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AB47378

Anti-NF2 / Merlin (phospho S518) antibody

2

(3 Reviews)

|

(1 Publication)

Rabbit Polyclonal NF2 / Merlin phospho S518 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NF2 phospho S518 aa 450-550.

View Alternative Names

SCH, NF2, Merlin, Moesin-ezrin-radixin-like protein, Neurofibromin-2, Schwannomerlin, Schwannomin

1 Images
Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB47378)
  • WB

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Western blot - Anti-NF2 / Merlin (phospho S518) antibody (AB47378)

All lanes:

Western blot - Anti-NF2 / Merlin (phospho S518) antibody (ab47378) at 1/500 dilution

Lane 1:

HuvEc cell extracts treated with IFN-a(1000U/ml, 18 hours)

Lane 2:

HuvEc cell extracts

Predicted band size: 70 kDa

Observed band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human NF2 phospho S518 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P35240

Specificity

Detects endogenous levels of Merlin only when phosphorylated at serine 518.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.
Biological function summary

The NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.

Pathways

NF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.

Mutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with WWC1 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. May act as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity. Suppresses cell proliferation and tumorigenesis by inhibiting the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex.
See full target information NF2 phospho S518
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 67:520-7 PubMed17234759

2007

Merlin/neurofibromatosis type 2 suppresses growth by inhibiting the activation of Ras and Rac.

Applications

WB

Species

Human

Helen Morrison,Tobias Sperka,Jan Manent,Marco Giovannini,Helmut Ponta,Peter Herrlich
View all publications
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