JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327205

Anti-NFATC2 Antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal NFAT1 antibody. Suitable for IHC-P, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFATC2.

View Alternative Names

NFAT1, NFATP, NFATC2, NF-ATc2, NFATc2, NFAT pre-existing subunit, T-cell transcription factor NFAT1, NF-ATp

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC2 Antibody (AB327205)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC2 Antibody (AB327205)

Immunohistochemical staining of human cerebral cortex using ab327205 at 1 : 50–1 : 200 dilution, showing low expression as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC2 Antibody (AB327205)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC2 Antibody (AB327205)

Immunohistochemical staining of human lymph node using ab327205 at 1 : 50–1 : 200 dilution, showing high expression.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC2 Antibody (AB327205)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC2 Antibody (AB327205)

Immunohistochemistry analysis in human lymph node and cerebral cortex tissues using ab327205 at 1 : 50–1 : 200 dilution. Corresponding NFATC2 RNA-seq data are presented for the same tissues.

ChIP - Anti-NFATC2 Antibody (AB327205)
  • ChIP

Supplier Data

ChIP - Anti-NFATC2 Antibody (AB327205)

ChIP-Exo-Seq composite graph for ab327205 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human NFATC2.

Q13469

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed : 15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed : 21871017). Is involved in the negative regulation of chondrogenesis (PubMed : 35789258). Recruited by AKAP5 to ORAI1 pore-forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.
See full target information NFATC2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com