JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB327283

Anti-NFATC4 Antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal NFATC4 antibody. Suitable for ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NFATC4.

View Alternative Names

NFAT3, NFATC4, NF-ATc4, NFATc4, T-cell transcription factor NFAT3, NF-AT3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-NFATC4 Antibody (AB327283)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NFATC4 Antibody (AB327283)

Immunofluorescent staining of human cell line HUVEC TERT2 using ab327283 at 0.25–2 µg/ml, showing localization to nuclear speckles & cytosol.

ChIP - Anti-NFATC4 Antibody (AB327283)
  • ChIP

Supplier Data

ChIP - Anti-NFATC4 Antibody (AB327283)

ChIP-Exo-Seq composite graph for ab327283 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human NFATC4.

Q14934

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems (PubMed : 11514544, PubMed : 11997522, PubMed : 17213202, PubMed : 17875713, PubMed : 18668201, PubMed : 25663301, PubMed : 7749981). Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed : 18347059, PubMed : 18668201, PubMed : 7749981). Along with NFATC3, involved in embryonic heart development. Following JAK/STAT signaling activation and as part of a complex with NFATC3 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity). Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (By similarity). Transactivates many genes involved in the cardiovascular system, including AGTR2, NPPB/BNP (in synergy with GATA4), NPPA/ANP/ANF and MYH7/beta-MHC (By similarity). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (By similarity). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during the developmental critical period, when auditory neurons depend on afferent input for survival (By similarity). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP) (PubMed : 25663301). Plays a role in adipocyte differentiation (PubMed : 11997522). May be involved in myoblast differentiation into myotubes (PubMed : 17213202). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription (PubMed : 11514544). Binds to PPARG gene promoter and regulates its activity (PubMed : 11997522). Binds to PPARG and REG3G gene promoters (By similarity).
See full target information NFATC4
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com