Anti-NF-kB p65 (phospho S536) antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF-kB p65 (phospho S536) antibody (AB86299)

IHC image of NF-kB p65 (phospho S536) staining in Human ovarian carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86299, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

• WB

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Western blot - Anti-NF-kB p65 (phospho S536) antibody (AB86299)

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-NF-kB p65 (phospho S536) antibody (ab86299) at 0.1 µg/mL

Lane 1:

Jurkat whole cell lysate treated with TNF alpha and Calyculin A at 50 µg

Lane 2:

Jurkat whole cell lysate treated with TNF alpha and Calyculin A at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 60 kDa

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• WB

CiteAb

Western blot - Anti-NF-kB p65 (phospho S536) antibody (AB86299)

NF-kB p65 western blotting using Anti-NF-kB p65 (phospho S536) antibody ab86299. Publication image and figure legend from Chen, L., Xie, Z. Y., et al., 2019, Cell Prolif, Pubmed 30430692.

Role of nuclear factor-kappa B (NF-κB) signalling in rat NPC proliferation induced by tumour necrosis factor alpha (TNF-α). The cells were exposed to 10 ng/mL TNF-α with or without p65 siRNA (siP65) pretreatment for 24 h. A,B, NF-κB-p65 interference was verified through Western blot analysis. Proteins were extracted to detect the expression of p-p65 and p65. The expression levels of p-p65 were normalized to that of p65. C, Cell proliferation was evaluated through CCK-8 analysis at different time points. D,E, Immunofluorescence staining of Ki67 (original magnification, x200) and quantification of Ki67-positive cells. F,G, The proportions of cells in each phase were examined through flow cytometry. H,I, Cyclin D1 and cyclin B1 expression levels in NPCs subjected to different treatments were measured through Western blot analysis and normalized to that of vinculin. The results were presented as mean ± SD (n = 3). *p < < 0.05; **p < < 0.01

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• WB

CiteAb

Western blot - Anti-NF-kB p65 (phospho S536) antibody (AB86299)

NF-kB p65 western blotting using Anti-NF-kB p65 (phospho S536) antibody ab86299. Publication image and figure legend from Wu, S. J., Lin, Z. H., et al., 2020, Front Physiol, Pubmed 32116751.

(A-E) western blotting demonstrated protein expression in the AMPK pathway, Cx43, fibrosis, pro-inflammatory cytokines, and TLR-4-NF-κB pathway. Dex increased the phosphorylation of AMPK and the expression of Cx43 in the ICM + Dex group. Dex also reduced the expression of collagens, TGF-β, and pro-inflammatory cytokines. There was less activation of the TLR-4-NF-κB pathway in the ICM + Dex group than in the ICM group. BML blunted the effect of Dex on the AMPK pathway, Cx43, fibrosis, pro-inflammatory cytokines, and the TLR-4-NF-κB pathway. Data are expressed as mean ± SD. *p < < 0.05 vs. Sham group, #p < < 0.05 vs. ICM group, δp < < 0.05 vs. ICM + Dex group (n = 6 for each group).

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• WB

CiteAb

Western blot - Anti-NF-kB p65 (phospho S536) antibody (AB86299)

NF-kB p65 western blotting using Anti-NF-kB p65 (phospho S536) antibody ab86299. Publication image and figure legend from Zhang, S., Hu, L., et al., 2020, J Neuroinflammation, Pubmed 31924219.

Ds-HMGB1 induced increased NF-κB activation resulted from disruption of microglial autophagy. a Representative immunoblot bands of LC3 and SQSTM1/p62 in different groups of microglia. b, c Optical density analysis of LC3 and SQSTM1/p62 immunoblot bands. d the mRNA expression of IL-1β and TNF-α was increased in ds-HMGB1-treated microglia in vitro. e NF-κB p65 was detected from nuclear and cytosolic fractions extracted from microglia using western immunoblot. f-h Optical density analysis of phosphorylation of NF-κB p65 bands both in nuclear and cytosolic fractions of microglia. β-actin and histone were used as the cytoplasmic or nuclear loading control, respectively. Data are presented as mean ± SEM. n = 6, *p < < 0.05, ANOVA LSD test

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