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AB250212

Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NFAT2 phospho S233 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NFAT2, NFATC, NFATC1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc

1 Images
Western blot - Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] - BSA and Azide free (AB250212)
  • WB

Supplier Data

Western blot - Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] - BSA and Azide free (AB250212)

This data was developed using ab180510, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NFAT2 (phospho S233) antibody [EPR2376(N)] (<a href='/en-us/products/primary-antibodies/nfat2-phospho-s233-antibody-epr2376n-ab180510'>ab180510</a>) at 1/20000 dilution

Lane 1:

Jurkat cell lysate + Calyculin A at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 10 kDa,101 kDa,14 kDa

false

  • Unconjugated

    Anti-NFAT2 (phospho S233) antibody [EPR2376(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2376(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognizes 7 isoforms.

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Reactivity data

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Product details

ab250212 is the carrier-free version of ab180510.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAT2 also known as NFATc1 is a transcription factor with a mass of approximately 100 kDa. It belongs to the Nuclear Factor of Activated T-cells (NFAT) family which plays significant roles in gene expression regulation. NFAT2 is expressed in T-cells B-cells and cardiac muscle tissues. It is activated through dephosphorylation by the enzyme calcineurin allowing it to translocate into the nucleus. NFAT2 binds to specific DNA motifs to regulate the transcription of target genes impacting various cellular processes.
Biological function summary

NFAT2 plays a significant role in the regulation of immune responses and cardiac hypertrophy. It functions as part of a complex with AP-1 (Activator Protein-1) during T-cell activation integrating signals necessary for cytokine production and immune cell differentiation. In cardiac cells NFAT2 influences the expression of genes involved in hypertrophic growth. NFAT2 contributes to the development and function of the immune system by modulating critical immune pathways and cellular proliferation.

Pathways

NFAT2 serves a vital function in the calcineurin-NFAT signaling pathway. This pathway is important for T-cell activation and is triggered by increased intracellular calcium levels leading to dephosphorylation of NFAT2. The dephosphorylated NFAT2 cooperates with proteins such as AP-1 to induce expression of cytokines like interleukin-2. NFAT2 is also linked with the MAPK signaling pathway which affects cellular proliferation and differentiation. Together these pathways highlight the integral role of NFAT2 in immune system function and cardiac development.

NFAT2 has strong connections to autoimmune diseases and cardiac hypertrophy. Aberrations in NFAT2 activity and regulation are associated with autoimmune conditions due to its central role in immune response modulation. In cardiac hypertrophy heightened NFAT2 activity may lead to pathological enlargement of heart tissue. The protein has interactions with calcineurin which also influences these conditions. Understanding these relationships helps in exploring therapeutic targets for managing immune-related diseases and cardiac dysfunctions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed : 10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).
See full target information NFATC1 phospho S233
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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