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AB203206

Anti-NFAM1 antibody

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(1 Publication)

Rabbit Polyclonal NFAM1 antibody. Suitable for IHC-P, Flow Cyt and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NFAM1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CNAIP, NFAM1, NFAT activation molecule 1, Calcineurin/NFAT-activating ITAM-containing protein, NFAT-activating protein with ITAM motif 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAM1 antibody (AB203206)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAM1 antibody (AB203206)

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human lung carcinoma tissue labeling NFAM1 using ab203206 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry - Anti-NFAM1 antibody (AB203206)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NFAM1 antibody (AB203206)

Flow cytometry analysis of Mouse splenocytes labeling NFAM1 using ab203206 at 1/100 dilution for 40 minutes (green) followed by a FITC conjugated secondary compared to control cells (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human NFAM1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8NET5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 with fluorescent detection methods.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAM1 also known as NFAT activating protein with an undefined function 1 is a protein expressed on the surface of immune cells such as B cells and T cells. It consists of approximately 46 kDa. This membrane-associated protein plays a significant role in the activation of nuclear factor of activated T-cells (NFAT) a family of transcription factors integral to immune responses. Researchers originally identified NFAM1 in immune system cells pointing to its immunological relevance.
Biological function summary

The role of NFAM1 involves important signal transduction processes that support immune cell activation and function. NFAM1 likely participates in complex interactions on the cell surface that facilitate NFAT activation although the specific molecular interactions remain under investigation. Evidence suggests that it may integrate signals from various receptors orchestrating immune cell responses and contributing to the adaptive immune system.

Pathways

NFAM1 is notably involved in the NFAT signaling pathway which regulates gene expression essential for immune cell function. This protein interacts closely with other molecules in the pathway including calcineurin a phosphatase critical for NFAT dephosphorylation. Such interactions reinforce NFAM1's role in maintaining effective immune responses. Additionally NFAM1 has a role in the broader context of the T-cell receptor signaling pathway further highlighting its importance in immune regulation.

NFAM1's involvement in autoimmune conditions and certain immunodeficiencies stands out. Researchers have linked dysregulation of NFAT signaling where NFAM1 is active to autoimmune diseases such as rheumatoid arthritis. Furthermore improper NFAM1 function may affect T cell-mediated immune responses contributing to specific immunodeficiency disorders. Investigating NFAM1's interactions with proteins such as NFATc1 and its impact on disease progression offers potential avenues for understanding and treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function in immune system as a receptor which activates via the calcineurin/NFAT-signaling pathway the downstream cytokine gene promoters. Activates the transcription of IL-13 and TNF promoters. May be involved in the regulation of B-cell, but not T-cell, development. Overexpression activates downstream effectors without ligand binding or antibody cross-linking.
See full target information NFAM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:15319 PubMed40312444

2025

The role of gut microbiota on skeletal muscle strength in LDLR knockdown aging mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Ziyang Fang,Yinjun Luo,Huiling Liu,Dianchen Zhao,Tao Feng,Xin Zhang,Songyi Mo,Xiaoyan Fang,Lingling Huang,Jinhua Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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