Rabbit Recombinant Monoclonal NF-kB p65 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--western-blot-img58974.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347)")
![Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--immunocytochemistry-immunofluorescence-img110538.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347)")
![Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--flow-cytometry-intracellular-img177367.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347)")
![Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--flow-cytometry-intracellular-img176869.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (AB246347)")
Publications
Filter by
- Molecular medicine reports 20:3085-30942019Rapamycin improves sevoflurane‑induced cognitive dysfunction in aged rats by mediating autophagy through the TLR4/MyD88/NF‑κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Li et. al.PubMed 31432123
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
2 products for Alternative Version
Target data
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Additional Targets
RELA
Alternative names
NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Recommended products
Rabbit Recombinant Monoclonal NF-kB p65 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2161Y
- Purification technique
- Affinity purification Protein A
- Specificity
ab246347 detects both phosphorylated and non-phosphorylated versions of human NF-kB p65.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab246347 is the carrier-free version of Anti-NF-kB p65 antibody [EP2161Y] ab76311.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.
- Biological function summary
NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.
- Pathways
NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.
- Associated diseases and disorders
NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--western-blot-img58974.jpg "Western blot - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)")
Western blot - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: NF-κB p65 knockout HAP1 cell lysate (20 μg)
Lane 3: HeLa cell lysate (20 μg)
Lane 4: A431 cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-NF-kB p65 antibody [EP2161Y] ab76311 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control ab8245 loading control, observed at 37 kDa.
Anti-NF-kB p65 antibody [EP2161Y] ab76311 was shown to specifically react with NF-κB p65 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when NF-κB p65 knockout samples were used. Wild-type and NF-κB p65 knockout samples were subjected to SDS-PAGE. Anti-NF-kB p65 antibody [EP2161Y] ab76311 (NF-κB p65) and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [EP2161Y] ab76311).
All lanes: Western blot - Anti-NF-kB p65 antibody [EP2161Y] (Anti-NF-kB p65 antibody [EP2161Y] ab76311)
Predicted band size: 60 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--immunocytochemistry-immunofluorescence-img110538.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)")
Immunocytochemistry/ Immunofluorescence - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)
Immunocytochemistry/Immunofluorescence analysis HT-29 (human colorectal adenocarcinoma) labelling NF-kB p65 with purified Anti-NF-kB p65 antibody [EP2161Y] ab76311 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 antibody [EP2161Y] ab76311).
![Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--flow-cytometry-intracellular-img177367.jpg "Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)")
Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)
This data was developed using Anti-NF-kB p65 antibody [EP2161Y] ab76311, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labeling NF-kB p65 with purified Anti-NF-kB p65 antibody [EP2161Y] ab76311 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-antibody-ep2161y-bsa-and-azide-free-ab246347--flow-cytometry-intracellular-img176869.jpg "Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)")
Flow Cytometry (Intracellular) - Anti-NF-kB p65 antibody [EP2161Y] - BSA and Azide free (ab246347)
This data was developed using Anti-NF-kB p65 antibody [EP2161Y] ab76311, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling NF-kB p65 with purified Anti-NF-kB p65 antibody [EP2161Y] ab76311 at 1/1000 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com