Anti-NFAT5 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-NFAT5 antibody (AB226308)

NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226308 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226308 at 0.4 μg/ml.

Lane 1 : ab226308 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-NFAT5 antibody (ab226308)

Predicted band size: 165 kDa

false

• WB

Supplier Data

Western blot - Anti-NFAT5 antibody (AB226308)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-NFAT5 antibody (ab226308) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 165 kDa

true

Exposure time: 30s