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Rabbit Recombinant Monoclonal NFATC4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human samples.

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Images

Western blot - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (AB307816), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (AB307816), expandable thumbnail
  • Western blot - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (AB307816), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-PICC/IFIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Recombinant fragment - Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Recombinant fragment - Human, Rat

Dilution info

-

Notes

-

Target data

Function

Ca(2+)-regulated transcription factor that is involved in several processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems (PubMed:7749981, PubMed:11514544, PubMed:11997522, PubMed:17875713, PubMed:17213202, PubMed:18668201, PubMed:25663301). Involved in T-cell activation, stimulating the transcription of cytokine genes, including that of IL2 and IL4 (PubMed:7749981, PubMed:18668201, PubMed:18347059). Along with NFATC3, involved in embryonic heart development. Involved in mitochondrial energy metabolism required for cardiac morphogenesis and function (By similarity). Transactivates many genes involved in the cardiovascular system, including AGTR2, NPPB/BNP (in synergy with GATA4), NPPA/ANP/ANF and MYH7/beta-MHC (By similarity). Involved in the regulation of adult hippocampal neurogenesis. Involved in BDNF-driven pro-survival signaling in hippocampal adult-born neurons. Involved in the formation of long-term spatial memory and long-term potentiation (By similarity). In cochlear nucleus neurons, may play a role in deafferentation-induced apoptosis during the developmental critical period, when auditory neurons depend on afferent input for survival (By similarity). Binds to and activates the BACE1/Beta-secretase 1 promoter, hence may regulate the proteolytic processing of the amyloid precursor protein (APP) (PubMed:25663301). Plays a role in adipocyte differentiation (PubMed:11997522). May be involved in myoblast differentiation into myotubes (PubMed:17213202). Binds the consensus DNA sequence 5'-GGAAAAT-3' (Probable). In the presence of CREBBP, activates TNF transcription (PubMed:11514544). Binds to PPARG gene promoter and regulates its activity (PubMed:11997522). Binds to PPARG and REG3G gene promoters (By similarity).

Alternative names

NF-ATc4, NFATc4, T-cell transcription factor NFAT3, NF-AT3, NFAT3, NFATC4

Recommended products

Rabbit Recombinant Monoclonal NFATC4 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Recombinant fragment - Human, Human samples.

Alternative names

NF-ATc4, NFATc4, T-cell transcription factor NFAT3, NF-AT3, NFAT3, NFATC4

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24998-73

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (ab307816), expandable thumbnail

    Western blot - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (ab307816)

    This data was developed using 307815, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 35847954; PMID: 17577925; PMID: 34445390). 

    Low expression: HepG2 (Human Protein Atlas Database), HaCaT (PMID: 27893713)

    All lanes: Western blot - Anti-NFATC4 antibody [EPR24998-73] (Anti-NFATC4 antibody [EPR24998-73] ab307815) at 1/1000 dilution

    Lane 1: Untreated A-204 (human muscle rhabdomyosarcoma cell) whole cell lysate at 80 µg

    Lane 2: A-204 treated with 10uM MG-132 for 4h whole cell lysate at 80 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

    Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 80 µg

    Lane 5: HaCaT (human skin keratinocyte) whole cell lysate at 80 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 80-150 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (ab307816), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (ab307816)

    This data was developed using Anti-NFATC4 antibody [EPR24998-73] ab307815, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2(human hepatocellular carcinoma epithelial cell, Left ) / A-204(human muscle rhabdomyosarcoma cell, Right) cells labelling NFATC4 with Anti-NFATC4 antibody [EPR24998-73] ab307815 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Low expression: HepG2 (Human Protein Atlas Database)

  • Western blot - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (ab307816), expandable thumbnail

    Western blot - Anti-NFATC4 antibody [EPR24998-73] - BSA and Azide free (ab307816)

    This data was developed using 307815, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody does not cross-react with human NFATC1, NFATC2, and NFATC3.

    All lanes: Western blot - Anti-NFATC4 antibody [EPR24998-73] (Anti-NFATC4 antibody [EPR24998-73] ab307815) at 1/1000 dilution

    Lane 1: Human NFATC4 fragment at 10 ng

    Lane 2: His-tagged human NFATC3 fragment at 10 ng

    Lane 3: His-tagged human NFATC1 fragment at 10 ng

    Lane 4: His-tagged human NFATC2 fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30 kDa

    Exposure time: 7.75s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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