Anti-NF2 / Merlin antibody [RP23040199]

• WB

Supplier Data

Western blot - Anti-NF2 / Merlin antibody [RP23040199] (AB308025)

Knockdown of NF2 was achieved by transfecting PC3 cells with NF2 specific siRNA. Western blot analysis was performed using whole cell lysates from the NF2 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ab308025, 1-2 µg/mL and Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). Reduction of signal upon siRNA mediated knock down confirms that antibody is specific to NF2.

All lanes:

Western blot - Anti-NF2 / Merlin antibody [RP23040199] (ab308025) at 1 µg/mL

Lane 1:

Untransfected PC3 cells

Lane 2:

PC3 cells transfected with non-specific scrambled siRNA

Lane 3:

PC3 NF2 knockdown cells

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2500 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

• WB

Supplier Data

Western blot - Anti-NF2 / Merlin antibody [RP23040199] (AB308025)

Western blot analysis was performed on whole cell extracts (30 µg lysate) of DU 145 (Lane 1), SH-SY5Y (Lane 2), U-2 OS (Lane 3), A-431 (Lane 4), PC-3 (Lane 5) and tissue extracts of Mouse Brain (Lane 6) and Rat Brain (Lane 7). The blots were probed with ab308025, 1 µg/mL. A 70 kDa band corresponding to NF2 (Merlin) was observed across the cell lines and tissues tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). Known quantity of protein samples were electrophoresed using 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-NF2 / Merlin antibody [RP23040199] (ab308025) at 1 µg/mL

Lane 1:

DU145 whole cell extract at 30 µg

Lane 2:

SH-SY5Y whole cell extract at 30 µg

Lane 3:

U-2 OS whole cell extract at 30 µg

Lane 4:

A-431 whole cell extract at 30 µg

Lane 5:

PC-3 whole cell extract at 30 µg

Lane 6:

Mouse brain tissue extract at 30 µg

Lane 7:

Rat brain tissue extract at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

true