Knockout Tested Rabbit Recombinant Monoclonal NFAT4/NF-ATc3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (AB316824), expandable thumbnail](https://content.abcam.com/products/images/nfat4-nf-atc3-antibody-epr25800-39-bsa-and-azide-free-capture-ab316824--sandwich-elisa-img427880.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (AB316824)")
![Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (AB316824), expandable thumbnail](https://content.abcam.com/products/images/nfat4-nf-atc3-antibody-epr25800-39-bsa-and-azide-free-capture-ab316824--sandwich-elisa-img427879.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (AB316824)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Acts as a regulator of transcriptional activation. Binds to the TNFSF11/RANKL promoter region and promotes TNFSF11 transcription (By similarity). Binding to the TNFSF11 promoter region is increased by high levels of Ca(2+) which induce NFATC3 expression and may lead to regulation of TNFSF11 expression in osteoblasts (By similarity). Plays a role in promoting mesenteric arterial wall remodeling in response to the intermittent hypoxia-induced increase in EDN1 and ROCK signaling (By similarity). As a result NFATC3 colocalizes with F-actin filaments, translocates to the nucleus and promotes transcription of the smooth muscle hypertrophy and differentiation marker ACTA2 (By similarity). Promotes lipopolysaccharide-induced apoptosis and hypertrophy in cardiomyocytes (By similarity). Following JAK/STAT signaling activation and as part of a complex with NFATC4 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity). In conjunction with NFATC4, involved in embryonic heart development via maintenance of cardiomyocyte survival, proliferation and differentiation (By similarity). Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 (PubMed:18815128). Required for thymocyte maturation during DN3 to DN4 transition and during positive selection (By similarity). Positively regulates macrophage-derived polymicrobial clearance, via binding to the promoter region and promoting transcription of NOS2 resulting in subsequent generation of nitric oxide (By similarity). Involved in Ca(2+)-mediated transcriptional responses upon Ca(2+) influx via ORAI1 CRAC channels.
Alternative names
NFAT4, NFATC3, NFAT4, NF-ATc3, NFATc3, NFATx, T-cell transcription factor NFAT4, NF-AT4, NF-AT4c
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal NFAT4/NF-ATc3 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR25800-39
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
ab316824 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human NFAT4/NF-ATc3. The recommended pair for sandwich ELISA is:
Capture: ab316824, Human NFAT4/NF-ATc3 Capture Antibody (unconjugated)
Detector: Anti-NFAT4/NF-ATc3 antibody [EPR25800-1] - BSA and Azide free (Detector) ab316825, Human NFAT4/NF-ATc3 Detector Antibody (unconjugated)
The reference range value is 39.06-5000 pg/mL.
The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein NFAT4 also known as NF-ATc3 or NFATc3 is a transcription factor with a molecular mass of approximately 140 kDa. It is part of the nuclear factor of activated T-cells (NFAT) family. This protein primarily localizes in the cytoplasm and nucleus and is especially abundant in immune cells like T-cells and various other cell types including neuronal tissues and cardiac cells.
- Biological function summary
NFAT4/NF-ATc3 acts as a regulator of gene expression by binding to DNA in the nucleus. It forms part of the NFAT complex working with other members of this family to regulate genes involved in cell differentiation and development. In immune cells this protein plays a role in mediating responses to external signals influencing cytokine production and aiding the regulation of immune responses. Other tissues including neurons and cardiac cells also rely on NFAT4 for specific signaling functions related to cell growth and survival.
- Pathways
NFAT4/NF-ATc3 is involved in key signaling mechanisms notably the calcium signaling pathway. This pathway works closely with other proteins such as calcineurin which dephosphorylates NFAT proteins leading to their activation and nuclear translocation. Additionally interactions within the Ras/MAPK pathway highlight NFAT4's role in cell proliferation and differentiation processes interconnecting with various signaling proteins that drive these pathways.
- Associated diseases and disorders
Abnormal NFAT4 activity links to immune-related conditions and cardiac hypertrophy. Its dysregulation associates with autoimmune diseases wherein it affects T-cell activation and inflammation. In cardiac disorders excessive activation of NFAT4 in conjunction with calcineurin contributes to maladaptive cardiac growth presenting a potential therapeutic target for hypertrophic heart conditions. The understanding of NFAT4's involvement in these diseases emphasizes its significance in developing targeted treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (ab316824), expandable thumbnail](https://content.abcam.com/products/images/nfat4-nf-atc3-antibody-epr25800-39-bsa-and-azide-free-capture-ab316824--sandwich-elisa-img427880.jpg "Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (ab316824)")
Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (ab316824)
Interpolated concentrations of native NFAT4/NF-ATc3 in human control wild type HEK293T (human embryonic kidney epithelial cell) and NFATC3 (NFAT4) knockout HEK293T cell based on 1000 µg/mL extract loads. The concentrations of NFAT4/NF-ATc3 were measured in duplicate and interpolated from the NFAT4/NF-ATc3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NFAT4/NF-ATc3 concentration was determined to be 4968.8 pg/mL in wild type HEK293T extract (Human wild-type HEK293T cell line Human wild-type HEK-293T cell line ab255449) and 430.8 pg/mL in NFATC3 (NFAT4) knockout HEK293T extract (Human NFATC3 (NFAT4) knockout HEK293T cell line Human NFATC3 (NFAT4) knockout HEK-293T cell line ab267300).
![Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (ab316824), expandable thumbnail](https://content.abcam.com/products/images/nfat4-nf-atc3-antibody-epr25800-39-bsa-and-azide-free-capture-ab316824--sandwich-elisa-img427879.jpg "Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (ab316824)")
Sandwich ELISA - Anti-NFAT4/NF-ATc3 antibody [EPR25800-39] - BSA and Azide free (Capture) (ab316824)
Sandwich ELISA of Human NFAT4/NF-ATc3 Antibody Pair - BSA and Azide free ab316823 with the capture antibody ab316824 dilution at 2 ug/mL and detector antibody Anti-NFAT4/NF-ATc3 antibody [EPR25800-1] - BSA and Azide free (Detector) ab316825 dilution at 0.5 ug/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com