AB244310

Anti-NFAT1 antibody

Name: Anti-NFAT1 antibody (ab244310) | Abcam
Brand: Abcam Limited
SKU: AB244310
Price: 630 USD
Availability: InStock

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Rabbit Polyclonal NFAT1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFATC2 aa 1-200.

View Alternative Names

NFAT1, NFATP, NFATC2, NF-ATc2, NFATc2, NFAT pre-existing subunit, T-cell transcription factor NFAT1, NF-ATp

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody (AB244310)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody (AB244310)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for NFAT1 (green) using ab244310 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Formalin-fixed, paraffin-embedded human lymph node tissue stained for NFAT1 using ab244310 at 1/50 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for NFAT1 using ab244310 at 1/50 dilution in immunohistochemical analysis.

No positivity in myocytes, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for NFAT1 using ab244310 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-NFAT1 antibody (AB244310)

All lanes:

Western blot - Anti-NFAT1 antibody (ab244310) at 0.4 µg/mL

All lanes:

Human cell line CACO-2

Predicted band size: 100 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NFATC2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q13469

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAT1 also known as NFATc2 is a transcription factor involved in gene regulation with a molecular weight of approximately 100 kDa. It belongs to the NFAT (nuclear factor of activated T-cells) family and is expressed in a variety of tissues including the immune system and the nervous system. NFAT1 plays an essential role in activating specific gene expressions by translocating into the nucleus upon cell stimulation. Researchers frequently use NFAT molecular weight to confirm its presence in protein analyses such as NFAT western blot techniques.

Biological function summary

NFAT1 influences immune response and development. It acts as part of a large complex with other transcription factors to bind DNA and regulate transcription of cytokines and other critical immune molecules. In activated T-cells NFAT1 controls the expression of genes important for immune function including interleukins. In neuronal tissues it contributes to neural growth and survival highlighting its multifunctional nature in different cell types.

Pathways

NFAT1 integrates into the calcineurin signaling pathway and plays a significant role in T-cell activation. It acts downstream of the calcium signaling cascade which leads to dephosphorylation and activation by the phosphatase calcineurin. NFAT1 works closely with proteins like AP-1 and GATA to facilitate the transcription of immune-related genes. These interactions highlight NFAT1's importance in immune signaling pathways which modulate immune surveillance and homeostasis.

NFAT1 associates with autoimmune diseases and certain cancers. In autoimmune conditions such as rheumatoid arthritis aberrant NFAT1 function leads to excessive cytokine production exacerbating inflammation. The protein also links to cancer development particularly in lymphoid malignancies where dysregulated NFAT1 activity contributes to uncontrolled cell proliferation. It interacts with oncogenic kinases and other dysregulated proteins in these disorders influencing both their progression and possible therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed : 15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed : 21871017). Is involved in the negative regulation of chondrogenesis (PubMed : 35789258). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.

See full target information NFATC2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFAT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NFAT1 antibody (AB244310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT1 antibody (AB244310)

Western blot - Anti-NFAT1 antibody (AB244310)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise