Rabbit Recombinant Monoclonal NF-kB p65 phospho S536 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 21 publications.
Images
![Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--immunoprecipitation-img137564.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882)")
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--western-blot-img11278.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882)")
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--western-blot-img8188.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882)")
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--western-blot-img11548.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882)")
![Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--dot-blot-img4543.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (AB239882)")
Publications
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e231272023PCSK9 causes inflammation and cGAS/STING pathway activation in diabetic nephropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhicai Feng,Xiangyu Liao,Juan Peng,Jingjing Quan,Hao Zhang,Zhijun Huang,Bin YiPubMed 37561547
- NPJ biofilms and microbiomes 9:332023Limosilactobacillus mucosae-derived extracellular vesicles modulates macrophage phenotype and orchestrates gut homeostasis in a diarrheal piglet model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Li,Shuaifei Feng,Zhenyu Wang,Jinhui He,Zeyue Zhang,Huicong Zou,Zhifeng Wu,Xiangdong Liu,Hong Wei,Shiyu TaoPubMed 37280255
- Phytotherapy research : PTR 37:2405-24182023Baicalin ameliorates high fat diet-induced nonalcoholic fatty liver disease in mice via adenosine monophosphate-activated protein kinase-mediated regulation of SREBP1/Nrf2/NF-κB signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanxia Gao,Jingbo Liu,Zhili Hao,Na Sun,Jianhua Guo,Xiaozhong Zheng,Panpan Sun,Wei Yin,Kuohai Fan,Hongquan LiPubMed 36752274
- Computational and mathematical methods in medicine 2022:69603042022Tanshinone IIA May Inhibit Gastric Cancer via Affecting the Intestinal Microbiome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFenggang Lu,Yi Zhang,Pinghui Song,Yu Xu,Zhongkun Wu,Qinyu Wang,Naiying ShenPubMed 36199775
- Molecular medicine reports 26:2022Role of TRIM22 in ulcerative colitis and its underlying mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Ye,Zhongkai LuPubMed 35674157
- Cell death & disease 13:5002022Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Brandolini,Michele d'Angelo,Rubina Novelli,Vanessa Castelli,Cristina Giorgio,Anna Sirico,Pasquale Cocchiaro,Francesco D'Egidio,Elisabetta Benedetti,Claudia Cristiano,Antonella Bugatti,Anna Ruocco,Pier Giorgio Amendola,Carmine Talarico,Candida Manelfi,Daniela Iaconis,Andrea Beccari,Andreza U Quadros,Thiago M Cunha,Arnaldo Caruso,Roberto Russo,Annamaria Cimini,Andrea Aramini,Marcello AllegrettiPubMed 35614037
- Toxicology research 11:437-4502022Dimethyl fumarate ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NLRP3 inflammasome-mediated pyroptosis through enhancing Nrf2 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuayu Li,Mengyan Li,Chao Dong,Bing LiuPubMed 35782648
- Molecular medicine reports 25:2022KLF4 downregulates FGF21 to activate inflammatory injury and oxidative stress of LPS‑induced ATDC5 cells via SIRT1/NF‑κB/p53 signaling.
- Bioengineered 13:2816-28262022Calcium dobesilate alleviates renal dysfunction and inflammation by targeting nuclear factor kappa B (NF-κB) signaling in sepsis-associated acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijuan Xie,Lanji Wei,Jianying Chen,Zhong ChenPubMed 35038964
- Frontiers in pharmacology 12:7833172021Cajanol Sensitizes A2780/Taxol Cells to Paclitaxel by Inhibiting the PI3K/Akt/NF-κB Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Sui,Hairong Yang,Mingqi Guo,Wenle Li,Zheng Gong,Jing Jiang,Peiling LiPubMed 34955854
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | Dot | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Mouse | Expected | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Rat | Expected | Not recommended | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed:33440148).
Alternative names
NFKB3, RELA, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Recommended products
Rabbit Recombinant Monoclonal NF-kB p65 phospho S536 antibody. Carrier free. Suitable for IP, Dot, WB and reacts with Human, Mouse, Rat, Synthetic peptide samples. Cited in 21 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP2294Y
- Purification technique
- Affinity purification Protein A
- Specificity
Stimulation may be required to allow detection of the phosphorylated protein.
We recommend using NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes as a positive control.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239882 is the carrier-free version of Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NF-kB p65 also known as RelA is a significant component of the NF-kB protein complex. This complex usually involves a molecular weight for p65 of approximately 65 kDa. NF-kB p65 is mechanistically a transcription factor that regulates genes involved in inflammation cell survival and immune response. Expression of p65 is widely seen in various cell types including immune cells and epithelial cells suggesting its role in numerous physiological processes.
- Biological function summary
NF-kB p65 acts as part of the larger NF-kB complex usually forming a heterodimer with other family members like p50. This complex translocates to the nucleus upon activation where it binds specific DNA sequences to regulate gene expression. The p65 subunit is essential for transactivating target genes involved in immune and inflammatory responses. Its regulation is important for proper cellular functioning especially in the maintenance of immune homeostasis and inflammatory response.
- Pathways
NF-kB p65 participates in critical pathways like the NF-kB signaling and Toll-like receptor pathways. These pathways are fundamental for initiating immune responses and contribute to the regulation of apoptosis and cellular stress responses. In the context of these pathways the IKK complex plays a pivotal role in NF-kB activation leading to the phosphorylation and subsequent degradation of inhibitors that retain NF-kB p65 in the cytoplasm therefore allowing its movement to the nucleus.
- Associated diseases and disorders
NF-kB p65 is implicated in conditions such as cancer and autoimmune diseases. Its dysregulation can lead to persistent activation of inflammatory pathways contributing to tumorigenesis and chronic inflammation. In the context of these diseases proteins such as the p50 subunit and the IKK complex are often involved reflecting the importance of tight regulation of NF-kB signaling in preventing pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--immunoprecipitation-img137564.jpg "Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)")
Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302 at 1/30 immunoprecipitating NF-kB p65 (phospho S536) in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.
Lane 1 (input): Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha (10μg)
Lane 2 (+): Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302 + Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302 in Daudi whole cell lysate treated with calyculin A and tumor necrosis factor-alpha.
For western blotting, Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302 at 1/500 dilution (0.95 μg/ml) and VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution were used.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302).
All lanes: Immunoprecipitation - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302)
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--western-blot-img11278.jpg "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)")
Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
All lanes: Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302) at 1/1000 dilution
Lane 1: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg
Lane 2: C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg
Lane 3: C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--western-blot-img8188.jpg "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)")
Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
All lanes: Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882) at 1/1000 dilution
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg
Lane 2: NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--western-blot-img11548.jpg "Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)")
Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
All lanes: Western blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] (Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) treated with Calyculin A and TNF-alpha whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 65 kDa
![Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882), expandable thumbnail](https://content.abcam.com/products/images/nf-kb-p65-phospho-s536-antibody-ep2294y-bsa-and-azide-free-ab239882--dot-blot-img4543.jpg "Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)")
Dot Blot - Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] - BSA and Azide free (ab239882)
Dot blot analysis of INF- kB p65 (phospho S536) phospho peptide (Lane 1) and NF- kB p65 non-phospho peptide (Lane 2) labeling NF-kB p65 (phospho S536) with Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302 at a dilution of 1/1000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NF-kB p65 (phospho S536) antibody [EP2294Y] ab76302).
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