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Rabbit Recombinant Monoclonal NFE2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Western blot - Anti-NFE2 antibody [EPR20463] (AB223845), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (AB223845), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (AB223845), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] (AB223845), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/400

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Alternative names

Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, p45 NF-E2, NFE2

Recommended products

Rabbit Recombinant Monoclonal NFE2 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Alternative names

Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, p45 NF-E2, NFE2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR20463

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-NFE2 antibody [EPR20463] (ab223845), expandable thumbnail

    Western blot - Anti-NFE2 antibody [EPR20463] (ab223845)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-NFE2 antibody [EPR20463] (ab223845) at 1/1000 dilution

    Lane 1: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 10 µg

    Lane 2: HEL (human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg

    Lane 3: TF-1 (human bone marrow erythroleukemia cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa

    Observed band size: 41 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling NFE2 with ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in neutrophils (arrow) in human placenta (PMID: 8645254) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFE2 antibody [EPR20463] (ab223845)

    Immunohistochemical analysis of paraffin-embedded malignant lymphoma of bone marrow tissue labeling NFE2 with ab223845 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on a subset of cells of malignant lymphoma of bone marrow is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] (ab223845), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NFE2 antibody [EPR20463] (ab223845)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEL (human bone marrow erythroleukemia) cell line labeling NFE2 with ab223845 at 1/400 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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