Anti-NF-kB p65 (phospho S536) antibody EP2294Y is a rabbit monoclonal antibody that is used in NF-kB p65 (phospho S536) Western blot, IHC, ICC/IF, Flow cytometry, IP. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 240 publications
- Trusted since 2009
Images
![Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--immunoprecipitation-img137564.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)")
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--western-blot-img11278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)")
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--western-blot-img11548.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)")
![Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--dot-blot-img4543.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (AB76302)")
Publications
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- International journal of molecular sciences 24:2023Protective Effect and Mechanism of Aspirin Eugenol Ester on Lipopolysaccharide-Induced Intestinal Barrier Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Tao,Xi-Wang Liu,Zhen-Dong Zhang,Ning Ma,Xiao-Rong Lu,Wen-Bo Ge,Jian-Yong Li,Ya-Jun YangPubMed 38139262
- Iranian journal of public health 52:1962-19722023Licochalcone A Inhibits Proliferation and Metastasis of Colon Cancer by Regulating miR-1270/ADAM9/Akt/NF-κB axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanghai Pan,Hongjin Chen,Bolin YangPubMed 38033851
- BMC complementary medicine and therapies 23:4302023Prevention of STAT3-related pathway in SK-N-SH cells by natural product astaxanthin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShao-Qian Sun,Feng-Xiang Du,Li-Hua Zhang, Hao-Shi,Fu-Ying Gu,Yu-Lin Deng,Yi-Zhi JiPubMed 38031104
- Inflammopharmacology 32:715-7312023L-Carnitine augments probenecid anti-inflammatory effect in monoiodoacetate-induced knee osteoarthritis in rats: involvement of miRNA-373/P2X7/NLRP3/NF-κB milieu.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRawan Mahfouz,Safaa H El-Rewini,Asser I Ghoneim,Eman Sheta,Mennatallah A Ali,Sherihan Salaheldin Abdelhamid IbrahimPubMed 37994991
- Journal of orthopaedic surgery and research 18:8762023BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Ning,Huihui Guo,Mingming Ma,Zhengang ZhaPubMed 37980502
- Cellular & molecular biology letters 28:912023Irf7 regulates the expression of Srg3 and ferroptosis axis aggravated sepsis-induced acute lung injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinyu Ling,Shiyou Wei,Dandan Ling,Siqi Cao,Rui Chang,Qiuyun Wang,Zhize YuanPubMed 37946128
- BMC pulmonary medicine 23:4362023FUT3 facilitates glucose metabolism of lung adenocarcinoma via activation of NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanlan Lin,Xiaohui Chen,Guofu Lin,Luyang Chen,Yuan Xu,Yiming ZengPubMed 37946130
- Histology and histopathology 39:623-6322023mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianguo Song,Juan Dai,Xueping Chen,Fei Ding,Yanbo Ding,Liang Ma,Liwen ZhangPubMed 37916940
- Aging 15:12120-121352023USP14 predicts poorer survival outcomes and promotes tumor progression in endometrial carcinoma by activating NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojin Gong,Li Jia,Lili Zhou,Tongxiu HuPubMed 37917013
- Journal of translational medicine 21:7702023Lycium barbarum glycopeptide alleviates neuroinflammation in spinal cord injury via modulating docosahexaenoic acid to inhibiting MAPKs/NF-kB and pyroptosis pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanfeng Jiang,Zhong Zeng,He He,Mei Li,Yuanxiang Lan,Jianwen Hui,Pengfei Bie,Yanjun Chen,Hao Liu,Heng Fan,Hechun XiaPubMed 37907930
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Expected | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Rat | Expected | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/30 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Recommended products
Anti-NF-kB p65 (phospho S536) antibody EP2294Y is a rabbit monoclonal antibody that is used in NF-kB p65 (phospho S536) Western blot, IHC, ICC/IF, Flow cytometry, IP. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Cited in over 240 publications
- Trusted since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP2294Y
- Purification technique
- Affinity purification Protein A
- Specificity
Stimulation may be required to allow detection of the phosphorylated protein.
We recommend using NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes as a positive control.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot blot, IP, WB in human, mouse, rat samples.
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) specifically detects NF-kB p65 Phospho-S536 (UniProt ID: Q04206; Molecular weight: 60kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) has been cited over 247 times in peer reviewed journals and is trusted by the scientific community.
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) has 7 independent reviews from customers.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--immunoprecipitation-img137564.jpg "Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)")
Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)
ab76302 at 1/30 immunoprecipitating NF-kB p65 (phospho S536) in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.
Lane 1 (input): Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha (10μg)
Lane 2 (+): ab76302 + Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76302 in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.
For western blotting, ab76302 at 1/500 dilution (0.95 μg/ml) and VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution were used.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--western-blot-img11278.jpg "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)")
Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)
All lanes: Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2: C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg
Lane 3: C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--western-blot-img11548.jpg "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)")
Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)
All lanes: Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A and TNF-alpha whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-ab76302--dot-blot-img4543.jpg "Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)")
Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (ab76302)
Dot blot analysis of INF- kB p65 (phospho S536) phospho peptide (Lane 1) and NF- kB p65 non-phospho peptide (Lane 2) labeling NF-kB p65 (phospho S536) with ab76302 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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