AB217016

Anti-NF2 / Merlin antibody

Name: Anti-NF2 / Merlin antibody (ab217016) | Abcam
Brand: Abcam Limited
SKU: AB217016
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal NF2 / Merlin antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NF2 aa 400-500 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin antibody (AB217016)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin antibody (AB217016)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat brain tissue labeling NF2 / Merlin with ab217016 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NF2 aa 400-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35240

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.

Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.

See full target information NF2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 12: PubMed36552980

2022

Expression of the Hippo Pathway Core Components in Endometrial Cancer and Its Association with Clinicopathologic Features.

Applications

Unspecified application

Species

Unspecified reactive species

Juseok Yang,Dae Hyun Song,Cho Hee Kim,Min Hye Kim,Hyen Chul Jo,Hyoeun Kim,Ji Eun Park,Jong Chul Baek

The Journal of biological chemistry 297:100848 PubMed34058200

2021

RAB11A-mediated YAP localization to adherens and tight junctions is essential for colonic epithelial integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Sayantani Goswami,Iyshwarya Balasubramanian,Luca D'Agostino,Sheila Bandyopadhyay,Radha Patel,Shail Avasthi,Shiyan Yu,James R Goldenring,Edward M Bonder,Nan Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NF2 / Merlin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NF2 / Merlin antibody (AB217016)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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