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AB200819

Anti-NFAT1 (phospho S54) antibody

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(7 Publications)

Rabbit Polyclonal NFAT1 phospho S54 antibody. Suitable for WB and reacts with Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Nfatc2 pS54.

View Alternative Names

Nfat1, Nfatp, Nfatc2, NF-ATc2, NFATc2, NFAT pre-existing subunit, T-cell transcription factor NFAT1, NF-ATp

1 Images
Western blot - Anti-NFAT1 (phospho S54) antibody (AB200819)
  • WB

Supplier Data

Western blot - Anti-NFAT1 (phospho S54) antibody (AB200819)

Resolved by SDS-PAGE on a 10% Tris-glycine gel

All lanes:

Western blot - Anti-NFAT1 (phospho S54) antibody (ab200819) at 1/1000 dilution

Lane 1:

Extracts of Murine T cells were left untreated with no peptide

Lane 2:

Extracts of Murine T cells were treated with PMA and Ca2+ ionophoreionomycin with no peptide

Lane 3:

Extracts of Murine T cells were treated with PMA and Ca2+ ionophoreionomycin with the non-phosphopeptide corresponding to the phosphopeptide immunogen

Lane 4:

Extracts of Murine T cells were treated with PMA and Ca2+ ionophoreionomycin with a generic phosphoserine-containing peptide

Lane 5:

Extracts of Murine T cells were treated with PMA and Ca2+ ionophoreionomycin with the phosphopeptide immunogen

Secondary

All lanes:

Goat F(ab’)2 anti-rabbit IgG alkaline phosphatase

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Mouse Nfatc2 pS54. The exact immunogen used to generate this antibody is proprietary information.

Q60591

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab200819 was negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated NFAT1. The final product is generated by affinity chromatography using a NFAT1-derived peptide phosphorylated at serine 54.
Storage buffer
pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAT1 also known as NFATc2 is a transcription factor involved in gene regulation with a molecular weight of approximately 100 kDa. It belongs to the NFAT (nuclear factor of activated T-cells) family and is expressed in a variety of tissues including the immune system and the nervous system. NFAT1 plays an essential role in activating specific gene expressions by translocating into the nucleus upon cell stimulation. Researchers frequently use NFAT molecular weight to confirm its presence in protein analyses such as NFAT western blot techniques.
Biological function summary

NFAT1 influences immune response and development. It acts as part of a large complex with other transcription factors to bind DNA and regulate transcription of cytokines and other critical immune molecules. In activated T-cells NFAT1 controls the expression of genes important for immune function including interleukins. In neuronal tissues it contributes to neural growth and survival highlighting its multifunctional nature in different cell types.

Pathways

NFAT1 integrates into the calcineurin signaling pathway and plays a significant role in T-cell activation. It acts downstream of the calcium signaling cascade which leads to dephosphorylation and activation by the phosphatase calcineurin. NFAT1 works closely with proteins like AP-1 and GATA to facilitate the transcription of immune-related genes. These interactions highlight NFAT1's importance in immune signaling pathways which modulate immune surveillance and homeostasis.

NFAT1 associates with autoimmune diseases and certain cancers. In autoimmune conditions such as rheumatoid arthritis aberrant NFAT1 function leads to excessive cytokine production exacerbating inflammation. The protein also links to cancer development particularly in lymphoid malignancies where dysregulated NFAT1 activity contributes to uncontrolled cell proliferation. It interacts with oncogenic kinases and other dysregulated proteins in these disorders influencing both their progression and possible therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (By similarity). Is involved in the negative regulation of chondrogenesis (PubMed : 10620601). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.
See full target information Nfatc2 pS54
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:336 PubMed40597262

2025

VEGF-B-mediated myofiber types involved in high-fat diet-induced hyperglycemia through PKA-NFATs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Liu Shi,Yu-Ting Sun,Jia-Nan Sun,Jing Yue,Wei Chen,Kun Meng,Long Chen,Chang-Qing Hu,Rui Chen,Dong-Sheng Sun,Cheng-Bao Xu,Wei Yuan,Xin-Li Li,Dan Zhao,Yan Wu,Shi-Bing Xi,Xiao-Ying Zhao,Jun-Ming Tang

Nature communications 15:10095 PubMed39572569

2024

Calcium nanoparticles target and activate T cells to enhance anti-tumor function.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Zhizi Feng,Xinning Lai,Jianwen Li,Zhengwei Cao,Fangchao Jiang,Fanghui Chen,Shuyue Zhan,Feng Kong,Li Yang,Yong Teng,Wendy T Watford,Gang Zhou,Jin Xie

JCI insight 7: PubMed35603788

2022

A reengineered common chain cytokine augments CD8+ T cell-dependent immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Anirban Banerjee,Dongge Li,Yizhan Guo,Zhongcheng Mei,Christine Lau,Kelly Chen,John Westwick,Jeffery B Klauda,Adam Schrum,Eric R Lazear,Alexander S Krupnick

Cell reports 33:108500 PubMed33326785

2020

Lactate Limits T Cell Proliferation via the NAD(H) Redox State.

Applications

Unspecified application

Species

Unspecified reactive species

William J Quinn,Jing Jiao,Tara TeSlaa,Jason Stadanlick,Zhonglin Wang,Liqing Wang,Tatiana Akimova,Alessia Angelin,Patrick M Schäfer,Michelle D Cully,Caroline Perry,Piotr K Kopinski,Lili Guo,Ian A Blair,Louis R Ghanem,Michael S Leibowitz,Wayne W Hancock,Edmund K Moon,Matthew H Levine,Evgeniy B Eruslanov,Douglas C Wallace,Joseph A Baur,Ulf H Beier

EBioMedicine 41:384-394 PubMed30803933

2019

FUN14 domain-containing 1 promotes breast cancer proliferation and migration by activating calcium-NFATC1-BMI1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lianpin Wu,Donghong Zhang,Li Zhou,Yuqing Pei,Yixuan Zhuang,Wei Cui,Jiongyu Chen

Journal of cachexia, sarcopenia and muscle 8:954-973 PubMed29130633

2017

The mitochondrial metabolic reprogramming agent trimetazidine as an 'exercise mimetic' in cachectic C26-bearing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Molinari,Fabrizio Pin,Stefania Gorini,Sergio Chiandotto,Laura Pontecorvo,Fabio Penna,Emanuele Rizzuto,Simona Pisu,Antonio Musarò,Paola Costelli,Giuseppe Rosano,Elisabetta Ferraro

Molecular cancer 16:147 PubMed28851360

2017

Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca-permeable Cationic Channel in lung carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zhang,Lan Zhang,Bihua Lin,Xingxing Chai,Ronggang Li,Yuehua Liao,Xinghui Deng,Qiongru Liu,Wenli Yang,Yubo Cai,Wei Zhou,Zhichao Lin,Wenhai Huang,Meigong Zhong,Fangyong Lei,Jinhua Wu,Shuaishuai Yu,Xiaoping Li,Shangren Li,Yueyue Li,Jincheng Zeng,Wansheng Long,Dong Ren,Yanming Huang
View all publications
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Product promise

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