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail
    Image from Ali Ahmed Atef Ahmed et al. PLoS ONE 11.4 (2016): e0154278. Fig 8. doi: 10.1371/journal.pone.0154278.

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Immunocytochemistry/ Immunofluorescence analysis of human cancer cells labeling NF-kB p65 with unpurified Anti-NF-kB p65 antibody [E379] ab32536. Briefly, the tested cells were seeded on coverslips treated with HCl and ethanol, and autoclaved prior to use. Immunostaining of the p65 subunit of NF-κB was done by permeabilizing the cells with Triton X-10, then by treating the cells with anti-NF-κB p65 rabbit monoclonal primary antibody [E379] (Anti-NF-kB p65 antibody [E379] ab32536), followed by Alexa Fluor® 488 Donkey anti-rabbit IgG secondary antibody. Nuclei of cells were stained with DAPI. Images were acquired using fluorescence microscope. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [E379] ab32536).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Immunohistochemical analysis of paraffin-embedded human Breast carcinoma using unpurified anti-NF-kB p65 Rabbit Monoclonal Antibody

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [E379] ab32536).

  • Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [E379] - BSA and Azide free (ab207297)

    Immunofluorescent staining of HeLa cells using anti-NF-kB p65 Rabbit Monoclonal Antibody ( unpurified Anti-NF-kB p65 antibody [E379] ab32536)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [E379] ab32536).

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