Rabbit Polyclonal NFAT5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NFAT5 aa 1450 to C-terminus.
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- Cell death & disease 10:9482019Transcription factor NFAT5 contributes to the glycolytic phenotype rewiring and pancreatic cancer progression via transcription of PGK1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongsheng Jiang,Ruizhe He,Yuhong Jiang,Dejun Liu,Lingye Tao,Minwei Yang,Chaoyi Lin,Yang Shen,Xueliang Fu,Jianyu Yang,Jiao Li,Yanmiao Huo,Rong Hua,Wei Liu,Junfeng Zhang,Baiyong Shen,Zhigang Zhang,Yongwei SunPubMed 31827081
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NFAT5 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94916
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000.00000 - 1/25000.00000 | Notes - |
Associated Products
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Target data
Function
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394). Mediates the transcriptional response to hypertonicity (PubMed:10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed:22266867). Also involved in the DNA damage response by preventing formation of R-loops; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA (PubMed:34049076).
Alternative names
KIAA0827, TONEBP, NFAT5, Nuclear factor of activated T-cells 5, NF-AT5, T-cell transcription factor NFAT5, Tonicity-responsive enhancer-binding protein, TonE-binding protein, TonEBP
Recommended products
Rabbit Polyclonal NFAT5 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NFAT5 aa 1450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NFAT5 aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94916
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab226308 was affinity purified using an epitope specific to NFAT5 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NFAT5 also known as TonEBP (tonicity-responsive enhancer binding protein) is a transcription factor belonging to the nuclear factor of activated T-cells (NFAT) family. It is involved in mechanistic functions through its role in the osmoregulatory mechanism aiding cells to adapt to changes in osmotic stress by regulating gene expression related to maintaining ionic and osmotic balance. NFAT5 has a molecular mass of approximately 170 kDa and is primarily expressed in kidney medulla cells where osmotic stress is prevalent.
- Biological function summary
NFAT5 helps regulate the cellular response to hypertonic stress in several tissues including the kidneys brain and immune cells. It acts as a transcriptional activator that induces the expression of osmoprotective genes such as the sodium/myo-inositol transporter and aldose reductase. NFAT5 functions not as part of a larger complex but as an independent transcription factor which directly binds to enhancers within target gene promotors playing a critical role in cellular adaptation to hyperosmotic conditions.
- Pathways
NFAT5 plays a significant role in the hypertonicity-induced signaling pathway and the osmoprotective pathway. Through these it influences key cellular processes by modulating the transcription of genes necessary for adaptation to osmotic stress. It stands in relation to other NFAT family members like NFATc1 and NFATc2 but distinctively differs as it does not rely directly on calcium and calcineurin for its activation unlike its relatives involved in the calcineurin/NFAT pathway.
- Associated diseases and disorders
NFAT5 has implications in cardiovascular diseases and diabetes mellitus. It's involved in cellular responses to hypertonic stress in various disease states notably influencing the vascular smooth muscle cell function in hypertension. Additionally NFAT5's regulation of osmoprotective genes connects it to metabolic disorders like diabetes where osmotic stress responses are disturbed. Interactions between NFAT5 and other proteins like TFEB (Transcription Factor EB) occur during conditions related to metabolic disorders indicating its broad relevance in stress-related pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NFAT5 antibody (ab226308)
Cell lysates were prepared using NETN lysis buffer.
All lanes: Western blot - Anti-NFAT5 antibody (ab226308) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 165 kDa
Exposure time: 30s
Immunoprecipitation - Anti-NFAT5 antibody (ab226308)
NFAT5 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226308 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226308 at 0.4 μg/ml.
Lane 1: ab226308 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-NFAT5 antibody (ab226308)
Predicted band size: 165 kDa
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