Anti-NFAT5 antibody - N-terminal

• WB

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Western blot - Anti-NFAT5 antibody - N-terminal (AB137407)

5% SDS PAGE

All lanes:

Western blot - Anti-NFAT5 antibody - N-terminal (ab137407) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 165 kDa

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• WB

CiteAb

Western blot - Anti-NFAT5 antibody - N-terminal (AB137407)

NFAT5 western blot using anti-NFAT5 antibody - N-terminal ab137407. Publication image and figure legend from Zhang, Y., Qian, X., et al., 2020, Theranostics, PubMed 31903118.

ab137407 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137407 please see the product overview.

ASIC1a induces nuclear translocation of NFATc3 by mediating Ca2+ influx. (A) Semi-quantitative analysis of NFATc1-4 and NFAT5 expressions in human normal and RA synovial tissues by integral optical density (IOD). (B) Semi-quantitative analysis of NFATc1-4 and NFAT5 expressions in rat ankle synovium by integral optical density (IOD). (C) Western blot analysis of total, nuclear, and cytoplasmic NFATc1-4 and NFAT5 expressions in NSF and RASF. (D) Western blot analysis of total, nuclear, and cytoplasmic NFATc1-4 expressions in RASF in an acidic environment (pH 6.0). (E) Western blot analysis of total NFATc3 (tNFATc3), nuclear NFATc3 (nNFATc3), and cytoplasmic NFATc3 (cNFATc3) expression in RASF-ASIC1a transfectants treated with pH 6.0 for 6 h. (F) Western blot analysis of total NFATc3 (tNFATc3), nuclear NFATc3 (nNFATc3) and cytoplasmic NFATc3 (cNFATc3) expression in ASIC1a shRNA transfectants treated with pH 6.0 for 6 h. (G) Western blot analysis of Nuclear NFATc3 (nNFATc3) proteins in RASF with 1 mM EGTA, 10 μM BAPTA-AM, 5 μM verapamil and HMA. (H) Western blot analysis of total, nuclear and cytoplasmic NFATc1-4 proteins in with 100 nM PcTx-1 were used in the experiment. (I) Immunofluorescence analysis of NFATc1-4 expressions in RASF treated with pH 6.0 + 100 nM PcTx-1. The scale bars are 20 μm. (J) Immunofluorescence analysis of NFATc3 expression in RASF with 1 mM EGTA, 10 μM BAPTA-AM, 5 μM verapamil and HMA. The scale bars are 20 μm. (K) Immunohistochemistry analysis of NFATc3 expression in rat ankle synovium on day 41 (n = 8). Student´s t-test or one-way ANOVA was used for statistical analysis, and data are expressed as mean ± SEM for three separate experiments.

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