Anti-NFE2 Antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 Antibody (AB327173)

Immunohistochemical staining of human bone marrow using ab327173 at 1 : 50–1 : 200 dilution, showing moderate to strong nuclear positivity in hematopoietic cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 Antibody (AB327173)

Immunohistochemistry analysis in human bone marrow and liver tissues using ab327173 at 1 : 50–1 : 200 dilution. Corresponding NFE2 RNA-seq data are presented for the same tissues.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 Antibody (AB327173)

Immunohistochemical staining of human testis using ab327173 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in cells in seminiferous ducts.

• ChIP

Supplier Data

ChIP - Anti-NFE2 Antibody (AB327173)

ChIP-Exo-Seq composite graph for ab327173 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-NFE2 Antibody (AB327173)

Western blot analysis in control (vector only transfected HEK293T lysate) and NFE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) using ab327173 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-NFE2 Antibody (ab327173)

Lane 2:

vector only transfected HEK293T lysate

Lane 3:

NFE2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 41.47 kDa

false

• WB

Supplier Data

Western blot - Anti-NFE2 Antibody (AB327173)

Western blot analysis in human cell line HEL using ab327173 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-NFE2 Antibody (ab327173)

All lanes:

Human cell line HEL

Predicted band size: 41.47 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 Antibody (AB327173)

Immunohistochemical staining of human liver using ab327173 at 1 : 50–1 : 200 dilution, showing no nuclear positivity in hepatocytes as expected.