AB110995

Anti-NFAT5 antibody

Name: Anti-NFAT5 antibody (ab110995) | Abcam
Brand: Abcam Limited
SKU: AB110995

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(2 Publications)

Rabbit Polyclonal NFAT5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NFAT5.

View Alternative Names

KIAA0827, TONEBP, NFAT5, Nuclear factor of activated T-cells 5, NF-AT5, T-cell transcription factor NFAT5, Tonicity-responsive enhancer-binding protein, TonE-binding protein, TonEBP

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT5 antibody (AB110995)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT5 antibody (AB110995)

ab110995, at a 1/50 dilution, staining NFAT5 in paraffin-embedded Human breast carcinoma tissue, in the absence (left) and presence (right) of the immunizing peptide.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human NFAT5. The exact immunogen used to generate this antibody is proprietary information.

O94916

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab110995 is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NFAT5 also known as TonEBP (tonicity-responsive enhancer binding protein) is a transcription factor belonging to the nuclear factor of activated T-cells (NFAT) family. It is involved in mechanistic functions through its role in the osmoregulatory mechanism aiding cells to adapt to changes in osmotic stress by regulating gene expression related to maintaining ionic and osmotic balance. NFAT5 has a molecular mass of approximately 170 kDa and is primarily expressed in kidney medulla cells where osmotic stress is prevalent.

Biological function summary

NFAT5 helps regulate the cellular response to hypertonic stress in several tissues including the kidneys brain and immune cells. It acts as a transcriptional activator that induces the expression of osmoprotective genes such as the sodium/myo-inositol transporter and aldose reductase. NFAT5 functions not as part of a larger complex but as an independent transcription factor which directly binds to enhancers within target gene promotors playing a critical role in cellular adaptation to hyperosmotic conditions.

Pathways

NFAT5 plays a significant role in the hypertonicity-induced signaling pathway and the osmoprotective pathway. Through these it influences key cellular processes by modulating the transcription of genes necessary for adaptation to osmotic stress. It stands in relation to other NFAT family members like NFATc1 and NFATc2 but distinctively differs as it does not rely directly on calcium and calcineurin for its activation unlike its relatives involved in the calcineurin/NFAT pathway.

NFAT5 has implications in cardiovascular diseases and diabetes mellitus. It's involved in cellular responses to hypertonic stress in various disease states notably influencing the vascular smooth muscle cell function in hypertension. Additionally NFAT5's regulation of osmoprotective genes connects it to metabolic disorders like diabetes where osmotic stress responses are disturbed. Interactions between NFAT5 and other proteins like TFEB (Transcription Factor EB) occur during conditions related to metabolic disorders indicating its broad relevance in stress-related pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed : 10377394). Mediates the transcriptional response to hypertonicity (PubMed : 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed : 22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA : RNA hybrid and the associated non-template single-stranded DNA (PubMed : 34049076).

See full target information NFAT5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:780727 PubMed34956208

2021

NFAT5 Controls the Integrity of Epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid Muhammad,Delicia Xavier,Stefan Klein-Hessling,Muhammad Azeem,Tabea Rauschenberger,Krisna Murti,Andris Avots,Matthias Goebeler,Matthias Klein,Tobias Bopp,Malte Sielaff,Stefan Tenzer,Sigrid Möckel,José Aramburu,Cristina López-Rodríguez,Andreas Kerstan,Edgar Serfling

Cancer research 72:1865-77 PubMed22350417

2012

miRNA signatures associate with pathogenesis and progression of osteosarcoma.

Applications

IHC-P

Species

Human

Kevin B Jones,Zaidoun Salah,Sara Del Mare,Marco Galasso,Eugenio Gaudio,Gerard J Nuovo,Francesca Lovat,Kimberly LeBlanc,Jeff Palatini,R Lor Randall,Stefano Volinia,Gary S Stein,Carlo M Croce,Jane B Lian,Rami I Aqeilan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NFAT5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFAT5 antibody (AB110995)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise