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AB99431

Anti-NFATC4 antibody

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(12 Publications)

Rabbit Polyclonal NFATC4 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human NFATC4 aa 300-350.

View Alternative Names

NFAT3, NFATC4, NF-ATc4, NFATc4, T-cell transcription factor NFAT3, NF-AT3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC4 antibody (AB99431)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFATC4 antibody (AB99431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NFATC4 with ab99431 at 1/1000 (0.2µg/ml). Detection : DAB.

Immunoprecipitation - Anti-NFATC4 antibody (AB99431)
  • IP

Unknown

Immunoprecipitation - Anti-NFATC4 antibody (AB99431)

Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99431 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot : NFAT3 antibody (ab99431) at 0.4 µg/ml

developed using the ECL technique

Exposure time : 10 seconds

Predicted band size : 95 kDa

All lanes:

Immunoprecipitation - Anti-NFATC4 antibody (ab99431)

Predicted band size: 95 kDa

false

Western blot - Anti-NFATC4 antibody (AB99431)
  • WB

Unknown

Western blot - Anti-NFATC4 antibody (AB99431)

All lanes:

Western blot - Anti-NFATC4 antibody (ab99431) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 95 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NFATC4 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q14934

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems (PubMed : 11514544, PubMed : 11997522, PubMed : 17213202, PubMed : 17875713, PubMed : 18668201, PubMed : 25663301, PubMed : 7749981). Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed : 18347059, PubMed : 18668201, PubMed : 7749981). Along with NFATC3, involved in embryonic heart development. Following JAK/STAT signaling activation and as part of a complex with NFATC3 and STAT3, binds to the alpha-beta E4 promoter region of CRYAB and activates transcription in cardiomyocytes (By similarity). Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (By similarity). Transactivates many genes involved in the cardiovascular system, including AGTR2, NPPB/BNP (in synergy with GATA4), NPPA/ANP/ANF and MYH7/beta-MHC (By similarity). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (By similarity). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during the developmental critical period, when auditory neurons depend on afferent input for survival (By similarity). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP) (PubMed : 25663301). Plays a role in adipocyte differentiation (PubMed : 11997522). May be involved in myoblast differentiation into myotubes (PubMed : 17213202). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription (PubMed : 11514544). Binds to PPARG gene promoter and regulates its activity (PubMed : 11997522). Binds to PPARG and REG3G gene promoters (By similarity).
See full target information NFATC4
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology and pharmacology 100:104138 PubMed37137421

2023

Disruption of the mouse liver epitranscriptome by long-term aroclor 1260 exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Kellianne M Piell,Belinda J Petri,Kimberly Z Head,Banrida Wahlang,Raobo Xu,Xiang Zhang,Jianmin Pan,Shesh N Rai,Kalpani de Silva,Julia H Chariker,Eric C Rouchka,Min Tan,Yan Li,Matthew C Cave,Carolyn M Klinge

Communications biology 5:934 PubMed36085302

2022

Biomimetic cardiac tissue culture model (CTCM) to emulate cardiac physiology and pathophysiology ex vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M Miller,Moustafa H Meki,Ahmed Elnakib,Qinghui Ou,Riham R E Abouleisa,Xian-Liang Tang,Abou Bakr M Salama,Ahmad Gebreil,Cindy Lin,Hisham Abdeltawab,Fahmi Khalifa,Bradford G Hill,Najah Abi-Gerges,Roberto Bolli,Ayman S El-Baz,Guruprasad A Giridharan,Tamer M A Mohamed

Nature cardiovascular research 1:577-591 PubMed39195867

2022

Piezo1 is the cardiac mechanosensor that initiates the cardiomyocyte hypertrophic response to pressure overload in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Yan Yu,Hutao Gong,Scott Kesteven,Yang Guo,Jianxin Wu,Jinyuan Vero Li,Delfine Cheng,Zijing Zhou,Siiri E Iismaa,Xenia Kaidonis,Robert M Graham,Charles D Cox,Michael P Feneley,Boris Martinac

iScience 25:103978 PubMed35295812

2022

The role of long noncoding RNA Nron in atherosclerosis development and plaque stability.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Du,Cheng Wang,Liuye Yang,Bing Liu,Zhe Zheng,Liu Yang,Fengxiao Zhang,Jiangtong Peng,Dan Huang,Kai Huang

eLife 10: PubMed34190686

2021

The Ca-activated cation channel TRPM4 is a positive regulator of pressure overload-induced cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Guo,Ze-Yan Yu,Jianxin Wu,Hutao Gong,Scott Kesteven,Siiri E Iismaa,Andrea Y Chan,Sara Holman,Silvia Pinto,Andy Pironet,Charles D Cox,Robert M Graham,Rudi Vennekens,Michael P Feneley,Boris Martinac

Aging cell 20:e13416 PubMed34117818

2021

Q134R: Small chemical compound with NFAT inhibitory properties improves behavioral performance and synapse function in mouse models of amyloid pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Pradoldej Sompol,Jenna L Gollihue,Susan D Kraner,Irina A Artiushin,Ryan A Cloyd,Emad A Chishti,Shon A Koren,Grant K Nation,Jose F Abisambra,Orsolya Huzian,Lajos I Nagy,Miklos Santha,Laszlo Hackler,Laszlo G Puskas,Christopher M Norris

Oncogene 40:3136-3151 PubMed33824473

2021

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyi Lao,Xiaozhen Zhang,Tao Ma,Jian Xu,Hanshen Yang,Yi Duan,Honggang Ying,Xiaoyu Zhang,Chengxiang Guo,Junyu Qiu,Xueli Bai,Tingbo Liang

Journal of hepatology 73:1333-1346 PubMed32717288

2020

Targeting NFATc4 attenuates non-alcoholic steatohepatitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Du,Xiaojing Wang,Lin Yuan,Bing Liu,Xiaoxiang Mao,Dandan Huang,Liu Yang,Kun Huang,Fengxiao Zhang,Yan Wang,Xi Luo,Cheng Wang,Jiangtong Peng,Minglu Liang,Dan Huang,Kai Huang

Molecular medicine reports 21:607-614 PubMed31789412

2019

Inhibition of calcineurin/NFATc4 signaling attenuates ventilator‑induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Xiang-Zhi Fang,Xiao-Tian Liu,Yong-Feng Zheng,Yun-Bin Xie,Xiao-Dong Ma,Yan Xia,Dong-Hua Shao

Frontiers in pharmacology 9:1163 PubMed30364197

2018

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Meili Lu,Bin Leng,Xin He,Zhen Zhang,Hongxin Wang,Futian Tang
View all publications
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