Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free

• WB

Unknown

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (AB247805)

This data was developed using ab109244, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (<a href='/en-us/products/primary-antibodies/nf2-merlin-antibody-epr25732-ab109244'>ab109244</a>) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

PC3 cell lysate at 10 µg

Predicted band size: 70 kDa

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• WB

Lab

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (AB247805)

False colour image of Western blot : Anti-NF2 / Merlin antibody [EPR2573(2)] staining at 1/50000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109244 was shown to bind specifically to NF2 / Merlin. A band was observed at 74 kDa in wild-type HepG2 cell lysates with no signal observed at this size in NF2 knockout cell line ab277859 (knockout cell lysate ab283826). To generate this image, wild-type and NF2 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (<a href='/en-us/products/primary-antibodies/nf2-merlin-antibody-epr25732-ab109244'>ab109244</a>) at 1/50000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

NF2 knockout HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human NF2 (Merlin) knockout Hep G2 cell line (<a href='/en-us/products/cell-lines/human-nf2-merlin-knockout-hep-g2-cell-line-ab277859'>ab277859</a>)

Predicted band size: 70 kDa

Observed band size: 74 kDa

false

• WB

Lab

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (AB247805)

This data was developed using ab109244, the same antibody clone in a different buffer formulation.

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)

Lane 2 : NF2 knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109244 observed at 70 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab109244 was shown to specifically react with NF2 in wild type cells as signal was lost in NF2 knockout cells. Wild-type and NF2 knockout samples were subjected to SDS-PAGE. ab109244 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (<a href='/en-us/products/primary-antibodies/nf2-merlin-antibody-epr25732-ab109244'>ab109244</a>)

Predicted band size: 70 kDa

false

• WB

Lab

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (AB247805)

This data was developed using the same antibody clone in a different buffer formulation (ab109244).

Lanes 1 - 2 : Merged signal (red and green). Green - ab109244 observed at 60 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab109244 was shown to react with NF2 in wild-type HeLa cells in western blot with loss of signal observed in NF2 knockout cell line ab261796 (NF2 knockout cell lysate ab257179). Wild-type and NF2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab109244 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (<a href='/en-us/products/primary-antibodies/nf2-merlin-antibody-epr25732-ab109244'>ab109244</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

NF2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NF2 (Merlin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-nf2-merlin-knockout-hela-cell-line-ab261796'>ab261796</a>)

Predicted band size: 70 kDa

Observed band size: 60 kDa

false

• WB

Supplier Data

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] - BSA and Azide free (AB247805)

False colour image of Western blot : Anti-NF2 / Merlin antibody [EPR2573(2)] staining at 1/50000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109244 was shown to bind specifically to NF2 / Merlin. A band was observed at 73 kDa in wild-type A549 cell lysates with no signal observed at this size in NF2 knockout cell line. To generate this image, wild-type and NF2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution. This data was developed using the same antibody clone in a different buffer formulation (ab109244).

All lanes:

Western blot - Anti-NF2 / Merlin antibody [EPR2573(2)] (<a href='/en-us/products/primary-antibodies/nf2-merlin-antibody-epr25732-ab109244'>ab109244</a>) at 1/50000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

NF2 knockout A549 cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 70 kDa

Observed band size: 73 kDa

false